Pull-Down of Metalloproteins in Their Native States by Using Desthiobiotin-Based Probes

One-third of all proteins are estimated to require metals for structural stability and/or catalytic activity. Desthiobiotin probes containing metal binding groups can be used to capture metalloproteins with exposed active-site metals under mild conditions so as to prevent changes in metallation state. The proof-of-concept was demonstrated with carbonic anhydrase (CA), an open active site, Zn²⁺-containing protein. CA was targeted by using sulfonamide derivatives. Linkers of various lengths and structures were screened to determine the optimal structure for capture of the native protein. The optimized probes could selectively pull down CA from red blood cell lysate and other protein mixtures. Pull-down of differently metallated CAs was also investigated.     

Development of structurally modified OER catalysts with enhanced performance and longevity for PEM-based electrolytic air dehumidification

PEM-based electrolytic air dehumidification is innovative in dehumidification that requires high precision and small space due to its high efficiency, compactness, and cleanness. However, the system dehumidification performance and durability are limited by using commercial Anatase-IrO₂ catalysts. In this study, two types of structurally modified OER catalyst materials, ATO-IrO₂ and ND-MnO₂-IrO₂, are developed to improve the performance of the system. System experiments showed that, compared to the commercial catalysts, the use of ATO-IrO₂ and ND-MnO₂-IrO₂ as the anode catalyst can improve the dehumidification performance by 45% and 20%, respectively. Furthermore, in 50-h accelerated aging tests, the attenuation rates of the ATO-IrO₂ and ND-MnO₂-IrO₂ systems are 3% and 8% respectively, which are far lower than the 35% attenuation of commercial catalyst. The results indicate that, as catalysts with a classic core-shell structure, ATO-IrO₂ and ND-MnO₂-IrO₂ still have a significant impact on improving the performance of the electrolytic dehumidification systems.     

渣油胶质和沥青质在分散型催化剂作用下的临氢热反应行为

采用四组分法从辽河减压渣油中分离出胶质、沥青质，分别考察了它们在分散型催化剂作用下的临氢热反应行为。结果表明，胶质、沥青质一方面要裂化生成较轻的产物；另一方面也要发生缩合反应生成较重的反应产物以及甲苯不溶物。渣油在临氢热反应过程中，沥青质是生焦的主要来源，其次才是胶质。比较了热反应生成的沥青质与原生沥青质之间以及热反应生成的胶质与原生胶质之间化学组成的区别，结果表明，反应生成的沥青质和胶质较原生的沥青质和胶质分子结构缩合程度高，并且反应苛刻度越高，缩合程度也越高。     

催化精馏合成N-异丙基苯胺

在Joback基团贡献法估算反应的G ibbs函数的基础上,得到了异丙醇与苯胺合成N-异丙基苯胺的平衡常数与反应温度的关系式。研究了回流比、异丙醇与苯胺的摩尔比、塔釜操作温度、苯胺进料口位置距塔顶的距离对异丙醇与苯胺催化精馏合成N-异丙基苯胺的影响,确定了适宜的工艺条件:回流比2.5~3.5、异丙醇与苯胺的摩尔比1.5、塔釜操作温度223℃、苯胺进料口位置距塔顶300~400mm。在优化条件下,苯胺的转化率可达到99%,N-异丙基苯胺的选择性可达到99.5%,N-异丙基苯胺的质量分数可稳定在98.8%以上。     

氦氖激光对人胚腱细胞生长分泌的影响

目的 阐明氦氖激光促进人胚腱细胞增殖及分泌胶原的机制。方法 将传代培养的人胚腱细胞分为对照组、照射1日组、照射3日组及照射5日组。在氦氖激光照射的不同时相点，分别测定细胞DNA含量，cAMP水平及胶原分泌量。结果 在氦氖激光作用早期（1－3日），细胞cAMP水平与DNA合成均呈明显升高（P＜0.05)，细胞内信使物质合成及细胞增殖加快，但胶原分泌无变化，而在氦氖激光作用晚期（3－5日），当cAMP水平及DNA合成都不再改变时，腱细胞分泌胶原量显著增加（P＜0.01)。结论 氦氖激光可能通过作用于cAMP蛋白激酶A信号转导系统，从而调控人胚腱细胞增殖及胶原分泌。提示临床应用氛氖激光促进肌腱愈合宜从早期开始，并且需要照射足够长的时间。     

提高自蔓延陶瓷内衬钢管耐蚀性研究的进展

广泛应用于石油，化工，能源等领域的钢管，除了要有高强度外，还要有良好的耐蚀性。自蔓延高温合成技术是制备耐蚀钢管的新技术，具有工艺简单，成本低和产品耐蚀性能好等特点。介绍了自蔓延高温合成陶瓷内衬钢管的原理和提高陶瓷内衬钢管耐蚀性的措施，展望了耐蚀陶瓷内衬钢管在工业生产上的应用前景。     

片状陶瓷材料高低温电性能参数的自动检测

由进口电性能仪配上自制的高温系统，计算机自动采集数据，配置成一套高低温电性参数测定装置，该装置的特点是，升温快，炉体小巧，炉芯可更换盛液氮装置，屏蔽良好，等温区长，温工及参数测量精确可靠。     

水包油型多彩涂料的生产及施工

介绍了水包油型多彩涂料的组成、各组份的作用、生产工艺路线和工艺参数的选择。对多彩涂料的施工机具和方法作了简单介绍。     

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本文提出了油井稳定生产状态下的脉冲试井数学模型,利用时间和空间叠加原理求得其解析解。并用非线性回归分析方法来进行脉冲试井分析。     

高压热处理对氧沉淀低温形核的影响

研究了外加高压(1GPa)下对450℃热处理硅片中氧沉淀行为的影响.透射电镜观察表明相对于大气压下热处理的样品,高压处理的样品会产生密度更高、尺寸更小的氧沉淀,表明高压有利于小直径氧沉淀的生成.电学性能测试表明,高压下处理的样品其热施主生成浓度和生成速率远远高于常压下处理的样品,这表明热施主与低温热处理过程中生成的高浓度氧沉淀核心有密切的关系.