PIn. II. Comparative evaluation of SSM/I and TMI precipitable waterestimate for the Mediterranean Sea

For pt.I see ibid., vol.39, no.12, p.2566-74 (2001). To estimate integrated precipitable water vapor along with liquid water path and water vapor effective profile (i.e. standard atmospheric profile approximation), utilizing the Special Sensor Microwave/Imager (SSM/I) and Tropical Rainfall Measuring Mission (TRMM) Microwave Imager (TMI) radiometers, an operative procedure was developed and assessed. This procedure is based on a fast nonlinear physical inversion algorithm (PIn) developed by the authors. A large data set of near-coincident TMI and SSM/I data acquisitions were collected and used to supply the procedure. Retrieved parameters were compared against retrievals achieved with well-accepted statistical algorithms, and consistency between TMI and SSM/I retrievals was confirmed. As far as TMI and SSM/I precipitable water retrieving consistency is concerned, this research revealed a linear relationship up to 20 kg/m² and a general overestimate of TMI retrieving, for higher values. A new algorithm for obtaining integrated precipitable water from TMI brightness temperatures was introduced and the goodness of its accuracy was reported. The procedure proved to be reliable and portable and its integrated precipitable water vapor retrieving was assessed to be as accurate as the best radiometric retrieving algorithms, reported in literature. For SSM/I data, developed-procedure liquid water path estimates seemed to be in good agreement with statistical retrievals. Eventually the procedure provided effective water vapor vertical profiles which belong to a deterministic distribution area characterized by an upper and lower limit; it was confirmed that SSM/I and TMI vertical profile distribution areas mainly overlap even if they are characterized by different sensitivities to profile parameters     

Modulation of Glia Activation by TRPA1 Antagonism in Preclinical Models of Migraine

Preclinical data point to the contribution of transient receptor potential ankyrin 1 (TRPA1) channels to the complex mechanisms underlying migraine pain. TRPA1 channels are expressed in primary sensory neurons, as well as in glial cells, and they can be activated/sensitized by inflammatory mediators. The aim of this study was to investigate the relationship between TRPA1 channels and glial activation in the modulation of trigeminal hyperalgesia in preclinical models of migraine based on acute and chronic nitroglycerin challenges. Rats were treated with ADM_12 (TRPA1 antagonist) and then underwent an orofacial formalin test to assess trigeminal hyperalgesia. mRNA levels of pro- and anti-inflammatory cytokines, calcitonin gene-related peptide (CGRP) and glia cell activation were evaluated in the Medulla oblongata and in the trigeminal ganglia. In the nitroglycerin-treated rats, ADM_12 showed an antihyperalgesic effect in both acute and chronic models, and it counteracted the changes in CGRP and cytokine gene expression. In the acute nitroglycerin model, ADM_12 reduced nitroglycerin-induced increase in microglial and astroglial activation in trigeminal nucleus caudalis area. In the chronic model, we detected a nitroglycerin-induced activation of satellite glial cells in the trigeminal ganglia that was inhibited by ADM_12. These findings show that TRPA1 antagonism reverts experimentally induced hyperalgesia in acute and chronic models of migraine and prevents multiple changes in inflammatory pathways by modulating glial activation.     

PIn. I. An operational nonlinear physical inversion algorithm forprecipitable and cloud liquid water estimate in nonraining conditionsover sea

For pt.II see ibid., vol.39, no.12, p.2575-86 (2001). An operational nonlinear physical inversion (PIn) algorithm for precipitable and cloud liquid water estimate is described. It is suited for a generic conical scanning satellite microwave radiometer acquisition over sea in nonraining conditions. The algorithm does not need any calibration phase and is independent of the availability of in situ data, being consistent in different geographical and climatological situations. Adopted formulation is addressed to provide observational data to help in validating water vapor and cloud fields produced by a numerical weather prediction model. Furthermore, such a technique can be utilized for the purpose of global reanalysis, improving estimates of primary fields of the hydrological cycle. A sensitivity study of the forward model and a comparison between output brightness temperatures and those from a robust numerical code are also reported. The discrepancies that result are considered acceptable with respect to instrumental constraints and computation time     

Conformationally Constrained Sialyl Analogues as New Potential Binders of h-CD22

Here, two conformationally constrained sialyl analogues were synthesized and characterized in their interaction with the inhibitory Siglec, human CD22 (h-CD22). An orthogonal approach, including biophysical assays (SPR and fluorescence), ligand-based NMR techniques, and molecular modelling, was employed to disentangle the interaction mechanisms at a molecular level. The results showed that the Sialyl-TnThr antigen analogue represents a promising scaffold for the design of novel h-CD22 inhibitors. Our findings also suggest that the introduction of a biphenyl moiety at position 9 of the sialic acid hampers canonical accommodation of the ligand in the protein binding pocket, even though the affinity with respect to the natural ligand is increased. Our results address the search for novel modifications of the Neu5Ac-α(2-6)-Gal epitope, outline new insights for the design and synthesis of high-affinity h-CD22 ligands, and offer novel prospects for therapeutic intervention to prevent autoimmune diseases and B-cell malignancies.