排序方式: 共有38条查询结果,搜索用时 15 毫秒
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Alfredo M. Gravagnuolo Eden Morales‐Narváez Charlene Regina Santos Matos Sara Longobardi Paola Giardina Arben Merkoçi 《Advanced functional materials》2015,25(38):6084-6092
Class I hydrophobin Vmh2, a peculiar surface active and versatile fungal protein, is known to self‐assemble into chemically stable amphiphilic films, to be able to change wettability of surfaces, and to strongly adsorb other proteins. Herein, a fast, highly homogeneous and efficient glass functionalization by spontaneous self‐assembling of Vmh2 at liquid–solid interfaces is achieved (in 2 min). The Vmh2‐coated glass slides are proven to immobilize not only proteins but also nanomaterials such as graphene oxide (GO) and quantum dots (QDs). As models, bovine serum albumin labeled with Alexa 555 fluorophore, anti‐immunoglobulin G antibodies, and cadmium telluride QDs are patterned in a microarray fashion in order to demonstrate functionality, reproducibility, and versatility of the proposed substrate. Additionally, a GO layer is effectively and homogeneously self‐assembled onto the studied functionalized surface. This approach offers a quick and simple alternative to immobilize nanomaterials and proteins, which is appealing for new bioanalytical and nanobioenabled applications. 相似文献
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Nano Research - The widespread and increasing interest in enhancing biosensing technologies by increasing their sensitivities and lowering their costs has led to the exploration and application of... 相似文献
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Electrocatalytic Detection: Magnetic Bead/Gold Nanoparticle Double‐Labeled Primers for Electrochemical Detection of Isothermal Amplified Leishmania DNA (Small 2/2016) 下载免费PDF全文
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Pérez B Pumera M del Valle M Merkoçi A Alegret S 《Journal of nanoscience and nanotechnology》2005,5(10):1694-1698
A novel glucose biosensor based on a rigid and renewable carbon nanotube (CNT) based biocomposite is reported. The biosensor was based on the immobilization of glucose oxidase (GOx) within the CNT epoxy-composite matrix prepared by dispersion of multi-wall CNT inside the epoxy resin. The use of CNT, as the conductive part of the composite, ensures better incorporation of enzyme into the epoxy matrix and faster electron transfer rates between the enzyme and the transducer. Experimental results show that the CNT epoxy composite biosensor (GOx-CNTEC) offers an excellent sensitivity, reliable calibration profile, and stable electrochemical properties together with significantly lower detection potential (+0.55 V) than GOx-graphite epoxy composites (+0.90 V; difference deltaE = 0.35 V). The results obtained favorably compare to those of a glucose biosensor based on a graphite epoxy composite (GOx-GEC). 相似文献
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Double-codified gold nanolabels for enhanced immunoanalysis 总被引:7,自引:0,他引:7
Ambrosi A Castañeda MT Killard AJ Smyth MR Alegret S Merkoçi A 《Analytical chemistry》2007,79(14):5232-5240
A novel double-codified nanolabel (DC-AuNP) based on gold nanoparticle (AuNP) modified with anti-human IgG peroxidase (HRP)-conjugated antibody is reported. It represents a simple assay that allows enhanced spectrophotometric and electrochemical detection of antigen human IgG as a model protein. The method takes advantage of two properties of the DC-AuNP label: first, the HRP label activity toward the OPD chromogen that can be related to the analyte concentration and measured spectrophotometrically; second, the intrinsic electrochemical properties of the gold nanoparticle labels that being proportional to the protein concentration can be directly quantified by stripping voltammetry. Beside these two main direct determinations of human IgG, a secondary indirect detection was also applicable to this system, exploiting the high molar absorptivity of gold colloids, by which, the color intensity of their solution was proportional to the concentration of the antigen used in the assay. Paramagnetic beads were used as supporting material to immobilize the sandwich-type immunocomplexes resulting in incubation and washing times shorter than those typically needed in classical ELISA tests by means of a rapid magnetic separation of the unbound components. A built-in magnet graphite-epoxy-composite electrode allowed a sensibly enhanced adsorption and electrochemical quantification of the specifically captured AuNPs. The used DC-AuNP label showed an excellent specificity/selectivity, as a matter of fact using a different antigen (goat IgG) a minimal nonspecific electrochemical or spectrophotometric signal was measured. The detection limits for this novel double-codified nanoparticle-based assay were 52 and 260 pg of human IgG/mL for the spectrophotometric (HRP-based) and electrochemical (AuNP-based) detections, respectively, much lower than those typically achieved by ELISA tests. The developed label and method is versatile, offers enhanced performances, and can be easily extended to other protein detection schemes as well as in DNA analysis. 相似文献
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Eden Morales‐Narváez Luis Baptista‐Pires Alejandro Zamora‐Gálvez Arben Merkoçi 《Advanced materials (Deerfield Beach, Fla.)》2017,29(7)
The main properties of graphene derivatives facilitating optical and electrical biosensing platforms are discussed, along with how the integration of graphene derivatives, plastic, and paper can lead to innovative devices in order to simplify biosensing technology and manufacture easy‐to‐use, yet powerful electrical or optical biosensors. Some crucial issues to be overcome in order to bring graphene‐based biosensors to the market are also underscored. 相似文献
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Eden Morales‐Narváez Arben Merkoçi 《Advanced materials (Deerfield Beach, Fla.)》2012,24(25):3298-3308
Since graphene exhibits innovative mechanical, electrical, thermal, and optical properties, this 2D material is increasingly attracting attention and is under active research. Among the various graphene forms with lattice‐like nanostructure, graphene oxide (GO) displays advantageous characteristics as a biosensing platform due to its excellent capabilities for direct wiring with biomolecules, a heterogeneous chemical and electronic structure, the possibility to be processed in solution and the ability to be tuned as insulator, semiconductor or semi‐metal. Moreover, GO photoluminescences with energy transfer donor/acceptor molecules exposed in a planar surface and is even proposed as a universal highly efficient long‐range quencher, which is opening the way to several unprecedented biosensing strategies. Here, the rationale behind the use of GO in optical biosensing applications is discussed by describing different potentially exploitable properties of GO, and an overview of the current approaches are presented along with future perspectives and challenges. 相似文献