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排序方式: 共有200条查询结果,搜索用时 31 毫秒
1.
VP Korhonen M Tolvanen JM Hyttinen M Uusi-Oukari R Sinervirta L Alhonen M Jauhiainen OA J?nne J J?nne 《Canadian Metallurgical Quarterly》1997,245(2):482-489
We have generated several transgenic mouse lines and rabbits expressing efficiently (up to 0.3 mg/ml in mice and up to 0.5 mg/ml in rabbits) human erythropoietin in their milk as bovine beta-lactoglobulin fusion protein. Human erythropoietin cDNA was inserted in frame into exon 5 of the bovine beta-lactoglobulin gene with a linker oligonucleotide encoding the cleavage site for bacterial IgA protease. RNA analysis performed on one lactating transgenic mouse and one transgenic rabbit revealed that the fusion gene was expressed almost exlusively in the mammary gland, although low amounts of transgene-derived RNA were detectable in salivary glands and uterus or in the kidney. The fusion protein was specifically cleaved with IgA protease. The erythropoietin part obtained upon digestion had a lower molecular mass than recombinant erythropoietin produced in Chinese hamster ovary cells. By deglycosylation analysis it was shown that the difference in size was due to a different type of glycosylation. Biological activity of the fusion protein, as determined by growth stimulation of TF-1 erythroleukemia cells, was less than 15% of that of human recombinant erythropoietin. Upon digestion of the fusion protein with IgA protease, biological activity comparable to that of the recombinant erythropoietin was recovered. Transgenic males and virgin females did not show signs of enhanced erythropoiesis, but lactating females expressing the transgene displayed transient increases in their hematocrit values. 相似文献
2.
The interaction between negative ions and
3
He superfluids was studied by using a time-of-flight spectrometer having a 10–3 relative resolution. The ions guided through a sample of rotating3He-A were found to be focused into the cores of the vortices present in this rotating superfluid. Two different types, with different core structures and presumably different numbers of circulation quanta associated with them, were found. The ion mobility along the vortex cores,
c
, observed in the absence of external magnetic field, can be explained by the mobility anisotropy of the A phase, which indicates that the vortices are continuous, i.e., consist of the A phase even in the core. In finite magnetic fields, a qualitatively similar continuous vortex structure was found, a result compatible with the earlier nuclear magnetic resonance (NMR) experiments, but also a new vortex type with an anomalously high
c
was seen when the vortex sample was prepared adiabatically. The observed strong ion focusing indicates broken w-symmetry in the core textures. This is in contradiction with the earlier numerical calculations on the optimal continuous vortex texture. Experiments to observe trapping of ions into the vortex cores were performed both in A and B phases. No trapping could be detected. Mobility measurements in stationary liquid were extended down toT=0.3T
c
. Comparison with the calculated transport coefficients yields
A
(T)=1.32
BCS
(T) for the maximal A phase gap atP=29.3 bar, and
B
(T)=1.12
BCS
(T) for the B phase gap at 4.8 bar. In the normal Fermi liquid a shallow minimum of ion mobility was detected aroundT2T
c
=5 mK. 相似文献
3.
Material characterization has become increasingly important with the adoption of simulation‐based workflow for microwave electronics design. This article focuses on the characterization of printable electronics materials using transmission‐line measurements and demonstrates the capability of the multiline characterization method to separate dielectric and conductor losses when line geometry and material properties vary between the lines. The effects of multiline algorithm, number of line standards, and methods for selecting optimal line lengths are demonstrated. Consistent characterization results are obtained from inkjet‐printed transmission lines on two different substrate materials. In addition, local conductor thickness variations are demonstrated as an effective way to decrease losses. Finally, the simulation‐based procedure for determining the material properties is outlined and applied to printable electronics characterization. © 2013 Wiley Periodicals, Inc. Int J RF and Microwave CAE 24:177–183, 2014. 相似文献
4.
Heikki Ailisto Lauri Pohjanheimo Pasi Välkkynen Esko Strömmer Timo Tuomisto Ilkka Korhonen 《Personal and Ubiquitous Computing》2006,10(6):333-344
The prevalent visions of ambient intelligence emphasise natural interaction between user and functions and services embedded in the environment or available through mobile devices. In these scenarios the physical and virtual worlds seamlessly gear into each other, making crossing the border between these worlds natural or even invisible to the user. The bottleneck in reaching these scenarios appear in the natural mapping between the physical objects and their virtual counterparts. The emergence of local connectivity in mobile devices opens possibilities for implementing novel user interface paradigms to enhance this mapping. We present physical selection paradigm for implementing an intuitive human technology interaction for mobile devices. In order to demonstrate the feasibility of the paradigm we implemented two experimental set-ups using commercially available smart phones with IrDA connectivity. The experiments involved selecting a website by physically pointing at its symbol and making a phone call by pointing at an icon representing the person to be called. In tentative user experiments the physical selection method was more time-efficient and it was perceived more positively by the users than a conventional method.
相似文献
Heikki AilistoEmail: |
5.
