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S Turki L Guillevin A Dallot B Jarrousse I Vernier L Laroche J Pourrat J Amouroux 《Canadian Metallurgical Quarterly》1994,121(4):325-327
Human herpesvirus type 6 DNA derived from human breath was discovered to contaminate PCR reactions during routine reaction preparation. Parallel PCR experiments were conducted in which expiratory secretions were blocked by a surgical mask, while others were performed without any attempt to circumvent respiratory contamination. The experimenter was previously determined to harbor HHV-6 DNA in the saliva. All reactions in which expiration was obstructed were negative for HHV-6 DNA via PCR. Reactions in which there was no attempt to obstruct respiratory secretions were positive for HHV-6 DNA. These data suggest that PCR assays investigating the presence of HHV-6 may be highly susceptible to contamination from the experimenter leading to false positive results. 相似文献
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A country’s economy heavily depends on agricultural development. However, due to several plant diseases, crop growth rate and quality are highly suffered. Accurate identification of these diseases via a manual procedure is very challenging and time-consuming because of the deficiency of domain experts and low-contrast information. Therefore, the agricultural management system is searching for an automatic early disease detection technique. To this end, an efficient and lightweight Deep Learning (DL)-based framework (E-GreenNet) is proposed to overcome these problems and precisely classify the various diseases. In the end-to-end architecture, a MobileNetV3Small model is utilized as a backbone that generates refined, discriminative, and prominent features. Moreover, the proposed model is trained over the PlantVillage (PV), Data Repository of Leaf Images (DRLI), and a new Plant Composite (PC) dataset individually, and later on test samples, its actual performance is evaluated. After extensive experimental analysis, the proposed model obtained 1.00%, 0.96% and 0.99% accuracies on all three included datasets. Moreover, the proposed method achieves better inference speed when compared with other State-Of-The-Art (SOTA) approaches. In addition, a comparative analysis is conducted where the proposed strategy shows tremendous discriminative scores as compared to the various pre-trained models and other Machine Learning (ML) and DL methods. 相似文献
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Yousra Turki Hada Ouzari Ines Mehri Aouatef Ben Ammar Abdennaceur Hassen 《Food research international (Ottawa, Ont.)》2012,45(2):1099-1105
Salmonella serovars are increasing in importance as significant pathogens of both human and animals. Although water and wastewater are treated to eliminate pathogenic microorganisms, they still play an important role in the transmission of Salmonella spp. In this study, bacteriophages infecting Salmonella spp. were isolated from wastewater and evaluated; for their potential to lyse environmental Salmonella strains in vitro at different MOIs and temperatures; and to control the wastewater bacterial community. Three distinct phages designated sww65, sww275, and sww297; as defined by plaque morphology, electron microscopy and host range; were obtained from wastewater. Challenge tests were performed at 37, and 30 °C with the infection of the Salmonella cultures with individual phage, a mixture of two phages, and cocktail of three phages at MOIs of 100, 102, and 104 PFU/CFU. At 30, and 37 °C, a cocktail of three phages reduced all of the Salmonella cultures tested. These results required a high multiplicity of infection. However, when infected with only one phage or a mixture of two phages at MOIs of 100 or 10 2 PFU/CFU, an emergence of bacterial resistance was observed. The dynamic monitoring of wastewater enterobacterial community was conducted using Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR). The number of bands decreased gradually with the use of individual phage or phage cocktails. Moreover, the dynamic monitoring of Salmonella community during wastewater treatment was performed using PCR detection of virulence gene invA. The results correlated with the ERIC-PCR fingerprints, and suggested that Salmonella community was affected by the phage treatment. Indeed, in wastewater, bacteriophages are reducing Salmonella and other members of the Enterobacteriaceae. These results indicated that dynamic changes are closely related with the process of treatment. The introduction of wide host range bacteriophages in wastewater can have a potential impact on the dynamics of the microbial communities, manifested by the reduction or the elimination of microbial species. 相似文献
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Houcemeddine Turki 《Scientometrics》2016,106(3):1281-1282
7.
M. Turki E. Bretagne M.J. Rouis M. Quéneudec 《Construction and Building Materials》2009,23(7):2715-2722
This work focuses on the feasibility of reusing and valorising rubber aggregates from shredded worn tyres in cement composites. Our purpose is to study the influence of both microstructures of matrix and of interfacial transition zone (ITZ) on mechanical properties of mortar–rubber aggregates mixtures. The same volume of rubber aggregates substituted volume fractions of sand. Volume ratios of rubber aggregates were ranged from 0% to 50%. Micro structural morphology of various samples was observed by means of scanning electron microscopy (SEM). The matrix porosity and the evolution of hydration products (CSH, CaOH2, CaCO3) were estimated for specimens that had been curing for 28 days at constant temperature and constant relative humidity. An energy dispersive X-ray analyser and X-ray diffraction were used together with SEM in order to quantify these results. 相似文献
8.
Mejda Ajili Najoua Turki Kamoun 《Journal of Materials Science: Materials in Electronics》2014,25(9):3840-3845
Indium sulfide (In2S3) is a good window or buffer layer for photovoltaic application. In this work, β-In2?xAlxS3 thin films with different thicknesses (400, 442, 646 and 714 nm) are successfully synthesized on heated glass substrates using a chemical spray pyrolysis technique. The thin film thickness effect on the structural, optical and photoluminescence (PL) properties of β-In2?xAlxS3 material is studied. The X-ray diffraction patterns suggest the formation of β-In2S3 cubic phase preferentially oriented towards (400) direction. The level of the residual dislocation seems to be reduced to 3.12 × 109 lines mm?2 for the optimum thickness (646 nm) for which the β-In2?xAlxS3 film crystallinity is the best one. In order to enhance the electrical properties, β-In2?xAlxS3 layers are annealed in air at 400 °C for different annealing times (15, 30 and 45 min). The minimum resistivity, maximum Hall mobility and carrier concentration are found for β-In2?xAlxS3 films annealed for 30 min. All samples have high transmittance of about 75 % but the wide band gap (Eg = 3.32 eV) is obtained for this optimum thickness. This result indicates good optical quality of β-In2?xAlxS3 layers. Defects-related PL properties are also discussed. 相似文献
9.
Microsystems technology is an emerging technology that is used in ever more technical systems, such as inkjet nozzles or accelerometers. 3D micromechanical structures became feasible by miniaturizing macromechanical elements. One big challenge in downsizing mechanical elements is to adapt conventional manufacturing technologies to the conditions of microtechnology. Integrated design and production processes must be suited to the new requirements, in-depth knowledge of production technology is required to design micromechanical components. Since 2000, the Collaborative Research Center 499 “Design, Production and Quality Assurance of Molded Microcomponents made of Metallic and Ceramic Materials” has been focusing on a continuous and stable process chain for molded micro components (Albers et al. 2005, Microsyst Technol). The aim of prior funding periods was to develop the knowledge about how components, such as gearwheels, and systems, such as micro gears, can be designed. Present research is focused on how to design functional elements, i.e. shaft-hub joints to transmit torque. This paper will introduce an approach that generates know-how via testing several plain bearings and shaft-hub joints in order to derive the necessary know-how to develop simulation tools later on. 相似文献
10.
Khan Muhammad Yasar Zuhairi Megat F. Ali Toqeer Alghamdi Turki Marmolejo-Saucedo Jose Antonio 《Wireless Networks》2020,26(7):4943-4954
Wireless Networks - In distributed environment, a digital transaction or operation requires transparency and trust among multiple stakeholders. Several approches address such... 相似文献