Endoplasmic reticulum Ca(2+)-ATPase in microsomal vesicles isolated from bovine retinae

Active Ca2+ transport was measured in microsomal vesicles prepared from bovine retinae and was compared with that in disk membranes of the photoreceptor cells of the same retina. The 45Ca uptake was dependent on the presence of Mg(2+)-ATP and was inhibited by vanadate or when GTP substituted for ATP. The dependence of calcium uptake on the external free Ca2+ concentration gave a KM = 13 microM or a KM = 0.1 microM for disks and microsomal vesicles, respectively. A phosphorylated intermediate (E-P) of Ca(2+)-ATPase of about 100 kDa was isolated in microsomal vesicles. The E-P formation was strongly inhibited by thapsigargin and partially by 2,5-di-(-butyl)benzohydroquinone. Digestion of disks or microsomes with calpain had no effect on the phosphorylated intermediate, while digestion with trypsin produced two fragments of approximately 55 kDa and 35 kDa. These results suggest that bovine retinal microsomes contain a calcium pump belonging to the SERCA family.     

Neuroprotective Effects of Testosterone in the Hypothalamus of an Animal Model of Metabolic Syndrome

Metabolic syndrome (MetS) is known to be associated to inflammation and alteration in the hypothalamus, a brain region implicated in the control of several physiological functions, including energy homeostasis and reproduction. Previous studies demonstrated the beneficial effects of testosterone treatment (TTh) in counteracting some MetS symptoms in both animal models and clinical studies. This study investigated the effect of TTh (30 mg/kg/week for 12 weeks) on the hypothalamus in a high-fat diet (HFD)-induced animal model of MetS, utilizing quantitative RT-PCR and immunohistochemical analyses. The animal model recapitulates the human MetS features, including low testosterone/gonadotropin plasma levels. TTh significantly improved MetS-induced hypertension, visceral adipose tissue accumulation, and glucose homeostasis derangements. Within hypothalamus, TTh significantly counteracted HFD-induced inflammation, as detected in terms of expression of inflammatory markers and microglial activation. Moreover, TTh remarkably reverted the HFD-associated alterations in the expression of important regulators of energy status and reproduction, such as the melanocortin and the GnRH-controlling network. Our results suggest that TTh may exert neuroprotective effects on the HFD-related hypothalamic alterations, with positive outcomes on the circuits implicated in the control of energy metabolism and reproductive tasks, thus supporting a possible role of TTh in the clinical management of MetS.     

Engineering a pharmacologically superior form of leptin for the treatment of obesity

Leptin plays a central role in the homeostasis of body weight through its regulatory effects on appetite and energy expenditure, yet in trials as a therapeutic agent for the treatment of obesity in humans it has been disappointing. The poor clinical efficacy of leptin results from its short circulating half-life, low potency and poor solubility, necessitating large and frequent doses to obtain even modest clinical benefit. Engineered Fc-leptin immunofusins, consisting of the Fc fragment of an immunoglobulin gamma chain followed by leptin, exhibit improved pharmacological properties with very consistent and potent biological activities. Furthermore, in extending the circulating half-life of the protein in vivo from a few minutes for leptin to many hours for Fc-leptin, these proteins have the potential to reduce drastically the dosage and frequency of administration required to obtain clinical benefit. The results of this study show that the engineered leptin immunofusins described here have significantly enhanced pharmacological properties in comparison with the recombinant leptin that was used in clinical trials. As such, they could represent an important step towards a therapeutically superior form of leptin if the disappointing performance of leptin in early clinical trials was due to its poor pharmacological properties rather than any conceptual weakness in the strategy of using leptin for the treatment of obesity and its related disorders.     

Assessment of weld line performance of PP/Talc moldings produced in hot runner injection molds

Weld lines are weak regions in thermoplastic injection moldings caused by low molecular entanglement and unfavorable orientation. Their occurrence may lead to a significantly reduced mechanical performance of the products. Therefore, when weld lines are likely to occur in molded products, they must be taken into account during the mechanical and technological design processes. The weld lines become more critical when particulate fillers are compounded with the polymer. The performance of weld lines in talc‐filled polypropylene box moldings produced with a double‐gated hot runner mold is assessed in this work. The processing conditions were varied in order to cause morphology and tensile‐impact resistance changes. The weld performance at room temperature was assessed in terms of the energy absorbed in the impact tests. It was found that the performance depends on the injection temperature, the injection rate, and the orientation of the talc particles in the weld‐line plane. J. VINYL ADDIT. TECHNOL., 13:159–165, 2007. © 2007 Society of Plastics Engineers     

