Crystal structure of tRNA-guanine transglycosylase: RNA modification by base exchange

tRNA-guanine transglycosylases (TGT) are enzymes involved in the modification of the anticodon of tRNAs specific for Asn, Asp, His and Tyr, leading to the replacement of guanine-34 at the wobble position by the hypermodified base queuine. In prokaryotes TGT catalyzes the exchange of guanine-34 with the queuine (.)precursor 7-aminomethyl-7-deazaguanine (preQ1). The crystal structure of TGT from Zymomonas mobilis was solved by multiple isomorphous replacement and refined to a crystallographic R-factor of 19% at 1.85 angstrom resolution. The structure consists of an irregular (beta/alpha)8-barrel with a tightly attached C-terminal zinc-containing subdomain. The packing of the subdomain against the barrel is mediated by an alpha-helix, located close to the C-terminus, which displaces the eighth helix of the barrel. The structure of TGT in complex with preQ1 suggests a binding mode for tRNA where the phosphate backbone interacts with the zinc subdomain and the U33G34U35 sequence is recognized by the barrel. This model for tRNA binding is consistent with a base exchange mechanism involving a covalent tRNA-enzyme intermediate. This structure is the first example of a (beta/alpha)-barrel protein interacting specifically with a nucleic acid.     

Laplace spectra as fingerprints for image recognition

In the area of image retrieval from data bases and for copyright protection of large image collections there is a growing demand for unique but easily computable fingerprints for images. These fingerprints can be used to quickly identify every image within a larger set of possibly similar images. This paper introduces a novel method to automatically obtain such fingerprints from an image. It is based on a reinterpretation of an image as a Riemannian manifold. This representation is feasible for gray value images and color images. We discuss the use of the spectrum of eigenvalues of different variants of the Laplace operator as a fingerprint and show the usability of this approach in several use cases. Contrary to existing works in this area we do not only use the discrete Laplacian, but also with a particular emphasis the underlying continuous operator. This allows better results in comparing the resulting spectra and deeper insights in the problems arising. We show how the well known discrete Laplacian is related to the continuous Laplace-Beltrami operator. Furthermore, we introduce the new concept of solid height functions to overcome some potential limitations of the method.     

Contributions to the Ohmic Drop in the Electrolysis of ZnCl2 in a Molten Chloride Electrolyte

The electrowinning of zinc from zinc chloride with a molten chloride electrolyte was investigated. The electrolysis of zinc chloride shows ohmic limitations. The energy consumption is to a large extent determined by the anodic reaction, the evolution of chlorine. The chlorine gas plume was visualised in a see-through furnace and images were analysed to determine the plume angle. This parameter serves as an input parameter for a model to determine the ohmic contributions of the electrolyte, the plume and a layer of chlorine bubbles sticking to the anode, as well as the plume velocity. The results indicate that the major contribution (60%) to the ohmic drop is due to a stagnant layer of bubbles growing and sticking to the anode, thereby decreasing the effective anode area by coverage. The plume velocity influences coverage characteristics to some extent, which influences the ohmic drop.     

A formally specified ontology management API as a registry for ubiquitous computing systems

Recently, several standards have emerged for ontology markup languages that can be used to formalize all kinds of knowledge. However, there are no widely accepted standards yet that define APIs to manage ontological data. Processing ontological information still suffers from the heterogeneity imposed by the plethora of available ontology management systems. Moreover, ubiquitous computing environments usually comprise software components written in a variety of different programming languages, which makes it particularly difficult to establish a common ontology management API with programming language agnostic semantics. We implemented an ontological Knowledge Base Server, which can expose the functionality of arbitrary off-the-shelf ontology management systems via a formally specified and well defined API. A case study was carried out in order to demonstrate the feasibility of our approach to use a formally specified ontology management API to implement a registry for ubiquitous computing systems.     

Personality and Emotion: Test of Gray's Personality Theory by Means of an fMRI Study.

Although it is known that there are fundamental personality differences in the behavioral responses to emotional stimuli, traits have scarcely been investigated in this context by means of functional imaging studies. To maximize the variance with respect to personality, the authors tested 12 control subjects and 12 subjects who had sadomasochistic experiences with respect to the relationship between J. A. Gray's (1970) personality dimensions, the behavioral approach system (BAS) and the behavioral inhibition system (BIS), and brain activity in regions of interest. The BIS was associated with activity in numerous brain areas in response to fear, disgust, and erotic visual stimuli, whereas few associations could he detected between the BAS and brain activity in response to disgust and erotic stimuli. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

27-GHz bandwidth high-speed monolithic integrated optoelectronicphotoreceiver consisting of a waveguide fed photodiode and anInAlAs/InGaAs-HFET traveling wave amplifier

A monolithic integrated photoreceiver for 1.55-μm wavelength has been designed for operation in a 20-Gb/s synchronous digital hierarchy system (SDH/SONET), based on a new integration concept. The optoelectronic integrated circuit (OEIC) receiver combines a waveguide-integrated PIN-photodiode and a traveling wave amplifier in coplanar waveguide layout with four InAlAs/InGaAs/InP-HFETs (0.7-μm gate length). The receiver demonstrates a bandwidth of 27 GHz with a low frequency transimpedance of 40 dBΩ. This is, to our knowledge, the highest bandwidth ever reported for a monolithic integrated photoreceiver on InP. Furthermore, a receiver sensitivity of -12 dBm in the fiber (20 Gb/s, BER=10^-9) and an overall optical input dynamic range of 27 dB is achieved. Optical time domain multiplex (TDM) system experiments of the receiver packaged in a module show an excellently shaped eye pattern for 20 Gb/s and an overall sensitivity of -30.5 dBm (BER=10^-9) [including erbium doped fiber amplifiers (EDFA)]