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1.
Goren Y Davrath LR Pinhas I Toledo E Akselrod S 《IEEE transactions on bio-medical engineering》2006,53(1):35-42
Heart rate variability (HRV) is a major noninvasive technique for evaluating the autonomic nervous system (ANS). Use of time-frequency approach to analyze HRV allows investigating the ANS behavior from the power integrals, as a function of time, in both steady-state and non steady-state. Power integrals are examined mainly in the low-frequency and the high-frequency bands. Traditionally, constant boundaries are chosen to determine the frequency bands of interest. However, these ranges are individual, and can be strongly affected by physiologic conditions (body position, breathing frequency). In order to determine the dynamic boundaries of the frequency bands more accurately, especially during autonomic challenges, we developed an algorithm for the detection of individual time-dependent spectral boundaries (ITSB). The ITSB was tested on recordings from a series of standard autonomic maneuvers with rest periods between them, and the response to stand was compared to the known physiological response. A major advantage of the ITSB is the ability to reliably define the mid-frequency range, which provides the potential to investigate the physiologic importance of this range. 相似文献
2.
Oleogels from Glycerol‐Based Lyotropic Liquid Crystals: Phase Diagrams and Structural Characterization 下载免费PDF全文
Yael Cegla‐Nemirovsky Abraham Aserin Nissim Garti 《Journal of the American Oil Chemists' Society》2015,92(3):439-447
In the course of our studies on structured reverse lyotropic liquid crystals (LLC) as oleogels, a system was designed with the desired physical and rheology properties for cosmetic and pharmaceutical applications. The aqueous phase was partially replaced by glycerol to minimize bacteriological problems and obtain specific rheology characteristics. The constructed phase diagrams are based on ternary mixtures of glycerol monooleate (GMO), decane, water, and glycerol. The main lyotropic mesophase obtained in this study was reverse hexagonal derived from dilution line 8:2 (72 wt% GMO and 18 wt% decane) and 10 wt% water; or water:glycerol (wt ratios 3:1 and 1:1) mixture. It was found that heat‐cool fluctuation caused formation of new pseudo‐equilibrium structures with mesomorphic transformations to more fluid and less ordered mesostructures. Replacing up to 50 wt% of the water by glycerol significantly increases the gel phase region in the phase diagram, meaning more structural compositional options. The structural parameters were determined using cross‐polarized light microscopy (CPLM), differential scanning calorimeter (DSC), powder X‐ray diffraction (PXRD), and small angle X‐ray scattering (SAXS). Rheological measurements revealed viscoelastic properties of lyotropic liquid crystals with a decrease in the elasticity (G′), plasticity (G″), and complex viscosity (η*), as a function of increasing the glycerol content. 相似文献
3.
Sigal Avraham Dr. Leonie Schütz Dr. Larissa Käver Dr. Andreas Dankers Sapir Margalit Dr. Yael Michaeli Dr. Shahar Zirkin Dr. Dmitry Torchinsky Dr. Noa Gilat Omer Bahr Gil Nifker Prof. Maya Koren-Michowitz Prof. Elmar Weinhold Prof. Yuval Ebenstein 《Chembiochem : a European journal of chemical biology》2023,24(20):e202300400
5-Methylcytosine and 5-hydroxymethylcytosine are epigenetic modifications involved in gene regulation and cancer. We present a new, simple, and high-throughput platform for multi-color epigenetic analysis. The novelty of our approach is the ability to multiplex methylation and de-methylation signals in the same assay. We utilize an engineered methyltransferase enzyme that recognizes and labels all unmodified CpG sites with a fluorescent cofactor. In combination with the already established labeling of the de-methylation mark 5-hydroxymethylcytosine via enzymatic glycosylation, we obtained a robust platform for simultaneous epigenetic analysis of these marks. We assessed the global epigenetic levels in multiple samples of colorectal cancer and observed a 3.5-fold reduction in 5hmC levels but no change in DNA methylation levels between sick and healthy individuals. We also measured epigenetic modifications in chronic lymphocytic leukemia and observed a decrease in both modification levels (5-hydroxymethylcytosine: whole blood 30 %; peripheral blood mononuclear cells (PBMCs) 40 %. 5-methylcytosine: whole blood 53 %; PBMCs 48 %). Our findings propose using a simple blood test as a viable method for analysis, simplifying sample handling in diagnostics. Importantly, our results highlight the assay‘s potential for epigenetic evaluation of clinical samples, benefiting research and patient management. 相似文献
4.
