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1.
Blend films of two types (I and II) were prepared by mixing Antheraea mylitta silk fibroin (AMF) and gelatin solution in various blend ratios via the solution casting method. Two different crosslinkers, namely glutaraldehyde and genipin, were used during blend preparation. The structural characteristics and thermal properties of the blend films were examined by Fourier transform infrared (FTIR) spectroscopy, X‐ray diffraction (XRD), Thermogravimetric analysis (TGA) and Diffrential scanning calorimetery (DSC). The FTIR spectra showed conformational alterations in type I blend films while type II films attained high β‐sheet crystallinity. The XRD diffractograms presented a high degree of crystallinity in type II blend films compared to type I, which showed an almost amorphous structure. Further, thermal and biological studies were conducted on type II films. According to the TGA thermograms, the degradation temperature of the crosslinked blend films shifted compared to pure gelatin and pure AMF films. Partial miscibility of the two components was indicated by DSC thermograms of the blends. The high water uptake capacity of type II blend films was found to imitate hydrogel behaviour. The blend films did not show any toxicity in 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay and supported L929 fibroblast cell spreading and proliferation. The biodegradation of the blend films was significantly faster than the pure silk film. © 2020 Society of Industrial Chemistry 相似文献
2.
Haemangiopericytoma of nose and paranasal sinuses is relatively uncommon tumour. It occurs in adults in sixth and seventh decades of life. In view of paucity of intranasal haemangiopericytoma old in Indian literature and young age of patient, we are reporting one case in 28-year-old female who presented with recurrent, profuse epistaxis. 相似文献
3.
4.
RK Batra JC Olsen DK Hoganson B Caterson RC Boucher 《Canadian Metallurgical Quarterly》1997,272(18):11736-11743
Gene therapy may be an important adjuvant for treating cancer in the pleural space. The initial results of retroviral gene transfer to cancer cells in malignant pleural effusions revealed that transduction was markedly inhibited, and studies to characterize the inhibitory factor(s) were performed. The inhibition was contained within the soluble, rather than cellular, components of the effusions and was demonstrated with amphotropic, gibbon ape leukemia virus, and vesicular stomatitis virus-glycoprotein pseudotyped retroviral vectors. After excluding complement proteins, a series of studies identified chondroitin sulfates (CSs) as the inhibitory substances. First, treatment of the effusions with mammalian hyaluronidase or chondroitinases, but not Streptomyces hyaluronidase, abolished the inhibitory activity. Second, addition of exogenous CS glycosaminoglycans mimicked the inhibition observed with pleural effusions. Third, immunoassays and biochemical analyses of malignant pleural effusion specimens revealed CS in relevant concentrations within pleural fluid. Fourth, proteoglycans/glycosaminoglycans isolated from the effusions inhibited retroviral gene transfer. Analyses of the mechanism of inhibition indicate that the chondroitin sulfates interact with vector in solution rather than at the target cell surface. These results suggest that drainage of the malignant pleural effusion, and perhaps enzymatic pretreatment of the pleural cavity, will be necessary for efficient retroviral vector mediated gene delivery to pleural metastases. 相似文献
5.
We have used in vivo balloon catheterization in combination with in vitro organ culture to develop a model system for vascular neointima formation. A Fogarty balloon catheter was used to deendothelialize and rupture the internal elastic lamina of aortae in adult rabbits. After three d of recovery, aortae were harvested, divided into segments, and placed into organ culture. We obtained a daily index of cell proliferation in cultured vessels using [3H]thymidine incorporation into DNA. Also, segments were collected and processed for routine histology or immunohistochemistry. Aortic segments that had undergone ballooning 3 d before harvest and then cultured exhibited diffuse neointimal growth after several d in vitro, whereas those from sham-operated (nonballooned) rabbits showed generally only a single endothelial cell layer that is characteristic of normal intima. Aortae that were harvested, balloon-damaged in vitro, and then cultured exhibited no neointimal growth. The neointima that developed in cultured segments from in vivo ballooned rabbits was primarily of smooth muscle cell origin as determined by positive immunostaining for alpha-smooth muscle actin. The intima:media thickness ratios were significantly higher in aortic segments from ballooned rabbits at harvest and after 4 or 7 d in culture compared with those from nonballooned rabbits. Also, the [3H]thymidine index was higher in the in vivo ballooned aorta compared to non-ballooned or in vitro ballooned vessel. We conclude that ballooning in vivo followed by exposure to blood-borne elements produces an enhanced proliferative response in cultured vessels that is distinct from other in vitro models of neointimal growth. 相似文献
6.
Uma Batra S. Ray S. R. Prabhakar 《Journal of Materials Engineering and Performance》2003,12(5):597-601
A ductile iron containing 0.6% copper as the main alloying element was austempered at a fixed austempering temperature of
330 °C for a fixed austempering time of 60 min after austenitization at 850 °C for different austenitization periods of 60,
90, and 120 min. The austempering process was repeated after changing austenitization temperature to 900 °C. The effect of
austenitization temperature and time was studied on the carbon content and its distribution in the austenite after austenitization.
