CaDHN3, a Pepper (Capsicum annuum L.) Dehydrin Gene Enhances the Tolerance against Salt and Drought Stresses by Reducing ROS Accumulation

Dehydrins (DHNs) play an important role in abiotic stress tolerance in a large number of plants, but very little is known about the function of DHNs in pepper plants. Here, we isolated a Y₁SK₂-type DHN gene “CaDHN3” from pepper. To authenticate the function of CaDHN3 in salt and drought stresses, it was overexpressed in Arabidopsis and silenced in pepper through virus-induced gene silencing (VIGS). Sub-cellular localization showed that CaDHN3 was located in the nucleus and cell membrane. It was found that CaDHN3-overexpressed (OE) in Arabidopsis plants showed salt and drought tolerance phenotypic characteristics, i.e., increased the initial rooting length and germination rate, enhanced chlorophyll content, lowered the relative electrolyte leakage (REL) and malondialdehyde (MDA) content than the wild-type (WT) plants. Moreover, a substantial increase in the activities of antioxidant enzymes; including the superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and lower hydrogen peroxide (H₂O₂) contents and higher O₂^•− contents in the transgenic Arabidopsis plants. Silencing of CaDHN3 in pepper decreased the salt- and drought-stress tolerance, through a higher REL and MDA content, and there was more accumulation of reactive oxygen species (ROS) in the CaDHN3-silenced pepper plants than the control plants. Based on the yeast two-hybrid (Y2H) screening and Bimolecular Fluorescence Complementation (BiFC) results, we found that CaDHN3 interacts with CaHIRD11 protein in the plasma membrane. Correspondingly, the expressions of four osmotic-related genes were significantly up-regulated in the CaDHN3-overexpressed lines. In brief, our results manifested that CaDHN3 may play an important role in regulating the relative osmotic stress responses in plants through the ROS signaling pathway. The results of this study will provide a basis for further analyses of the function of DHN genes in pepper.     

The availability of daylight from tropical skies—a case study of Malaysia

In Malaysia, no long-term daylight data are measured. It was only recently that the need to measure the availability of daylight became urgent when the importance of daylighting in buildings was rediscovered. The hourly daylight availability has been simulated for the Malaysian sky using daylight modelling techniques based on empirical and measured solar irradiation and cloud cover data. This paper presents the techniques involved in producing exterior illuminance data. These data were then compared with measured illuminance at Shah Alam and Bangi, Malaysia. The global illuminance levels are generally high, with values exceeding 80,000 lux at noon during the months when solar irradiation is highest. Even during the months when the ground receives less solar irradiation, the peak illuminance can reach 60,000 lux. Applications and uses of such data are in daylighting design, both for visual and thermal comfort, task illuminance and energy-conscious design of buildings. Recommendations are made at the end of the paper on the various climatic data that are required to be measured for overall daylighting design applications.     

Exploring album structure for face recognition in online social networks

In this paper, we propose an album-oriented face-recognition model that exploits the album structure for face recognition in online social networks. Albums, usually associated with pictures of a small group of people at a certain event or occasion, provide vital information that can be used to effectively reduce the possible list of candidate labels. We show how this intuition can be formalized into a model that expresses a prior on how albums tend to have many pictures of a small number of people. We also show how it can be extended to include other information available in a social network. Using two real-world datasets independently drawn from Facebook, we show that this model is broadly applicable and can significantly improve recognition rates.     

Scientific Workflow Makespan Minimization in Edge Multiple Service Providers Environment

The edge computing model offers an ultimate platform to support scientific and real-time workflow-based applications over the edge of the network. However, scientific workflow scheduling and execution still facing challenges such as response time management and latency time. This leads to deal with the acquisition delay of servers, deployed at the edge of a network and reduces the overall completion time of workflow. Previous studies show that existing scheduling methods consider the static performance of the server and ignore the impact of resource acquisition delay when scheduling workflow tasks. Our proposed method presented a meta-heuristic algorithm to schedule the scientific workflow and minimize the overall completion time by properly managing the acquisition and transmission delays. We carry out extensive experiments and evaluations based on commercial clouds and various scientific workflow templates. The proposed method has approximately 7.7% better performance than the baseline algorithms, particularly in overall deadline constraint that gives a success rate.

