Quantitative Proteomic Approach Reveals Altered Metabolic Pathways in Response to the Inhibition of Lysine Deacetylases in A549 Cells under Normoxia and Hypoxia

Growing evidence is showing that acetylation plays an essential role in cancer, but studies on the impact of KDAC inhibition (KDACi) on the metabolic profile are still in their infancy. Here, we analyzed, by using an iTRAQ-based quantitative proteomics approach, the changes in the proteome of KRAS-mutated non-small cell lung cancer (NSCLC) A549 cells in response to trichostatin-A (TSA) and nicotinamide (NAM) under normoxia and hypoxia. Part of this response was further validated by molecular and biochemical analyses and correlated with the proliferation rates, apoptotic cell death, and activation of ROS scavenging mechanisms in opposition to the ROS production. Despite the differences among the KDAC inhibitors, up-regulation of glycolysis, TCA cycle, oxidative phosphorylation and fatty acid synthesis emerged as a common metabolic response underlying KDACi. We also observed that some of the KDACi effects at metabolic levels are enhanced under hypoxia. Furthermore, we used a drug repositioning machine learning approach to list candidate metabolic therapeutic agents for KRAS mutated NSCLC. Together, these results allow us to better understand the metabolic regulations underlying KDACi in NSCLC, taking into account the microenvironment of tumors related to hypoxia, and bring new insights for the future rational design of new therapies.     

Densification involved in the UV-based photosensitivity of silicaglasses and optical fibers

A comprehensive survey of photosensitivity in silica glasses and optical fiber is reviewed. Recent work on understanding the mechanisms contributing to germanium or aluminum doped fiber photosensitivity is discussed within the framework of photoelastic densification models     

Material dispersion evaluation in a fluoride glass

We report for the first time experimental evaluation of the material dispersion in a fluoride glass. Very interesting features are observed as the wavelength of zero material dispersion is around 1.7 ?m and the disperison is about only 45 ps/nm × km at a 4 ?m wavelength.     

Viscosity and optical measurement on fluoride glasses

Scattering loss and viscosity against temperature measurements have been carried out on fluoride glasses. The results indicate for the first time a ?-4 behaviour for scattering loss in the 0.45?0.75 ?m wavelength range. Activation energies for viscous flow were found to be similar for both ZrF4? and HfF4?based glasses.     

Laser cross-section and quantum yield of Er3+ at 2.7μm in a ZrF4-based fluoride glass

Spectroscopic determination of laser cross-section and quantum efficiency of the Er³⁺ laser transition at 2.7 μm are reported for the first time for a fluoride glass of the ZBLAN type. Comparisons with crystal values and other glass compositions are given. Emission spectra of Er³⁺ at 2.7 μm are also presented for the first time     

Multimodal person discovery in broadcast TV: lessons learned from MediaEval 2015

Multimedia Tools and Applications - We describe the “Multimodal Person Discovery in Broadcast TV” task of MediaEval 2015 benchmarking initiative. Participants were asked to return the...     

Erbium-doped fluorozirconate single-mode fibre lasing at 2.71 mu m

Continuous-wave lasing has been obtained at 2.714 mu m in a fluorozirconate single-mode fibre when pumping at 476.5, 501.7 or 647.1 nm. A threshold pump power of 7 mW, an output laser power of 250 mu W and a linewidth less than 2 nm were measured.<>     

Tunable green upconversion erbium fibre laser

Fifty mW of green power and slope efficiency against launched pump power of 15% have been obtained at room temperature in an erbium doped fluorozirconate fibre pumped at 0.97 mu m. Lasing tunability domain, pump wavelength tunability and the role of colasing at 1.55 mu m are described.<>     

Naming multi-modal clusters to identify persons in TV broadcast

Persons’ identification in TV broadcast is one of the main tools to index this type of videos. The classical way is to use biometric face and speaker models, but, to cover a decent number of persons, costly annotations are needed. Over the recent years, several works have proposed to use other sources of names for identifying people, such as pronounced names and written names. The main idea is to form face/speaker clusters based on their similarities and to propagate these names onto clusters. In this paper, we propose a method to take advantage of written names during the diarization process, in order to both name clusters and prevent the fusion of two clusters named differently. First, we extract written names with the LOOV tool (Poignant et al. 2012); these names are associated to their co-occurring speaker turns / face tracks. Simultaneously, we build a multi-modal matrix of distances between speaker turns and face tracks. Then agglomerative clustering is performed on this matrix with the constraint to avoid merging clusters associated to different names. We also integrate the prediction of few biometric models (anchors, some journalists) to directly identify speaker turns / face tracks before the clustering process. Our approach was evaluated on the REPERE corpus and reached an F-measure of 68.2 % for speaker identification and 60.2 % for face identification. Adding few biometric models improves results and leads to 82.4 % and 65.6 % for speaker and face identity respectively. By comparison, a mono-modal, supervised person identification system with 706 speaker models trained on matching development data and additional TV and radio data provides 67.8 % F-measure, while 908 face models provide only 30.5 % F-measure.     

Ytterbium-doped fluoride fibre laser operating at 1.02 mu m

Efficient laser emission has been observed around 1.02 mu m in a monomode ZBLAN fibre doped with ytterbium. Various concentrations, fibre lengths and cavity configurations have been studied. Differences between silica and ZBLAN are highlighted.<>