Temporal intensity noise characteristics and discrete numeric accuracy of analog liquid-crystal-based spatial light modulators

We investigate the temporal intensity noise characteristics of analog liquid-crystal-based spatial light modulators and how they affect the device's achievable discrete numeric accuracies in an optical computing system. First we present an analytical development that defines the concept of precision in analog computing systems, then we define a noise metric and a precision-optimal quantizer for determining the discrete numeric characteristics of the devices. Second we present an experimental discussion in which a low-noise test facility constructed for this investigation is described, and the noise characteristics of three commercially available liquid-crystal-based modulators are measured and analyzed. The accuracy implications of this measured noise are then discussed within the context of the analytical model for each modulator.     

Iso-Kinematic Maps from statistical analysis of PS-InSAR data of Piemonte, NW Italy: Comparison with geological kinematic trends

SAR interferometry based on Permanent Scatterers (PS-InSAR™) is used here to study the present crustal mobility of a large area of NW Italy, in the Piemonte region.Thirty-eight satellite scenes (ERS SAR), taken from May 1992 to January 2001, were analysed for detecting more than 2 million PS on the study area. Continuous velocity surface maps (Iso-Kinematic Maps: IKM) were obtained from geo-statistical and spatial cluster techniques (Hot Spot analysis) of PS “short-period” data, to identify relative ground motions and to compare them with “long-period” tectonic mobility trends, i.e. those inferred at regional scale over geological times (some million years).The comparison was made by individuation of homogeneous kinematic areas, represented in the IKM, and characterization of the boundaries between them (Iso-Kinematic Boundaries: IKB). The IKB were used as tools to asses if the PS-InSAR data on present-day crustal mobility could fit with the distribution of real tectonic structures or field geological elements.IKM were drawn for uplifting geological sectors of Piemonte (Maritime Alps, Gran Paradiso, Langhe) where moderate to very low seismicity is recorded, and gravitational instabilities of rock mass on mountain slopes are widespread. The land sectors have been chosen in order to test the suitability of IKM in very different geo-morphological conditions.Different types of correspondence between the IKM and the geological kinematic trend were found:

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a first type in which the kinematic trend of short-period (a decade of years, i.e. the PS-InSAR detection time span) is in agreement with a long-period tectonic trend (some million years) and seem to be driven by well known faults subparallel to the IKB. These kinematic trends can be hidden by the slope movement due to gravitational instabilities;

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a second type in which the kinematic trend of short-period does not strictly correspond to the long-period trend, but can be considered as minor-order, uplifting-subsidence cycles, even if in contrast with the long-period kinematic trend. Alternatively, the short-period kinematic trends could reflect the action of deep-seated geological forces or structures, not yet known or inferable (at least with the recorded PS-InSAR velocities) on the basis of the available geological data and models.

     

Scavenging Elemental Sb Through Addition of NbSb2 to Mm0.9Fe3.5Co0.5Sb12 Skutterudites

Our previous work identified thermal instability of p-type skutterudite as a primary cause of degradation during thermoelectric generator operation at 650°C. Residual Sb in the microstructure was believed to be the cause of diminished thermal stability, especially above the melting point of Sb (631°C). This work investigated addition of Nb to p-type skutterudite to from NbSb₂, thus scavenging elemental Sb. The results of this work are reported, along with comprehensive thermoelectric property characterization of the p-type skutterudite?+?NbSb₂ composites.     

Nanocomposites of PBAT and cellulose nanocrystals modified by in situ polymerization and melt extrusion

Cellulose nanocrystals (CNC) were successfully grafted with a low molecular weight poly(butylene glutarate) through an in situ polymerization procedure. The grafting treatment decreased the CNC hydrophilic character and increased the onset of their thermal degradation by approximately 20°C, thus increasing the possibilities of CNC application. Composites of grafted and nongrafted CNC with a poly(butylene‐adipate‐co‐terephthalate) (PBAT) matrix were prepared by melt extrusion. The CNC addition led to an increase of 50% of the tensile elastic modulus of the PBAT. In addition, dynamic mechanical thermal analysis showed that the composite with CNC retained its high modulus even at temperatures far above the glass transition temperature of PBAT. At 60°C the storage modulus of the composite with CNC was approximately 200% higher than that of the pure PBAT. Thus, in this work, nanocomposites of improved properties were obtained through a combination of in situ polymerization and melt extrusion. POLYM. ENG. SCI., 56:1339–1348, 2016. © 2016 Society of Plastics Engineers