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Pal Tumpa Saha Ramesh Biswas Suparna 《International Journal of Wireless Information Networks》2022,29(3):373-392
International Journal of Wireless Information Networks - In this work, energy efficient routing protocol variants considering different sink mobility in hierarchical cluster based wireless sensor... 相似文献
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Tumpa Dutta Amit Kumar Das Debabrata Das 《International Journal of Hydrogen Energy》2009,34(17):7530-7537
Fe-hydrogenase from Enterobacter cloacae IIT-BT08 was purified 1284 fold with specific activity of 335 μmol H2/min/mg protein for hydrogen evolution using reduced methyl viologen as an electron-donor at 25 °C. The molecular weight of the monomeric enzyme was determined to be 51 kDa by MALDI-ToF mass spectrometry. The PI of the enzyme was 5.6 displaying its acidic nature. The optimal temperature and pH for hydrogen evolution was 37 °C and 7–7.2 respectively. The affinity constant, Km for reduced methyl viologen was 0.57 ± 0.03 mM and that of reduced ferredoxin was 0.72 ± 0.04 μM. The enzyme contained 11.47 gm-atom Fe/mol of Fe-hydrogenase. Electron paramagnetic resonance analysis ascertained the existence of iron molecules as [4Fe–4S] clusters. The internal amino acid sequences of trypsin digested peptides of hydrogenase as determined by ESI MS/MS Q-ToF showed 80-87% identities with the respective sequences of Clostridium sp. and Trichomonas sp. hydrogenase. 相似文献
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