A brief proton pulse technique, based on photo-excitation of pyranine, was employed for measuring the protonation dynamics of cytochrome c in aqueous solutions. Time-resolved monitoring of the protonation state of the pyranine anion yielded detailed information from which the temporal state protonation of the surface carboxylates and histidine residues were deduced. The surface groups were found to be coupled by electrostatic interaction where the state of protonation of one affects the pK of the others. In the same sense, altering the charge distribution of a protein by a redox reaction affects the reactivity of the surface groups with protons in the bulk. The surface groups can exchange protons among themselves at a very high rate. The velocities of these reactions are functions of the connectivity between the proton binding sites. A single proton exchange reaction between the surface groups could be identified as the proton exchange between H26 and E44. The reaction is fast and affected by the redox state of the protein. It is proposed that the enhanced rate of this reaction is coupled with transient conformational changes that are not noticeable in time-averaging measurements such as X-ray diffraction or NMR. 相似文献
5.
A novel controlled release formulation (CRF) of the herbicide imazapyr (IMP) was designed to reduce its leaching,which causes soil and water contamination. The anionic herbicide IMP was bound to polydiallyldimethylammonium-chloride (PDADMAC)-montmorillonite composites. PDADMAC adsorption reached a high loading of polymer, which resulted in charge reversal of the clay and promoted IMP binding. The composites were characterized by Fourier transform infrared, zeta potential, and X-ray diffraction measurements, indicating electrostatic interactions of the polycation with the surface, polycation intercalation in the clay and suggesting a configuration as loops and tails on the surface at high loadings. IMP binding to the composites is affected by polycation loading and flocculation. Upon adding high concentrations of IMP to a composite of 0.16 g/g, we obtained high herbicide loadings (66% active ingredient). IMP release from the CRFs applied on a thin layer of soil was substantially slower than its release from the commercial formulation (Arsenal). Accordingly, soil column bioassays indicated reduced herbicide leaching (nearly 2-fold) upon applying the CRFs while maintaining good herbicidal activity. The new PDADMAC-clay formulations are promising from the environmental and weed control management points of view. 相似文献
6.
Novel functional foods, such as tomato juice with soy, represent a new strategy to increase consumption of health promoting ingredients and phytochemicals. Interactions between soy protein, isoflavones, and tomato carotenoids could impact the bioaccessibility and bioavailability of individual phytochemicals. The primary objective of this study was to assess possible interactions between daidzein and daidzin, soy protein and carotenoids using proton one-dimensional and two-dimensional pulsed field gradient nuclear magnetic resonance spectroscopy experiments. 相似文献
7.
Polarized unification grammar (PUG) is a linguistic formalism which uses polarities to better control the way grammar fragments interact. The grammar combination operation of PUG was conjectured to be associative.
We show that PUG grammar combination is not associative, and even attaching polarities to objects does not make it order-independent. Moreover, we prove that no non-trivial polarity system exists for which grammar combination is associative. We then redefine the grammar combination operator, moving to the powerset domain, in a way that guarantees associativity.
The method we propose is general and is applicable to a variety of tree-based grammar formalisms. 相似文献
8.
Binding of pyrene, fluoranthene, and phenanthrene to dissolved humic acids (HA) was determined by the fluorescence quenching (FQ) and complexation-flocculation (CF) methods. Determinations by the CF method, using varying contaminant concentrations and a constant HA concentration, yielded nonlinear Freundlich-type isotherms (n = 0.65-0.84). Experiments using both the CF and the FQ methods with varying HA concentrations and a constant contaminant concentration yielded curved "Stern-Volmer"-type plots that also indicate nonlinear binding. These findings suggest that linear partitioning or site complexation in the presence of excess available sites cannot fully describe the interactions of hydrophobic compounds with dissolved humic material. Site-specific hydrophobic interactions at limited interior or external molecular surfaces may be considered. 相似文献
9.
One approach to recognizing objects seen from arbitrary viewpoint is by extracting invariant properties of the objects from single images. Such properties are found in images of 3D objects only when the objects are constrained to belong to certain classes (e.g., bilaterally symmetric objects). Existing studies that follow this approach propose how to compute invariant representations for a handful of classes of objects. A fundamental question regarding the invariance approach is whether it can be applied to a wide range of classes. To answer this question it is essential to study the set of classes for which invariance exists. This paper introduces a new method for determining the existence of invariant functions for classes of objects together with the set of images from which these invariants can be computed. We develop algebraic tests that determine whether the objects in a given class can be identified from single images. These tests apply to classes of objects undergoing affine projection. In addition, these tests allow us to determine the set of views of the objects which are degenerate. We apply these tests to several classes of objects and determine which of them is identifiable and which of their views are degenerate. 相似文献
10.
Machine Learning - In survival analysis, estimating the failure time distribution is an important and difficult task, since usually the data is subject to censoring. Specifically, in this paper we... 相似文献