Tocotrienol Rich Fraction Reverses Age-Related Deficits in Spatial Learning and Memory in Aged Rats

Little is known about the effect of vitamin E on brain function. Therefore, in this study we evaluated the effect of tocotrienol rich fraction (TRF) on behavioral impairment and oxidative stress in aged rats. Thirty-six male Wistar rats (young: 3-months-old; aged: 21-months-old) were treated with either the control (olive oil) or TRF (200 mg/kg) for 3 months. Behavioral studies were performed using the open field test and Morris water maze (MWM) task. Blood was taken for assessment of DNA damage, plasma malondialdehyde (MDA) and vitamin E, and erythrocyte antioxidant enzyme activity. Brains were also collected to measure vitamin E levels. Results showed that aged rats exhibited reduced exploratory activity, enhanced anxiety and decreased spatial learning and memory compared with young rats. DNA damage and plasma MDA were increased, and vitamin E levels in plasma and brain were reduced in aged rats. Aged rats supplemented with TRF showed a markedly reduced level of anxiety, improved spatial learning and memory, reduced amount and severity of DNA damage, a reduced level of MDA, and increased levels of antioxidant enzyme activity and plasma/brain vitamin E compared with age-matched controls. In conclusion, TRF supplementation reverses spatial learning and memory decline and decreases oxidative stress in aged rats.     

Different starting times of alpha-tocopherol and gamma-tocotrienol supplementation and tumor marker enzyme activities in the rat chemically induced with cancer

1. alpha-Tocopherol (alpha-T) and gamma-tocotrienol (gamma-T) were supplemented continuously for 8 weeks in the diets of normal rats and rats chemically induced with cancer using diethylnitrosamine (DEN), 2-acetylaminofluorene (AAF) and partial hepatectomy. Hepatocarcinogenesis was followed by determining the plasma gamma-glutamyl-transpeptidase (GGT) and alkaline phosphatase (ALP) activities as well as placental glutathione S-transferase (PGST) and GGT activities histochemically, at 4-week intervals. 2. Male Rattus norvegicus were supplemented alpha-T and gamma-T at two different doses of 30 and 300 mg/kg diet. The supplementation was started at three different times: simultaneously with DEN administration; 4 weeks; and 8 weeks after DEN administration. 3. Elevation of plasma GGT activities and formation of PGST and GGT positive foci were attenuated significantly (P < 0.05) when alpha-T and gamma-T were supplemented simultaneously with cancer induction. Supplementation begun 4 and 8 weeks after cancer induction did not affect plasma enzyme activities and formation of enzyme-positive foci. 4. alpha-T was more effective than gamma-T, and a lower dose of 30 mg/kg was found to be more effective in reducing the severity of hepatocarcinogenesis.     

Optimisation of digestion method for determination of arsenic in shrimp paste sample using atomic absorption spectrometry

The microwave digestion method was developed and verified for the determination of arsenic in shrimp paste samples. Experimental design for five factors (HNO₃ and H₂O₂ volumes, sample weight, microwave power and digestion time) were used for the optimisation of sample digestion. For this purpose, two level half factorial design, which involves 16 experiments, was adopted. The concentration of arsenic was analysed by graphite furnace atomic absorption spectrometry. Design Expert® 7.0 software was used to interpret all data obtained. The combination of 2 mL HNO₃ and 1 mL H₂O₂ volumes, 0.1 g sample weight, 1400 W power and 5 min digestion time was found to be the optimum parameters required to digest the shrimp paste samples. Tests with spiked samples presented good recoveries with relative standard deviations between 0.32% and 5.35%.     

In-Band Device to Device (D2D) Communication and Device Discovery: A Survey

Wireless Personal Communications - Device to device (D2D) communication is one of the potentials to achieve the established standards for 5G. It represents a direct communication between two...     

