Nitrogen plasma source ion implantation of AISI S1 tool steel

Significant economic savings can be achieved by improving the wear lifetime of precision manufacturing tools through nitrogen-ion implantation. This near-ambient temperature, surface modification process preserves dimensional integrity and surface finish while eliminating delamination problems that are often associated with overlay coatings. Conventional ion implantation is a line-of-sight process which requires elaborate manipulation and masking to uniformly implant components of complex shapes. A recently developed process, plasma source ion implantation (PSII), circumvents this line-of-sight restriction and makes ion implantation more attractive economically. In this article, the effects of PSII of nitrogen at a target bias of 50 kV, to a dose of 0.3 × 10¹⁸ atoms/cm² on the surface microstructure and mechanicalproperties of AISI S1 tool steel are presented.     

Scheduling a dynamic job shop production system with sequence-dependent setups: An experimental study

This paper presents the salient aspects of a simulation-based experimental study of scheduling rules for scheduling a dynamic job shop in which the setup times are sequence dependent. A discrete event simulation model of the job shop system is developed for the purpose of experimentation. Seven scheduling rules from the literature are incorporated in the simulation model. Five new setup-oriented scheduling rules are proposed and implemented. Simulation experiments have been conducted under various experimental conditions characterized by factors such as shop load, setup time ratios and due date tightness. The results indicate that setup-oriented rules provide better performance than ordinary rules. The difference in performance between these two groups of rules increases with increase in shop load and setup time ratio. One of the proposed rules performs better for mean flow time and mean tardiness measures.     

Simulation modelling and analysis of part and tool flow control decisions in a flexible manufacturing system

This paper presents the details of a simulation study carried out for analyzing the impact of scheduling rules that control part launching and tool request selection decisions of a flexible manufacturing system (FMS) operating under tool movement along with part movement policy. Two different scenarios have been investigated with respect to the operation of FMS. In scenario 1, the facilities such as machines, tool transporter and part transporter are assumed to be continuously available without breakdowns, whereas in scenario 2, these facilities are prone to failures. For each of these scenarios, a discrete-event simulation model is developed for the purpose of experimentation. A number of scheduling rules are incorporated in the simulation models for the part launching and tool request selection decisions. The performance measures evaluated are mean flow time, mean tardiness, mean waiting time for tool and percentage of tardy parts. The results obtained through the simulation have been statistically analyzed. The best possible scheduling rule combinations for part launching and tool request selection have been identified for the chosen FMS.     

A study on the effects of using short utterance length development data in the design of GPLDA speaker verification systems

This paper studies the performance degradation of Gaussian probabilistic linear discriminant analysis (GPLDA) speaker verification system, when only short-utterance data is used for speaker verification system development. Subsequently, a number of techniques, including utterance partitioning and source-normalised weighted linear discriminant analysis (SN-WLDA) projections are introduced to improve the speaker verification performance in such conditions. Experimental studies have found that when short utterance data is available for speaker verification development, GPLDA system overall achieves best performance with a lower number of universal background model (UBM) components. As a lower number of UBM components significantly reduces the computational complexity of speaker verification system, that is a useful observation. In limited session data conditions, we propose a simple utterance-partitioning technique, which when applied to the LDA-projected GPLDA system shows over 8% relative improvement on EER values over baseline system on NIST 2008 truncated 10–10 s conditions. We conjecture that this improvement arises from the apparent increase in the number of sessions arising from our partitioning technique and this helps to better model the GPLDA parameters. Further, partitioning SN-WLDA-projected GPLDA shows over 16% and 6% relative improvement on EER values over LDA-projected GPLDA systems respectively on NIST 2008 truncated 10–10 s interview-interview, and NIST 2010 truncated 10–10 s interview-interview and telephone-telephone conditions.     

Improving Speech Recognition Accuracy for Small Vocabulary Applications in Adverse Environments

The problem of improving the accuracy of small vocabulary isolated word speaker dependent speech recognition under adverse conditions such as factory environments is considered. A new approach to solving this problem, by using Output Probability Distributions (OPDs), is presented. OPDs improve the system performance by modelling inter-word relationships, something that a standard maximum likelihood (ML) technique fails to do. The system was tested using the TI46 database, corrupted with the NOISEX-92 database, as well as in a real-world factory environment, and achieved good results.     

Corrosion of Alloy Haynes 230 in High Temperature Supercritical Carbon Dioxide with Oxygen Impurity Additions

The corrosion of Ni-based alloy Haynes 230 in supercritical carbon dioxide at temperatures of 650 and 750 °C at a pressure of 20 MPa was investigated. In high-purity research grade CO₂, the corrosion performance of the alloy was excellent with a thin, uniform, protective chromium-rich oxide layer forming on the surface. Introduction of 10 and 100 ppm O₂ impurity in the CO₂ environment noticeably enhanced oxidation with evidence of oxide spallation and nodule formation. In these oxygen impurity added tests, increased oxidation led to subsurface voids due to the more rapid outward diffusion of chromium as well as intergranular alumina and chromia. The oxygen concentration at the inlet and the outlet of the autoclave was measured and used to support the results of characterization of the surface oxide to develop a more holistic understanding of the role of oxygen impurity on the corrosion process. In all cases, there some carbon was observed, which manifested as slightly higher concentration of chromium–carbide phase at the grain boundaries compared to the unexposed alloy.     

THZ531 Induces a State of BRCAness in Multiple Myeloma Cells: Synthetic Lethality with Combination Treatment of THZ 531 with DNA Repair Inhibitors

Multiple myeloma (MM) is a hematological disease marked by abnormal growth of B cells in bone marrow. Inherent chromosomal instability and DNA damage are major hallmarks of MM, which implicates an aberrant DNA repair mechanism. Studies have implicated a role for CDK12 in the control of expression of DNA damage response genes. In this study, we examined the effect of a small molecule inhibitor of CDK12–THZ531 on MM cells. Treatment of MM cells with THZ531 led to heightened cell death accompanied by an extensive effect on gene expression changes. In particular, we observed downregulation of genes involved in DNA repair pathways. With this insight, we extended our study to identify synthetic lethal mechanisms that could be exploited for the treatment of MM cells. Combination of THZ531 with either DNA-PK inhibitor (KU-0060648) or PARP inhibitor (Olaparib) led to synergistic cell death. In addition, combination treatment of THZ531 with Olaparib significantly reduced tumor burden in animal models. Our findings suggest that using a CDK12 inhibitor in combination with other DNA repair inhibitors may establish an effective therapeutic regimen to benefit myeloma patients.     

Environmental testing of high strength molding compounds

Tensile and short beam shear tests were performed on five high strength vinyl ester molding compounds after immersion in water at various temperatures. These tests were conducted in order to characterize the degradation of these properties with environmental exposure and to isolate problem areas which contribute to the degradation. Reductions of up to 70 percent were found for both tensile and short beam shear strength, when tested at high temperatures. This loss of properties is primarily a result of degradation of the glass/resin interface and is essentially irreversible.     

Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India

Pyrazinamide (PZA) has been in use for almost 50 years as a first-line drug for short-course chemotherapy against Mycobacterium tuberculosis. In this study, PCR mediated automated DNA sequencing is used to check the prevalence of PZA resistance among treatment failure cases of pulmonary tuberculosis. Out of 50 clinical isolates examined, 39 had mutations in the pncA gene that encodes Pyrazinamidase, an enzyme required to activate PZA. Of these, 31 (79.5%) were localized to three regions of pncA. We found two isolates with hitherto unreported mutation at amino acid 26 (Ala→Gly) of pncA.