An investigation of the drivers,barriers, and incentives for environmental management systems in the Malaysian food and beverage industry

Clean Technologies and Environmental Policy - Food production and consumption is one of the major causes of global environmental degradation. One way to address environmental impacts in the food...     

Bioprocess applications of model-based estimation techniques

The last decade has seen the development of a number of approaches for estimating those variables which are difficult to measure on-line in industrial process situations. Whilst a range of techniques is available, a common element is the use of process knowledge in the form of a system model. In the case of bioprocess systems, although a large range of models has been presented in the literature, their use in estimation schemes on an industrial scale has been limited. A number of reasons can be identified for their low level of utilisation. Of particular significance is the uncertainty which exists in quantifying system performance and the process-model mismatch which inevitably results. The level of ‘pre-defined model’ uncertainty, together with the knowledge gained during the course of the fermentation, serves to dictate estimator structure. The paper considers a range of estimation strategies and contrasts, through industrial applications, their performance characteristics and utility.     

Ventilation,indoor environment quality and climate—comparison of European and Singapore office buildings

Indoor environment quality audits were performed in six European countries and in Singapore using the same methodology. Five to eight office buildings were audited in each country. The audits included airflow rate measurements, energy audit, indoor air quality (VOC₁ CO₂, dust concentrations), and questionnaires to occupants. Questions to occupants addressed their health, their comfort and well-being, as well as their perception of their environment. This paper compares the results obtained from these audits, with particular attention to the effects of ventilation or air conditioning systems and climate.

The number of building related symptoms is, on the average, significantly smaller in the buildings audited in Singapore than in Europe. One of the reasons may be the non-smoking policy common in Singapore. It is also once more observed that buildings are often over-warmed in cold climates and overcooled in warm climates. Surprising, but not new, is that there are no correlations between specific air flow rates and perceived air quality or health, as long as airflow rates are within accepted standards.     

Minocycline Loaded Hybrid Composites Nanoparticles for Mesenchymal Stem Cells Differentiation into Osteogenesis

Bone transplants are used to treat fractures and increase new tissue development in bone tissue engineering. Grafting of massive implantations showing slow curing rate and results in cell death for poor vascularization. The potentials of biocomposite scaffolds to mimic extracellular matrix (ECM) and including new biomaterials could produce a better substitute for new bone tissue formation. A purpose of this study is to analyze polycaprolactone/silk fibroin/hyaluronic acid/minocycline hydrochloride (PCL/SF/HA/MH) nanoparticles initiate human mesenchymal stem cells (MSCs) proliferation and differentiation into osteogenesis. Electrospraying technique was used to develop PCL, PCL/SF, PCL/SF/HA and PCL/SF/HA/MH hybrid biocomposite nanoparticles and characterization was analyzed by field emission scanning electron microscope (FESEM), contact angle and Fourier transform infrared spectroscopy (FT-IR). The obtained results proved that the particle diameter and water contact angle obtained around 0.54 ± 0.12 to 3.2 ± 0.18 µm and 43.93 ± 10.8° to 133.1 ± 12.4° respectively. The cell proliferation and cell-nanoparticle interactions analyzed using (3-(4,5-dimethyl thiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt) MTS assay (Promega, Madison, WI, USA), FESEM for cell morphology and 5-Chloromethylfluorescein diacetate (CMFDA) dye for imaging live cells. Osteogenic differentiation was proved by expression of osteocalcin, alkaline phosphatase activity (ALP) and mineralization was confirmed by using alizarin red (ARS). The quantity of cells was considerably increased in PCL/SF/HA/MH nanoparticles when compare to all other biocomposite nanoparticles and the cell interaction was observed more on PCL/SF/HA/MH nanoparticles. The electrosprayed PCL/SF/HA/MH biocomposite nanoparticle significantly initiated increased cell proliferation, osteogenic differentiation and mineralization, which provide huge potential for bone tissue engineering.     

RB Regulates DNA Double Strand Break Repair Pathway Choice by Mediating CtIP Dependent End Resection

Inactivation of the retinoblastoma tumor suppressor gene (RB1) leads to genome instability, and can be detected in retinoblastoma and other cancers. One damaging effect is causing DNA double strand breaks (DSB), which, however, can be repaired by homologous recombination (HR), classical non-homologous end joining (C-NHEJ), and micro-homology mediated end joining (MMEJ). We aimed to study the mechanistic roles of RB in regulating multiple DSB repair pathways. Here we show that HR and C-NHEJ are decreased, but MMEJ is elevated in RB-depleted cells. After inducing DSB by camptothecin, RB co-localizes with CtIP, which regulates DSB end resection. RB depletion leads to less RPA and native BrdU foci, which implies less end resection. In RB-depleted cells, less CtIP foci, and a lack of phosphorylation on CtIP Thr847, are observed. According to the synthetic lethality principle, based on the altered DSB repair pathway choice, after inducing DSBs by camptothecin, RB depleted cells are more sensitive to co-treatment with camptothecin and MMEJ blocker poly-ADP ribose polymerase 1 (PARP1) inhibitor. We propose a model whereby RB can regulate DSB repair pathway choice by mediating the CtIP dependent DNA end resection. The use of PARP1 inhibitor could potentially improve treatment outcomes for RB-deficient cancers.     