Adsorption of humic acid onto chitin and chitosan

The adsorption of humic acid onto chitin and chitosan has been investigated. The uptake of humic acid from aqueous solution was determined from changes in concentration, as measured by ultraviolet-visible spectroscopy. The decrease in humic acid removal was observed with the increase in pH. A significant uptake of humic acid on both chitin and chitosan was observed. The uptake of humid acid on chitosan was greater than that on chitin. Adsorption isothermal data could be interpreted by the Langmuir and Freundlich equation. Langmuir and Freundlich constants have been determined. The experimental data of the adsorption equilibrium from humic acid solutions correlate well with the Langmuir isotherm equation, as compared to the Freundlich isotherm equation. © 1998 John Wiley & Sons, Inc. J Appl Polym Sci 69: 2305–2310, 1998     

Efficient Hardware-Based Image Compression Schemes for Wireless Sensor Networks: A Survey

Multidimensional sensors, such as digital camera sensors in the visual sensor networks VSNs generate a huge amount of information compared with the scalar sensors in the wireless sensor networks WSNs. Processing and transmitting such data from low power sensor nodes is a challenging issue through their limited computational and restricted bandwidth requirements in a hardware constrained environment. Source coding can be used to reduce the size of vision data collected by the sensor nodes before sending it to its destination. With image compression, a more efficient method of processing and transmission can be obtained by removing the redundant information from the captured image raw data. In this paper, a survey of the main types of the conventional state of the art image compression standards such as JPEG and JPEG2000 is provided. A literature review of their advantages and shortcomings of the application of these algorithms in the VSN hardware environment is specified. Moreover, the main factors influencing the design of compression algorithms in the context of VSN are presented. The selected compression algorithm may have some hardware-oriented properties such as; simplicity in coding, low memory need, low computational load, and high-compression rate. In this survey paper, an energy efficient hardware based image compression is highly requested to counter the severe hardware constraints in the WSNs.     

Preparation and characterization of chitosan–zeolite composites

Double crosslinked chitosan–zeolite (CZ-2) and noncrosslinked chitosan–zeolite (CZ-0) composites were prepared and characterized by using Fourier transform infrared (FTIR) spectrometer, surface area analyzer, scanning electron microscope coupled with energy dispersive X-ray (SEM-EDX) spectrometer, thermogravimetric analyzer (TGA), X-ray diffraction analyzer (XRD) and carbon, hydrogen, nitrogen (CHN) analyzer. After crosslinking, CZ-2 showed a reduction in surface area and CHN content in comparison to chitosan, zeolite, and CZ-0. Crosslinking resulted in improved stability of CZ-2 in distilled water, acetic acid and NaOH as CZ-2 recorded the lowest percentage of swelling. XRD diffractograms confirmed the formation of composites as there was a marked difference in the peak intensity at 2θ = 19.8°. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012     

Multi-user Shared Access (MUSA) procedure for device discovery in D2D communication

Telecommunication Systems - In a wireless self-organizing and the network-assisted Device-to-Device (D2D) network, proximal Device Discovery (DD) plays a noteworthy role. It is the foundation for...     

Perturbation of cellular immune functions in cigarette smokers and protection by palm oil vitamin E supplementation

Background

Cigarette smoke contains free radicals and an have adverse effect to the immune system. Supplementation of palm oil vitamin E (palmvitee), is known has antioxidant properties is thought to be beneficial for system immune protection against free radicals activity. The objective of the study was to determine the effect of palmvitee supplementation on immune response in smokers.

Methods

This study involved a group of smokers and nonsmokers who received 200?mg/day palmvitee and placebo for the control group. Blood samples were taken at 0, 12 and 24?weeks of supplementation. Plasma tocopherol and tocotrienol were determined by HPLC, lymphocyte proliferation by lymphocyte transformation test (LTT) and enumeration of lymphocytes T and B cells by flow cytometry. Statistical analysis was performed by Mann–Whitney U-test for non-parametric data distribution and correlation among the variables was examined by Spearman.

Results

Plasma tocopherol and tocotrienol were increased in vitamin E supplemented group as compared to placebo group. Urine cotinine levels and serum α₁-antitrypsin were significantly higher in smokers compared to nonsmokers. Lymphocyte proliferation induced by PHA showed an increasing trend with palmvitee supplementation in both smokers and nonsmokers. Natural killer cells were decreased; CD4⁺ cells and B cells were increased in smokers compared to nonsmokers but were unaffected with vitamin E supplementation except in the percentage of B cells which were increased in nonsmokers supplemented palmvitee compared to placebo. CD4⁺/CD8⁺ ratio was increased in smokers compared to nonsmokers. The high TWBC count observed in smokers correlated with the increased CD4⁺ and B cells.

Conclusions

Smoking caused alterations in certain immune parameters and palmvitee supplementation tended to cause an increase in lymphocytes transformation test but had no effect on CD3⁺, CD4⁺, CD8⁺, NK cells and B cells except B cells percentage in nonsmokers.