High hydrostatic pressure: a new way to improve in vitro developmental competence of porcine matured oocytes after vitrification

The purpose of the present study was to improve cryotolerance using high hydrostatic pressure (HHP) pretreatment of porcine in vitro matured (IVM) oocytes, to facilitate their further developmental competence after parthenogenetic activation. A total of 1668 porcine IVM oocytes were used in our present study. The pressure tolerance and optimal duration of recovery after HHP treatment were determined. Oocytes were treated with either 20 or 40 MPa (200 and 400 times greater than atmospheric pressure) for 60 min, with an interval of 10, 70, and 130 min between pressure treatment and subsequent vitrification under each pressure parameter. Oocytes from all vitrification groups had much lower developmental competence than fresh oocytes (P<0.01) measured as cleavage and blastocyst rates. However, significantly higher blastocyst rates (P<0.01) were obtained in the groups of 20 MPa pressure, with either 70 (11.4+/-2.4%) or 130 (13.1+/-3.2%) min recovery, when compared with the vitrification control group without HHP treatment where no blastocysts were obtained. The influence of temperature at HHP treatment on further embryo development was also investigated. Treatments of 20 MPa with 70 min recovery were performed at 37 degrees C or 25 degrees C. Oocytes pressurized at 37 degrees C had a significantly higher blastocyst (14.1+/-1.4%) rate than those treated at 25 degrees C (5.3+/-1.1%; P<0.01). Our results demonstrate that HHP pretreatment could considerably improve the developmental competence of vitrified pig in vitro matured (IVM) oocytes. The HHP pretreatment will be tested as a means to improve survival and developmental competence at different developmental stages in different species including humans.     

A Backward-Compatible Multichannel Audio Codec

We propose in this paper a backward-compatible multichannel audio codec. This codec represents a multichannel audio input signal by a down mix and parametric data. In order to enable backward compatibility, it is necessary to have the possibility of exerting control over the down-mixing procedure. At the same time, in order to achieve a high coding efficiency, both signal and perceptual redundancies should be exploited. In this paper, we describe a codec that unifies the above-mentioned conditions: backward compatibility and exploitation of both signal and perceptual redundancies. The codec combines a high audio quality and a low parameter bit rate. Moreover, its design is flexible, examples of which are the scalability of the audio quality to (in principle) transparency and the possibility to preserve the correlation structure of the original input signals by using synthetic signals. A stereo backward compatible version of the proposed codec is used as a component of the recently standardized MPEG Surround multichannel audio codec.     

Controlled trial of penfluridol and thiothixene in the maintenance treatment of chronic schizophrenic syndromes

In a controlled trial of penfluridol and thiothixene as maintenance drugs in patients with chronic schizophrenic syndromes, some improvement over previous neuroleptics was seen with both drugs. This improvement was mainly evident in variables concerned with participation in social activities as assessed with the S-scale and by ward behaviour. The drug dosages necessary were very low and gave few and easily manageable side-effects. There was no significant difference between penfluridol and thiothixene. Penfluridol has the clear practical advantage of being the only long-acting drug for oral administration so far available.     

Comparison of noise sources in dual- and single-beam Fourier-transform near-infrared spectrometry

We report the results of noise source investigations and stability tests in both dual- and single-beam Fourier-transform near-infrared operation. The noise sources are divided into two parts: intrinsic and extrinsic. The intrinsic noise sources, which include detector system noise, are common for both modes of operation. The extrinsic sources, which include variations in ambient conditions (room temperature, atmospheric gaseous components, and source scintillations), are shown to be smaller in dual-beam operation than in single-beam operation by a factor of 2-10. The results are based on interferograms measured in specified time intervals. The root-mean-square values are calculated at each retardation point. The values obtained near the centerburst and average values obtained for the dual-beam operation are compared with the intrinsic noise value obtained for single-beam operation. The dual-beam advantage is observed in both open-beam and liquid cell measurements, and it corresponds well with earlier results based on multivariate calibration techniques applied on aqueous solutions.     

Reduction of correlated noise using a library of orthonormal bases

We study the application of a library of orthonormal bases to the reduction of correlated Gaussian noise. A joint condition on the library and the noise covariance is derived which ensures that simple thresholding in an adaptively chosen basis yields an estimation error within a logarithmic factor of the ideal risk. In the model example of a wavelet packet library and stationary noise the condition can be translated into a reverse Holder inequality on the power spectrum