The effect of austenitization parameters was also studied on austempered microstructure, structural parameters like volume
fraction of austenite, X
γ
, carbon content C
γ
, and X
γ
C
γ
, and bainitic ferrite needle size, d
α
after austempering. The average carbon content of austenite increases linearly with austenitization time and reaches a saturation
level. Higher austenitization temperature results in higher carbon content of austenite. As regards the austempered structure,
the lowering austenitization temperature causes significant refinement and more uniform distribution of austempered structure,
and a decrease in the volume fraction of retained austenite. 相似文献
7.
Almuatasim Alomari Ashok Batra Mohan Aggarwal C. R. Bowen 《Journal of Materials Science: Materials in Electronics》2016,27(10):10020-10030
This paper demonstrates a multi-source energy harvester that is able to utilize simultaneously both piezoelectric and pyroelectric effects in lead magnesium niobate-lead titanate (PMN-PT) single crystal. The paper presents a study of PMN-PT single crystal with a (67:33) composition grown in our laboratory via a vertical gradient freeze method without any flux. The performance of the piezoelectric and pyroelectric energy harvester using unimorph device structure was evaluated via modeling and experiment. The theoretical study was implemented based on a distributed parameter electromechanical model and the modelling procedure was approximated using finite element analysis to predict the electromechanical behavior of the harvester. The maximum power density at a resonance frequency of 50 Hz and optimum resistance of 2 MΩ was 16.7 nW/(g2 cm3) under a 1 g acceleration of vibration. The measured values of electrical output parameters were in good agreement with theoretical and modelling results using MATLAB and COMSOL Multiphysics, respectively. By using the pyroelectric effect along with the piezoelectric effect, the output voltage of the energy harvester was found to be enhanced at the optimum resistance and specific frequency values. It was noticed that the output voltage was increased monotonically with temperature-difference (ΔT) and reaches up to 180 % of its original value under temperature difference of 1.7 °C at a frequency value of 49 Hz. 相似文献
8.
A.J.M. Ferreira R.C. Batra C.M.C. Roque L.F. Qian R.M.N. Jorge 《Composite Structures》2006,75(1-4):593-600
We use the global collocation method, the first and the third-order shear deformation plate theories, the Mori–Tanaka technique to homogenize material properties, and approximate the trial solution with multiquadric radial basis functions to analyze free vibrations of functionally graded plates. Frequencies computed by the present method are found to agree well with those from the analytical solution of Vel and Batra, and the numerical solution of Qian et al. based on the meshless local Petrov–Galerkin formulation. 相似文献
9.
Optimization of process parameters is helpful in efficient working of the process and, hence, in lowering the cost of machining. Optimization of ECM process parameters has been achieved by considering only one objective at a time from metal removal rate, geometrical accuracy, and total process cost. From a practical point of view, a solution of the ecm problem satisfying all three objectives simultaneously is highly desirable.In the proposed model, a multi-objective problem involving the ecm process is formulated producing highly nonlinearized equations. These are then linearized by regression analysis and converted into a goal programming format. Finally, the problem is solved by the partitioning algorithm.It is concluded that the tool, or cathode, remains safe at the optimal values of design variables obtained in the examples discussed. The optimal value of voltage when metal removal rate is the only objective, is found to be higher than the case when the geometrical accuracy requirement is also to be satisfied. 相似文献
10.
Afshin Moradi Harsh Sharma Ravi Datta Sharma Achala Fernando Roberto A. Barrero Jyotsna Batra 《International journal of molecular sciences》2022,23(20)
Single nucleotide polymorphisms (SNPs) impacting the alternative splicing (AS) process (sQTLs) or isoform expression (iso-eQTL) are implicated as important cancer regulatory elements. To find the sQTL and iso-eQTL, we retrieved prostate cancer (PrCa) tissue RNA-seq and genotype data originating from 385 PrCa European patients from The Cancer Genome Atlas. We conducted RNA-seq analysis with isoform-based and splice event-based approaches. The MatrixEQTL was used to identify PrCa-associated sQTLs and iso-eQTLs. The overlap between sQTL and iso-eQTL with GWAS loci and those that are differentially expressed between cancer and normal tissue were identified. The cis-acting associations (FDR < 0.05) for PrCa-risk SNPs identified 42, 123, and 90 PrCa-associated cassette exons, intron retention, and mRNA isoforms belonging to 25, 95, and 83 genes, respectively; while assessment of trans-acting association (FDR < 0.05) yielded 59, 65, and 196 PrCa-associated cassette exons, intron retention and mRNA isoforms belonging to 35, 55, and 181 genes, respectively. The results suggest that functional PrCa-associated SNPs can play a role in PrCa genesis by making an important contribution to the dysregulation of AS and, consequently, impacting the expression of the mRNA isoforms. 相似文献