     

Eco‐friendly synthesis of silver nanoparticles and its larvicidal property against fourth instar larvae of Aedes aegypti

In this study, larvicidal activity of silver nanoparticles (AgNPs) synthesised using apple extract against fourth instar larvae of Aedes aegypti was determined. As a result, the AgNPs showed moderate larvicidal effects against Ae. aegypti larvae (LC₅₀  = 15.76 ppm and LC₉₀  = 27.7 ppm). In addition, comparison of larvicidal activity performance of AgNPs at high concentration prepared using two different methods showed that Ae. aegypti larvae was fully eliminated within the duration of 2.5 h. From X‐ray diffraction, the AgNP crystallites were found to exhibit face centred cubic structure. The average size of these AgNPs as estimated by particle size distribution was in the range of 50–120 nm. The absorption maxima of the synthesised Ag showed characteristic Ag surface plasmon resonance peak. This green synthesis provides an economic, eco‐friendly and clean synthesis route to Ag.Inspec keywords: silver, nanofabrication, X‐ray diffraction, zoology, particle size, nanoparticles, biomedical materials, nanomedicineOther keywords: time 2.5 h, size 50 nm to 120 nm, silver nanoparticle, larvicidal property, instar larvae, Aedes aegypti, larvicidal effect, larvicidal activity performance, X‐ray diffraction, nanoparticle particle size distribution, absorption maxima, silver surface plasmon resonance peak     

Estimation of the minimum machining performance in the abrasive waterjet machining using integrated ANN-SA

In this study, Artificial Neural Network (ANN) and Simulated Annealing (SA) techniques were integrated labeled as integrated ANN-SA to estimate optimal process parameters in abrasive waterjet (AWJ) machining operation. The considered process parameters include traverse speed, waterjet pressure, standoff distance, abrasive grit size and abrasive flow rate. The quality of the cutting of machined-material is assessed by looking to the roughness average value (R_a). The optimal values of the process parameters are targeted for giving a minimum value of R_a. It was evidence that integrated ANN-SA is capable of giving much lower value of R_a at the recommended optimal process parameters compared to the result of experimental and ANN single-based modeling. The number of iterations for the optimal solutions is also decreased compared to the result of SA single-based optimization.     

Genetic Algorithm and Simulated Annealing to estimate optimal process parameters of the abrasive waterjet machining

In this study, two computational approaches, Genetic Algorithm and Simulated Annealing, are applied to search for a set of optimal process parameters value that leads to the minimum value of machining performance. The objectives of the applied techniques are: (1) to estimate the minimum value of the machining performance compared to the machining performance value of the experimental data and regression modeling, (2) to estimate the optimal process parameters values that has to be within the range of the minimum and maximum coded values for process parameters of experimental design that are used for experimental trial and (3) to evaluate the number of iteration generated by the computational approaches that lead to the minimum value of machining performance. Set of the machining process parameters and machining performance considered in this work deal with the real experimental data of the non-conventional machining operation, abrasive waterjet. The results of this study showed that both of the computational approaches managed to estimate the optimal process parameters, leading to the minimum value of machining performance when compared to the result of real experimental data.     

Characterization of Clay Particle Surfaces for Contaminant Sorption in Soil Barriers Using Flow Microcalorimetry

Clays such as kaolinite and bentonite are widely used in various industries as sorbents. The sorptive characteristics of clays are exploited when they are used in contaminant barrier systems. To use clays effectively, their surface characteristics need to be known; especially, when they are used for contaminant sorption. Available surface area of clay minerals and the characteristics that depend on it are very sensitive to environmental changes such as those that can be induced by changes in the composition of pore fluid. Flow microcalorimetry with a down-stream concentration was used to determine the heats and amounts of adsorption of acids and bases on the clays. Test results presented herein revealed that both kaolinite and bentonite exhibit significantly different adsorption isotherms and heat of wetting under high pH and low pH conditions. Kaolinite has the capacity to adsorb both acidic and basic molecules almost equally. However, it has a tendency to adsorb more base than acid because of its stronger complexation capacity with acids than with bases. On the other hand, bentonite has a tendency to adsorb more acidic than basic molecules per gram. These results also indicate that both kaolinite and bentonite have different heats of wetting characteristics. As the concentrations of the acids and bases increase, the heat of wetting of kaolinite decreases while that of bentonite increases.