A 1.2-V Highly Linear Balanced Noise-Cancelling LNA in 0.13-μm CMOS

In this paper, a current-to-voltage combiner is proposed to realize a highly linear, balanced noise-cancelling low-noise amplifier (LNA) capable of low-voltage operation. The current-to-voltage combiner, implemented in the load of the amplifier, converts the output currents of the parallel common-gate (CG) and common-source (CS) stages of the LNA to voltages, equalizes the amplitudes of the voltages, and combines the voltages to a single output voltage. Since only a CS stage and passive components are employed to cancel the noise and distortion due to the CG input impedance matching circuit, high linearity is achieved in spite of the low supply voltage of 1.2 V. The LNA achieves a noise figure (NF) of 3.0 dB at 2.1 GHz with an input-referred third-order intercept point (IIP3) of +10.5 dBm while consuming 10.5 mA from a 1.2-V supply. The amplifier is fabricated in 0.13-mum CMOS process.     

Solution structure of nodularin. An inhibitor of serine/threonine-specific protein phosphatases

The three-dimensional solution structure of nodularin was studied by NMR and molecular dynamics simulations. The conformation in water was determined from the distance and dihedral data by distance geometry and refined by iterative relaxation matrix analysis. The cyclic backbone adopts a well defined conformation but the remote parts of the side chains of arginine as well as the amino acid derivative Adda have a large spatial dispersion. For the unusual amino acids the partial charges were calculated and nodularin was subjected to molecular dynamic simulations in water. A good agreement was found between experimental and computational data with hydrogen bonds, solvent accessibility, molecular motion, and conformational exchange. The three-dimensional structure resembles very closely that of microcystin-LR in the chemically equivalent segment. Therefore, it is expected that the binding of both microcystins and nodularins to serine/threonine-specific protein phosphatases is similar on an atomic level.     

The mitochondrial toxin produced by Streptomyces griseus strains isolated from an indoor environment is valinomycin

Actinomycete isolates from indoor air and dust in water-damaged schools and children's day care centers were tested for toxicity by using boar spermatozoa as an indicator. Toxicity was detected in extracts of four strains which caused a loss of sperm motility, and the 50% effective concentrations (EC50) were 10 to 63 ng (dry weight) ml of extended boar semen-1. The four strains were identified as Streptomyces griseus strains by 16S ribosomal DNA and chemotaxonomic methods. The four S. griseus strains had similar effects on sperm cells, including loss of motility and swelling of mitochondria, but we observed no loss of plasma membrane integrity or depletion of cellular ATP. None of the effects was observed with sperm cells exposed to extracts of other indoor actinomycete isolates at concentrations of >/=5,000 to 72,000 ng ml-1. The toxin was purified from all four strains and was identified as a dodecadepsipeptide, and the fragmentation pattern obtained by tandem mass spectrometry was identical to that of valinomycin. Commercial valinomycin had effects in sperm cells that were identical to the effects of the four indoor isolates of S. griseus. The EC50 of purified toxin from the S. griseus strains were 1 to 3 ng ml of extended boar semen-1, and the EC50 of commercial valinomycin was 2 ng ml of extended boar semen-1. To our knowledge, this is the first report of the presence of ionophoric toxin producers in an indoor environment and the first report of valinomycin-producing strains identified as S. griseus.     

Reed canary grass transportation costs – Reducing costs and increasing feasible transportation distances

Reed canary grass is used in several heat and power plants in Finland and it is estimated that about 70–80 plants could utilise reed canary grass as a co-firing fuel. Long-distance transport of reed canary grass forms a significant share of the production and delivery costs. Reed canary grass can be transported in bales or as loose matter. The density of the matter and shape of the bales influence the load-size and the cost. At present it is impossible to obtain the full load-bearing capacity of a lorry even with bales, and with light bulk matter the obtainable load is less than one-third of the load-bearing capacity of a lorry. By using Orkel local-baler it has been possible to obtain the largest load-sizes, but the total economy of the chain is not very good because the baling and chopping of bales increase the total costs. If a full load-bearing capacity of a lorry (30–40 tonne) would be obtained, the transportation costs would be reduced significantly compared to the present situation. Second alternative would be to mix reed canary grass with wood chips or peat before long-distance transport. At the moment feasible transportation distances are relatively short. With briquettes the cargo space could be used more efficiently and the load-size of the transport could be increased.     

