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1.
Two screening methods for urine microbiology are proposed: one in which the Gram-stained smear is used to detect significant bacteriuria, and another in which Autobac antibiotic susceptibility tests are performed directly on positive urine samples. Results on 1,350 specimens indicated that an average of 18 bacteria per oil immersion field were observed in the urine of patients with significant bacteriuria, and an average of less than 1 bacterium per oil immersion field was found in the urine of patients without significant bacteriuria. Direct susceptibility testing by Autobac proved to be rapid (3 h versus 24 h) and reliable (0.5 to 1.2% discrepancies). 相似文献
2.
The effects of gamma radiation doses between 5 and 100 krad on all metamorphic stages of Tribolium madens and T. castaneum were studied. Results from the two species were similar although T. madens appeared to be more radiosensitive. The development of adults from treated eggs and larvae was prevented by 5 krad in T. madens and by 10 krad in T. castaneum. Some adults emerged from treated pupae at all treatment levels, but no reproduction occurred at 20 krad or above. Adult females of both species were more sensitive to the sterilizing effects of gamma radiation than were males, but sterility of both sexes was effected at doses of 30 krad and above. Control of these two species appears feasible with levels of radiation currently approved for treatment of stored-grain insects in wheat and wheat flour. 相似文献
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Tilton M.L. Dente G.C. Paxton A.H. Cser J. DeFreez R.K. Moeller C.E. Depatie D. 《Quantum Electronics, IEEE Journal of》1991,27(9):2098-2108
The authors describe an on-the-chip design of a nearly diffraction-limited broad-area semiconductor diode laser. The device achieved single lateral mode operation as unstable resonators with magnifications between two and three. The unstable resonators were realized by focused ion beam (FIB) micromachining a diverging mirror at one of the outcoupling facets. The modeling efforts agree well with experimental data and show that an optimum device design exists in which stable nearly diffraction-limited operation is predicted for up to six times threshold. This unstable resonator design has achieved, experimentally, the highest diffraction limited power and best external differential efficiency ever reported for any broad-area device with a curved facet 相似文献
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Henry C. W. Chu Stephen Garoff Robert D. Tilton Aditya S. Khair 《American Institute of Chemical Engineers》2020,66(2):e16831
A multiple-scale perturbation is conducted to derive an averaged equation for predicting the longtime solute transport in an eccentric annulus in which the uniaxial flow may oscillate periodically in time. A proof for the positiveness of the dispersivity is presented, implying that over a cycle of oscillation a solute cloud always broadens. For a steady flow driven by a fixed pressure gradient, increasing the eccentricity and annulus size gives rise to stronger dispersion. This relationship holds when the flow becomes unsteady. In the limit of slow oscillation, dispersion due to an oscillatory flow asymptotes to one-half of that by a steady flow. Increasing the oscillation frequency leads to a two-step decay of the dispersivity. The maximum dispersion in an oscillatory flow can be achieved in the limit of slow oscillation and large eccentricity, where dispersion can be O(103) times larger than that in an otherwise concentric annulus. 相似文献
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Titanium dioxide (P25) produces reactive oxygen species in immortalized brain microglia (BV2): implications for nanoparticle neurotoxicity 总被引:15,自引:0,他引:15
Long TC Saleh N Tilton RD Lowry GV Veronesi B 《Environmental science & technology》2006,40(14):4346-4352
Concerns with the environmental and health risk of widely distributed, commonly used nanoparticles are increasing. Nanosize titanium dioxide (TiO2) is used in air and water remediation and in numerous products designed for direct human use and consumption. Its effectiveness in deactivating pollutants and killing microorganisms relates to photoactivation and the resulting free radical activity. This property, coupled with its multiple potential exposure routes, indicates that nanosize TiO2 could pose a risk to biological targets that are sensitive to oxidative stress damage (e.g., brain). In this study, brain microglia (BV2) were exposed to a physicochemically characterized (i.e., dispersion stability, particle size distribution, and zeta potential) nanomaterial, Degussa P25, and cellular expressions of reactive oxygen species were measured with fluorescent probes. P25's zeta potentials, measured in cell culture media and physiological buffer were -11.6 +/- 1.2 mV and -9.25 +/- 0.73 mV, respectively. P25 aggregation was rapid in both media and buffer with the hydrodynamic diameter of stable P25 aggregates ranging from 826 nm to 2368 nm depending on the concentration. The biological response of BV2 microglia to noncytotoxic (2.5-120 ppm) concentrations of P25 was a rapid (<5 min) and sustained (120 min) release of reactive oxygen species. The time course of this release suggested that P25 not only stimulated the immediate "oxidative burst" response in microglia but also interfered with mitochondrial energy production. Transmission electron microscopy indicated that small groups of nanosized particles and micron-sized aggregates were engulfed bythe microglia and sequestered as intracytoplasmic aggregates after 6 and 18 h exposure to P25 (2.5 ppm). Cell viability was maintained at all test concentrations (2.5-120 ppm) over the 18 h exposure period. These data indicate that mouse microglia respond to Degussa P25 with cellular and morphological expressions of free radical formation. 相似文献
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Segmented mirror phasing using the focal-plane intensity 总被引:1,自引:0,他引:1
We develop a method for subaperture piston phase retrieval in a telescope using a segmented primary mirror. We assume that the mirror subapertures are arranged on a two-dimensional lattice, and in addition, the separate subaperture point-spread functions are focused and overlapped on the focal plane. Therefore, the residual errors are the subaperture piston phase errors, represented as a phasor, a unit modulus complex number, for each subaperture. Under these conditions, we find considerable simplicity in the calculated optical transfer function (OTF) at special subaperture lattice spatial frequencies. We then construct a phasor-based error function based on the modulus squared of the difference between the measured OTF and the calculated OTF. The remaining steps in our piston phase retrieval algorithms are developed by calculating the error-function variation, with respect to each phasor element. The resulting equations for the error gradient are then used iteratively, in a phasor-based algorithm, to find the minimum of the error function. In the applications, we simulate photon-noise-limited piston retrieval for a segmented primary with 18 hexagonal subapertures. When we invoke phase diversity, the piston retrievals prove unique and accurate. 相似文献
10.
Nils Tilton Denis Martinand Eric Serre Richard M. Lueptow 《American Institute of Chemical Engineers》2012,58(7):2030-2044
A generalized solution for pressure‐driven, incompressible, Newtonian flow in a porous tubular membrane is challenging due to the coupling between the transmembrane pressure and velocity. To date, all analytical solutions require simplifications such as neglecting the coupling between the transmembrane pressure and velocity, assuming the form of the velocity fields, or expanding in powers of parameters involving the tube length. Moreover, previous solutions have not been validated with comparison to direct numerical simulation (DNS). We comprehensively revisit the problem to present a robust analytical solution incorporating Darcy's law on the membrane. We make no assumptions about the tube length or form of the velocity fields. The analytic solution is validated with detailed comparison to DNSs, including cases of axial flow exhaustion and cross flow reversal. We explore the validity of typical assumptions used in modeling porous tube flow and present a solution for porous channels in Supporting Information. © 2012 American Institute of Chemical Engineers AIChE J, 2012 相似文献