Antitumor gene therapy using suicide genes]

Successful autologous bone marrow transplantation (ABMT) and peripheral blood stem cell transplantation depend on safe hematopoietic stem cell (HSC) cryopreservation and storage. Several successful methods for cryopreservation and storage have been established and are commonly used all over the world. However, little is known about the effects of long-term cryopreservation on the capacity to sustain a complete immunohematopoietic engraftment. Several authors have investigated stem cell viability after cryopreservation and storage for more than 5 years and reported preclinical good viabilities in terms of dye-exclusion or colony-forming capability in vitro. Only two studies using BM cryopreserved for more than 5 years for transplantation are reported, but they did not provide proof of trilineage engraftment. In February 1997 at our institution, a patient with relapsed non-Hodgkin's lymphoma underwent ABMT with BM harvested in February 1990. He achieved a granulocyte count > 500 x 10(6)/L on day 21 and a self-supporting platelet count > 20 x 10(9)/L on day 30. After day 29, his hemoglobin level was satisfactory without need of transfusion support. This successful trilineage engraftment with cryopreserved BM that had been stored for 7 years suggests that HSC are able to maintain their capability to reconstitute hematopoiesis for a long time.     

Binary images of cyclic codes over Z4

We determine all linear cyclic codes over Z₄ of odd length whose Gray images are linear codes (or, equivalently, whose Nechaev-Gray (1989) image are linear cyclic codes or are linear cyclic codes)     

The weights of the orthogonals of the extended quadratic binaryGoppa codes

Starting from results on elliptic curves and Kloosterman sums over the finite field GE(2^t), the authors determine the weights of the orthogonals of some binary linear codes; the Melas code of length, the irreducible cyclic binary code of length 2^t+1, and the extended binary Goppa codes defined by polynomials of degree two