Cancellation of second-order intermodulation distortion and enhancement of IIP2 in common-source and common-emitter RF transconductors

In this paper, a biasing technique for cancelling second-order intermodulation (IM2) distortion and enhancing second-order intercept point (IIP2) in common-source and common-emitter RF transconductors is presented. The proposed circuit can be utilized as an RF input transconductor in double-balanced downconversion mixers. By applying the presented technique, the achievable IIP2 of the mixer is limited by the linearity of the switching devices, component mismatches, and offsets. The proposed circuit has properties similar to the conventional differential pair transconductor in that it ideally displays no IM2 distortion. However, the presented circuit is more suitable for operation at low supply voltages because it has only one device stacked between the transconductor input and output. In the conventional differential pair, two devices consume the voltage headroom. The noise performance of the proposed transconductor is similar to the noise performance of the traditional common-source (emitter) and differential pair transconductors at given bias and device dimensions. On the other hand, the third-order intercept point (IIP3) of the presented transconductor is slightly higher than the IIP3 of the differential pair transconductor at given bias. Finally, the proposed circuit can also be employed as a current mirror, the ratio of which is very insensitive to the voltage swings at the gate or base of the current mirrored transistor.     

A 1.2-V RF front-end with on-chip VCO for PCS 1900 direct conversion receiver in 0.13-/spl mu/m CMOS

In this paper, a 1.2-V RF front-end realized for the personal communications services (PCS) direct conversion receiver is presented. The RF front-end comprises a low-noise amplifier (LNA), quadrature mixers, and active RC low-pass filters with gain control. Quadrature local oscillator (LO) signals are generated on chip by a double-frequency voltage-controlled oscillator (VCO) and frequency divider. A current-mode interface between the downconversion mixer output and analog baseband input together with a dynamic matching technique simultaneously improves the mixer linearity, allows the reduction of flicker noise due to the mixer switches, and minimizes the noise contribution of the analog baseband. The dynamic matching technique is employed to suppress the flicker noise of the common-mode feedback (CMFB) circuit utilized at the mixer output, which otherwise would dominate the low-frequency noise of the mixer. Various low-voltage circuit techniques are employed to enhance both the mixer second- and third-order linearity, and to lower the flicker noise. The RF front-end is fabricated in a 0.13-/spl mu/m CMOS process utilizing only standard process options. The RF front-end achieves a voltage gain of 50 dB, noise figure of 3.9 dB when integrated from 100 Hz to 135 kHz, IIP3 of -9 dBm, and at least IIP2 of +30dBm without calibration. The 4-GHz VCO meets the PCS 1900 phase noise specifications and has a phase noise of -132dBc/Hz at 3-MHz offset.     

Screening for biohydrogen production by cyanobacteria isolated from the Baltic Sea and Finnish lakes

Cyanobacteria are the only bacteria capable of performing oxygenic photosynthesis in which they harness solar energy and convert it into chemical energy stored in carbohydrates. Under specific conditions, cyanobacteria can use solar energy to produce also molecular hydrogen.     

Long-term treatment of ulcerative colitis with ciprofloxacin: a prospective, double-blind, placebo-controlled study

BACKGROUND & AIMS: Although bacterial bowel flora may be one of the contributing factors in the pathogenesis of chronic mucosal inflammation, antibiotic treatment has no established role in ulcerative colitis. The aim of the study was to evaluate the role of ciprofloxacin in the induction and maintenance of remission in ulcerative colitis in patients responding poorly to conventional therapy with steroids and mesalamine. METHODS: Ciprofloxacin (n = 38; 500-750 mg twice a day) or placebo (n = 45) was administered for 6 months in a double-blind, randomized study with a high but decreasing dose of prednisone and maintenance treatment with mesalamine including follow-up for the next 6 months. Clinical assessment and colonoscopic evaluation were performed at 0, 3, 6, and 12 months. Treatment failure, the primary end point, was defined as both symptomatic and endoscopic failure to respond. RESULTS: During the first 6 months, the treatment-failure rate was 21% in the ciprofloxacin-treated group and 44% in the placebo group (P = 0.02). Endoscopic and histological findings were used as secondary end points and showed better results in the ciprofloxacin group at 3 months but not at 6 months. CONCLUSIONS: Addition of a 6-month ciprofloxacin treatment for ulcerative colitis improved the results of conventional therapy with mesalamine and prednisone.     

Extended H2 photoproduction by N2-fixing cyanobacteria immobilized in thin alginate films

Screening of the University of Helsinki Culture Collection for naturally good H₂ producing cyanobacteria recently revealed several promising strains. One of the superior strains is Calothrix 336/3, an N₂-fixing heterocystous filamentous cyanobacterium. Making use of an important feature of the Calothrix 336/3 cells to adhere to the substrate, we applied an immobilization technique to improve H₂ production capacity of this strain. We examined the basic properties of immobilization in Ca²⁺-alginate films in response to the production of H₂ of the Calothrix 336/3 strain and as reference strains we used a model organism Anabaena PCC 7120 and its uptake hydrogenase mutant, ΔhupL, that allow us to compare the responses of different strains to alginate entrapment. Immobilization of the Calothrix 336/3 and ΔhupL mutant cells in Ca²⁺-alginate resulted in prolonged H₂ production over several cycles. Immobilization of the Calothrix 336/3 cells was most successful and production of H₂ could be measured even after 40 days after immobilization.