钻进参数用于香港复杂风化花岗岩地层的界面识别

在采用仪器钻进系统对复杂风化花岗岩地层界面进行成功识别的基础上,在复杂风化花岗岩场址的勘探中进行进一步应用和检验。在该风化花岗岩地层中,强风化与中等风化花岗岩交错,地层特征相当复杂。试验采用R–20液压回转式钻机,并安装数字式钻孔过程监测系统,在钻孔过程中对穿孔参数进行自动采集。对穿孔参数的定性和定量分析表明,在此地层中,有效轴压力、钻具转速、冲洗压力、钻头位移及穿孔速率等监测参数在界面处亦具有明显的涨落,说明这些参数与界面处岩石强度的变化具有很好的响应特征,能可靠地揭示复杂地层的变化。同时,采用变斜率法对地层中的主、次界面进行识别。t检验表明,仪器钻进系统对岩土界面识别的置信度为99%。     

蓄水期库岸古滑坡的水动力学响应监测——以三峡库区泄滩滑坡为例

水库岸坡失稳是伴随水电工程建设而产生的一种地质灾害，一般认为主要是由于水库蓄水或运营导致岸坡水动力条件的不利演化造成的。国内外水库岸坡失稳的实例很多，但是岸坡的水动力条件演化过程主要以渗流计算所获得的渗流场作为依据，缺乏系统完整的现场监测资料。以三峡水库蓄水为契机，在湖北秭归县泄滩古滑坡体上建立了一套自动水文监测系统，监测内容包括水库水位、滑坡体水位、渗压与降雨量等。从监测成果来看，岸坡的水位(或渗压)上升速度明显滞后于库水位上升速度，滞后时间与岸坡的渗透性有关，滑带、滑坡影响带和基岩的渗透性相对较弱，滞后时间较长，但是滑坡体的渗透性良好，滞后时间较短；水位观测井的观测成果存在误导，需要结合观测井结构进行分析，建议尽可能使用标准管结构；降雨引起的滑坡体水位上升量和滞后时间与降雨强度关系密切，降雨强度越大水位上升量越大，水位开始上涨的时间越短。因此，对降雨的影响分析有必要考虑雨型，确定合适的基准时间单位。就泄滩滑坡而言，以小时为基本时间单位较为合适。     

Local thermal sensation and comfort study in a field environment chamber served by displacement ventilation system in the tropics

This paper presents a study of local thermal sensation (LTS) and comfort in a field environmental chamber (FEC) served by displacement ventilation (DV) system. The FEC, 11.12 m (L)×7.53 m (W)×2.60 m (H), simulates a typical office layout. A total of 60 tropically acclimatized subjects, 30 male and 30 female, were engaged in sedentary office work for 3 h. Subjects were exposed to three vertical air temperature gradients, nominally 1, 3 and 5 K/m, between 0.1 and 1.1 m heights and three room air temperatures of 20, 23 and 26 °C at 0.6 m height. The objective of this study is to investigate the mutual effect of local and overall thermal sensation (OTS) and comfort in DV environment. The results show that in a space served by DV system, at OTS close to neutral, local thermal discomfort decreased with the increase of room air temperature. The OTS of occupants was mainly affected by LTS at the arm, calf, foot, back and hand. Local thermal discomfort was affected by both LTS and OTS. At overall cold thermal sensation, all body segments prefer slightly warm sensation. At overall slightly warm thermal sensation, all body segments prefer slightly cool sensation.     

Impact of asthma,exposure period,and filters on human responses during exposures to ozone and its initiated chemistry products

The impact of asthma, exposure period, and filter condition downstream of the mixing box of air‐conditioning system on building occupants' perceptual response, work performance, and salivary α‐amylase secretion during exposures to ozone and its initiated chemistry products is studied. The experiments were conducted in a field environmental chamber (FEC) (240 m³) simulating an office environment. Experiments were conducted during periods when the air‐handling system operated with new or used pleated panel filters at constant recirculation (7/h) and ventilation (1/h) rates. Average ozone and secondary organic aerosols (ozone‐initiated chemistry products) measured during non‐asthmatic and asthmatic subjects' 3‐h exposures in the FEC were in the ranges approximately 20–37 ppb and approximately 1.6–3 μg/m³, respectively. Asthmatic subjects' perceived odor intensity and sensory (eye, nose, and throat) irritation ratings were generally lower than those of non‐asthmatic subjects, possibly explaining why asthmatic subjects accept perceived air quality more than non‐asthmatic subjects. However, asthmatic subjects' perceived physiological‐like symptom ratings (flu, chest tightness, and headache) and concentrations of secreted salivary α‐amylase were generally higher than those of non‐asthmatic subjects. Asthmatic subjects had significantly lower accuracy than non‐asthmatic subjects in a task that required higher concentration although they had higher work speed. Filter condition did not make any significant difference for subjects' responses.     

Effectiveness of a personalized ventilation system in reducing personal exposure against directly released simulated cough droplets

The inhalation intake fraction was used as an indicator to compare effects of desktop personalized ventilation and mixing ventilation on personal exposure to directly released simulated cough droplets. A cough machine was used to simulate cough release from the front, back, and side of a thermal manikin at distances between 1 and 4 m. Cough droplet concentration was measured with an aerosol spectrometer in the breathing zone of a thermal manikin. Particle image velocimetry was used to characterize the velocity field in the breathing zone. Desktop personalized ventilation substantially reduced the inhalation intake fraction compared to mixing ventilation for all investigated distances and orientations of the cough release. The results point out that the orientation between the cough source and the breathing zone of the exposed occupant is an important factor that substantially influences exposure. Exposure to cough droplets was reduced with increasing distance between cough source and exposed occupant.