Effects of breed and age at slaughter on degradation of muscle lipids during processing of dry‐cured hams

Hams from Landrace, Duroc and Hampshire pigs slaughtered at ages 6, 7.5 and 9 months were processed to generate Norwegian Parma‐style hams. Lipid contents and the compositions of fatty acid classes (ΣSFA, ΣMUFA, ΣPUFA) within neutral lipids, phospholipids and free fatty acids were determined. Small differences in lipid degradation and composition of the classes were revealed. However, significant sensory differences related to lipids were observed. Breed was more important than age. Dry‐cured Hampshire hams gave a more intense mature odour that may be associated with higher overall lipid degradation. Unexpectedly, these hams also demonstrated high juiciness and tenderness, which could be related to the melting characteristics of the fat. Dry‐cured Duroc hams showed a higher susceptibility towards rancidity, presumably associated with preferential oxidation of n‐6 fatty acids relative to C18:1 n‐9. Dry‐cured Landrace hams showed the lowest juiciness and tenderness, likely due to their lower fat content (marbling).     

Multicellular Tumor Spheroids (MCTS) as a 3D In Vitro Evaluation Tool of Nanoparticles

Multicellular tumor spheroid models (MCTS) are often coined as 3D in vitro models that can mimic the microenvironment of tissues. MCTS have gained increasing interest in the nano‐biotechnology field as they can provide easily accessible information on the performance of nanoparticles without using animal models. Considering that many countries have put restrictions on animals testing, which will only tighten in the future as seen by the recent developments in the Netherlands, 3D models will become an even more valuable tool. Here, an overview on MCTS is provided, focusing on their use in cancer research as most nanoparticles are tested in MCTS for treatment of primary tumors. Thereafter, various types of nanoparticles—from self‐assembled block copolymers to inorganic nanoparticles, are discussed. A range of physicochemical parameters including the size, shape, surface chemistry, ligands attachment, stability, and stiffness are found to influence nanoparticles in MCTS. Some of these studies are complemented by animal studies confirming that lessons from MCTS can in part predict the behaviour in vivo. In summary, MCTS are suitable models to gain additional information on nanoparticles. While not being able to replace in vivo studies, they can bridge the gap between traditional 2D in vitro studies and in vivo models.     

Automated and Continuous Production of Microstructured Metallic Plates via Cold Embossing

Many critical issues need to be addressed when microstructured reactors are manufactured in large unit volumes. The most crucial of these are cost, ease of production, and reliability. The lack of breakthrough manufacturing technology to provide high‐efficiency, low‐cost, high‐precision plates is a hindrance to the early market implementation of systems requiring metallic microstructured plates. This contribution focuses on the development and optimization of a combined embossing and bending tool for the quick and continuous manufacture of easily machined plates.     

The influence of hydroelectric power generation on the body composition of juvenile Atlantic salmon

The fat and protein content, and thus energy resources of young (parr) Atlantic salmon (Salmo salar) were examined in a temperate river. Juvenile salmon exhibited distinct seasonal patterns of energy storage and use. The lowest levels of energy were found towards the end of winter (mean mass specific energy about 4.3 kJ·g^?1). There was a rapid accumulation of fat in spring/early summer in spite of low‐water temperatures (highest mean content of mass specific energy of about 5.0 kJ·g^?1 in June). By early autumn (August) there was a rapid depletion of fat and energy resources. The decline in specific energy during late summer was of the same magnitude as the corresponding winter decline. Furthermore, total fat and protein content, and thus energy resources of juveniles below a hydroelectric power plant (hydrostation (HS)) differed from those at a (lower) reference station (RS). The younger fish exhibited higher gains, but also larger losses of energy resources compared to corresponding values at the RS. Specifically during the summer, young of year (YOY) had 31% lower total energy content at the regulated site (mean 1.27 kJ per individual) than the RS (mean 1.84 kJ). Higher mortality may occur at the site most affected by the hydroelectric plant, where energy resources on average were higher, but where depletion was also most severe. Copyright © 2006 John Wiley & Sons, Ltd.     

Delamination vs matrix cracking in layered composites subjected to transverse loading

An algorithm is presented to compute the distribution of the strain energy release rate along the crack front of a penny-shaped delamination in a layered orthotropic body. The method applies a finite element recently proposed for three-dimensional analysis of layered orthotropic circular plates. The algorithm is economical even though it treats a full three-dimensional state of stress. The method requires only a single virtual crack extension to accurately compute the strain energy release rate at a point along the crack front. The method is applied to the study of delamination crack growth in a nine layer cross-ply laminate. The variation of strain energy release rate, G, along the crack front, is determined. The significance of the plate aspect ratio, as well as length scale, on the fracture process is studied. The establishment of a loading case where a distributed transverse compressive loading causes delamination growth is given.     

OWL2Vec*: embedding of OWL ontologies

Machine Learning - Semantic embedding of knowledge graphs has been widely studied and used for prediction and statistical analysis tasks across various domains such as Natural Language Processing...     

Exploring the Biomaterial-Induced Secretome: Physical Bone Substitute Characteristics Influence the Cytokine Expression of Macrophages

In addition to their chemical composition various physical properties of synthetic bone substitute materials have been shown to influence their regenerative potential and to influence the expression of cytokines produced by monocytes, the key cell-type responsible for tissue reaction to biomaterials in vivo. In the present study both the regenerative potential and the inflammatory response to five bone substitute materials all based on β-tricalcium phosphate (β-TCP), but which differed in their physical characteristics (i.e., granule size, granule shape and porosity) were analyzed for their effects on monocyte cytokine expression. To determine the effects of the physical characteristics of the different materials, the proliferation of primary human osteoblasts growing on the materials was analyzed. To determine the immunogenic effects of the different materials on human peripheral blood monocytes, cells cultured on the materials were evaluated for the expression of 14 pro- and anti-inflammatory cytokines, i.e., IL-6, IL-10, IL-1β, VEGF, RANTES, IL-12p40, I-CAM, IL-4, V-CAM, TNF-α, GM-CSF, MIP-1α, Il-8 and MCP-1 using a Bio-Plex^® Multiplex System. The granular shape of bone substitutes showed a significant influence on the osteoblast proliferation. Moreover, smaller pore sizes, round granular shape and larger granule size increased the expression of GM-CSF, RANTES, IL-10 and IL-12 by monocytes, while polygonal shape and the larger pore sizes increased the expression of V-CAM. The physical characteristics of a bone biomaterial can influence the proliferation rate of osteoblasts and has an influence on the cytokine gene expression of monocytes in vitro. These results indicate that the physical structure of a biomaterial has a significant effect of how cells interact with the material. Thus, specific characteristics of a material may strongly affect the regenerative potential in vivo.