Wavelength reuse scheme in a WDM unidirectional ring network usinga proper fibre grating add/drop multiplexer

An experimental optical WDM ring network is proposed. It uses a proper add/drop multiplexer based on a fibre grating filter. Both-way transmission using a wavelength reuse scheme is successfully demonstrated without any crosstalk     

Interaction of Alpha Synuclein and Microtubule Organization Is Linked to Impaired Neuritic Integrity in Parkinson’s Patient-Derived Neuronal Cells

Parkinson’s disease (PD) is neuropathologically characterized by the loss of dopaminergic neurons and the deposition of aggregated alpha synuclein (aSyn). Mounting evidence suggests that neuritic degeneration precedes neuronal loss in PD. A possible underlying mechanism could be the interference of aSyn with microtubule organization in the neuritic development, as implied by several studies using cell-free model systems. In this study, we investigate the impact of aSyn on microtubule organization in aSyn overexpressing H4 neuroglioma cells and midbrain dopaminergic neuronal cells (mDANs) generated from PD patient-derived human induced pluripotent stem cells (hiPSCs) carrying an aSyn gene duplication (SNCA^Dupl). An unbiased mass spectrometric analysis reveals a preferential binding of aggregated aSyn conformers to a number of microtubule elements. We confirm the interaction of aSyn with beta tubulin III in H4 and hiPSC-derived mDAN cell model systems, and demonstrate a remarkable redistribution of tubulin isoforms from the soluble to insoluble fraction, accompanied by a significantly increased insoluble aSyn level. Concordantly, SNCA^Dupl mDANs show impaired neuritic phenotypes characterized by perturbations in neurite initiation and outgrowth. In summary, our findings suggest a mechanistic pathway, through which aSyn aggregation interferes with microtubule organization and induces neurite impairments.     

Theoretical simulation and experimental investigation on a WDMsurvivable unidirectional open ring network using tunable channelselecting receivers

In this paper theoretical and experimental investigations on a wavelength-division-multiplexed (WDM) reconfigurable open ring network are reported. The theoretical study is focused on network component characteristics and ring network structure. These aspects will form the basis of numerical modeling. A powerful computer aided design software “COMSIS” is used for the simulation. The simulation takes into account the most important parameters: node structure, link losses, EDFA characteristics, optical powers of the channels, and signal wavelength arrangements. The power of a new channel added at each node and its wavelength with respect to those present before the node are two important parameters in the performance analysis of this open ring network. If the performance criterion is to receive the channels with high signal to noise ratios (SNR) and a narrow spread ΔSNR, the optimal length of this ring network can reach 366 km for 4 nodes and 565 km for 8 nodes. The experimental demonstrator is composed of 4 secondary nodes incorporating tunable channel selecting receivers (TCSR's) and erbium doped fiber amplifiers (EDFA's). The reconfiguration and the protection of the ring is computer controlled. Experimental results of a fully connected testbed demonstrator are also presented     

A General-Purpose 7 DOF Haptic Device: Applications Toward Robot-Assisted Surgery

A 7 DOF haptic device has been designed and developed with applications towards robot-assisted minimally invasive surgery. The device consists of four degrees of force feedback (X, Y, Z, and grasping) capability and seven degrees of position feedback capability. It has a closed kinematic chain with two halves (user interface and spatial mechanism) that connect together via a universal joint. The user interface contains four degrees of position feedback, namely, the roll, pitch, yaw, and linear motion of the hand and forearm. In addition, a grasping mechanism with two thimbles mounted at the end of the user interface provides force feedback to the fingers of the user. The spatial mechanism provides force feedback to the user interface through a universal joint located at the grasping mechanism. This paper presents the design and development of this haptic device. In addition, a kinematic and workspace analysis of the device has been completed to compute the position of the slave robot and end-effector tool. Friction estimation has been presented to enable a higher transparency of the haptic device. Finally, a simulation of needle insertion into soft tissue was developed to test the device.     

Model organism proteomics as a tool for the study of host–microbiome interactions

All organisms live in constant contact with the microbial world. In recent years it has become evident that these microbial communities are not only responsible for the development of certain diseases, but are also an indispensable factor for homeostasis. The inherent complexity of meta-organisms hampers a straightforward elucidation of the molecular processes regulating the interactions of the host and its microbiome, as well as the influence of exogenic factors, for example, nutrition. Modern approaches such as meta-proteomics are now capable of deciphering the major processes in microbial communities, but the complete analysis of their interactions with their host is still in its infancy. In order to get easier access, the study of nonmammalian model organisms bears great potential. These organisms provide advantages such as reduced complexity, ease of cultivation in great numbers, and amenity to a range of genetic and biochemical manipulations. We highlight the potentials provided by model organism proteomics for the study of host–microbiome interactions and outline major challenges and demands for technological improvements that will be necessary for the understanding of the manifold interactions within meta-organisms.     

A conserved deamidation site at Asn 2 in the catalytic subunit of mammalian cAMP-dependent protein kinase detected by capillary LC-MS and tandem mass spectrometry

The N-terminal sequence myr-Gly-Asn is conserved among the myristoylated cAPK (protein kinase A) catalytic subunit isozymes Calpha, Cbeta, and Cgamma. By capillary LC-MS and tandem MS, we show that, in approximately one third of the Calpha and Cbeta enzyme populations from cattle, pig, rabbit, and rat striated muscle, Asn 2 is deamidated to Asp 2. This deamidation accounts for the major isoelectric variants of the cAPK C-subunits formerly called CA and CB. Deamidation also includes characteristic isoaspartate isomeric peptides from Calpha and Cbeta. Asn 2 deamidation does not occur during C-subunit preparation and is absent in recombinant myristoylated Calpha (rCalpha) from Escherichia coli. Deamidation appears to be the exclusive pathway for introduction of an acidic residue adjacent to the myristoylated N-terminal glycine, verified by the myristoylation negative phenotype of an rCalpha(Asn 2 Asp) mutant. This is the first report thus far of a naturally occurring myr-Gly-Asp sequence. Asp 2 seems to be required for the well-characterized (auto)phosphorylation of the native enzyme at Ser 10. Our results suggest that the myristoylated N terminus of cAPK is a conserved site for deamidation in vivo. Comparable myr-Gly-Asn sequences are found in several signaling proteins. This may be especially significant in view of the recent knowledge that negative charges close to myristic acid in some proteins contribute to regulating their cellular localization.     

Demonstration of WDM survivable unidirectional ring network usingtunable channel dropping receivers

An experimental optical WDM reconfigurable ring network is proposed. It is composed of three nodes incorporating tunable channel dropping receivers (TCDRs) and EDFAs. The reconfiguration and the protection of the ring are computer-controlled. The authors present first experimental results of a fully connected testbed demonstrator     

First low polarisation dependence optical FSK receiver based on a Fabry-Perot laser with a near square active waveguide structure

A low polarisation dependent optical FSK receiver based on an FP laser with one antireflection coated facet and a near square active waveguide structure is reported. The FP laser receiver presents a polarisation-dependence of less than 2 dB.<>     

Approximation Algorithms for Intersection Graphs

We study three complexity parameters that, for each vertex v, are an upper bound for the number of cliques that are sufficient to cover a subset S(v) of its neighbors. We call a graph k-perfectly groupable if S(v) consists of all neighbors, k-simplicial if S(v) consists of the neighbors with a higher number after assigning distinct numbers to all vertices, and k-perfectly orientable if S(v) consists of the endpoints of all outgoing edges from v for an orientation of all edges. These parameters measure in some sense how chordal-like a graph is—the last parameter was not previously considered in literature. The similarity to chordal graphs is used to construct simple polynomial-time approximation algorithms with constant approximation ratio for many NP-hard problems, when restricted to graphs for which at least one of the three complexity parameters is bounded by a constant. As applications we present approximation algorithms with constant approximation ratio for maximum weighted independent set, minimum (independent) dominating set, minimum vertex coloring, maximum weighted clique, and minimum clique partition for large classes of intersection graphs.