Harri Tuomisto Petra Lundstrm Ritva Korhonen Jzsef Elter Milan Hladký Jozef Tomek Ludovít Fagula 《Nuclear Engineering and Design》2003,221(1-3):181
There are twelve operating VVER-440/213 reactors in the Czech Republic, Hungary and Slovakia. The plant owners have carried out extensive safety enhancement programmes, which have included measures to improve prevention, mitigation and control of design basis accidents. Recently, there have been a number of projects to study the Severe Accident Management aspects of these plants. The plant owners have also initiated Plant Life Management programmes. The objective of the VERSAFE Concerted Action is to create a network of the VVER-440/213 plant owners and operators, which aims at definition of the further research needs of severe accidents and plant ageing from the utilities’ viewpoint. The starting point is to formulate a general approach to severe accident management and plant life management and then proceed to define the needs for the additional information expected from the safety research. The role of national research institutes and organisations of the partner countries is of crucial importance in performing such research and, thus, creating and maintaining the expertise on the national level. The successful completion of the project will assist in establishing well-defined research projects that are oriented to the needs of the end-users and provide information that can be used to facilitate and support the negotiations of the EU applicant countries operating VVER-440 reactors. 相似文献
6.
Angiotensin I-converting enzyme inhibitory properties of whey protein digests: concentration and characterization of active peptides 总被引:11,自引:0,他引:11
Pihlanto-Leppälä A Koskinen P Piilola K Tupasela T Korhonen H 《The Journal of dairy research》2000,67(1):53-64
The aim of this study was to identify whey-derived peptides with angiotensin I-converting enzyme (ACE) inhibitory activity. The bovine whey proteins alpha-lactalbumin and beta-lactoglobulin were hydrolysed with pepsin, trypsin, chymotrypsin, pancreatin, elastase or carboxypeptidase alone and in combination. The total hydrolysates were fractionated in a two step ultrafiltration process, first with a 30 kDa membrane and then with a 1 kDa membrane. Inhibition of ACE was analysed spectrophotometrically. The peptides were isolated by chromatography and identified by mass and sequencing analysis. The most potent inhibitory peptides were synthesized by the 9-fluorenylmethoxycarbonyl solid phase method. Inhibition of ACE was observed after hydrolysis with trypsin alone, and with an enzyme combination containing pepsin, trypsin and chymotrypsin. Whey protein digests gave a 50% inhibition (IC50) of ACE activity at concentration ranges within 345-1733 micrograms/ml. The IC50 values for the 1-30 kDa fractions ranged from 485 to 1134 micrograms/ml and for the < 1 kDa fraction from 109 to 837 mg/ml. Several ACE-inhibitory peptides were isolated from the hydrolysates by reversed-phase chromatography, and the potencies of the purified peptide fractions had IC50 values of 77-1062 microM. The ACE-inhibitory peptides identified were alpha-lactalbumin fractions (50-52), (99-108) and (104-108) and beta-lactoglobulin fractions (22-25), (32-40), (81-83), (94-100), (106-111) and (142-146). 相似文献
7.
Nokia Miriam S.; Penttonen Markku; Korhonen Tapani; Wikgren Jan 《Canadian Metallurgical Quarterly》2009,123(3):631
The authors examined the relationship between hippocampal theta activity and trace eyeblink conditioning. Hippocampal electrophysiological local field potentials were recorded before, during, and after conditioning or explicitly unpaired training sessions in adult male New Zealand White rabbits. As expected, a high relative power of theta activity (theta ratio) in the hippocampus predicted faster acquisition of the conditioned response during trace conditioning but, contrary to previous results obtained using the delay paradigm, only in the initial stage of learning. The presentation of the conditioned stimulus overall elicited an increase in the hippocampal theta ratio. The theta ratio decreased in the unpaired group as a function of training, remained high throughout conditioning in the fast learners, and rapidly increased in the slow learners initially showing a low theta ratio. Our results indicate a reciprocal connection between the hippocampal oscillatory activity and associative learning. The hippocampal theta ratio seems to reflect changes and differences in the subjects’ alertness and responsiveness to external stimuli, which affect the rate of learning and are, in turn, affected by both conditioning and unpaired training. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
8.
The objective of this study was to determine a suitable treatment method for variable waters from a forest industry landfill site. The main target was to find out the impact of different chemical treatments on the composition and biodegradability of those waters. Earlier studies have shown that biological treatment alone is not a suitable treatment method for these waters. That is why ozonation, ozonation+hydrogen peroxide and hydrogen peroxide treatment were studied in a laboratory scale. The ozonated waters were also biologically post-treated.
All the methods studied were able to degrade a part of the organic compounds and convert them into a more biodegradable form. Also the BOD/COD -ratio increased significantly. The removal of organic compounds by ozonation was 30 - 50 %. Hydrogen peroxide addition did not improve the degradation. The combination of pre-ozonation and biological post-treatment gave a total TOC removal between 50 - 95 %. 相似文献
9.
Eero Hyvnen Eetu Mkel Mirva Salminen Arttu Valo Kim Viljanen Samppa Saarela Miikka Junnila Suvi Kettula 《Journal of Web Semantics》2005,3(2-3):224
This article presents the semantic portal MuseumFinland for publishing heterogeneous museum collections on the Semantic Web. It is shown how museums with their semantically rich and interrelated collection content can create a large, consolidated semantic collection portal together on the web. By sharing a set of ontologies, it is possible to make collections semantically interoperable, and provide the museum visitors with intelligent content-based search and browsing services to the global collection base. The architecture underlying MuseumFinland separates generic search and browsing services from the underlying application dependent schemas and metadata by a layer of logical rules. As a result, the portal creation framework and software developed has been applied successfully to other domains as well. MuseumFinland got the Semantic Web Challence Award (second prize) in 2004. 相似文献
10.
Korhonen I Karhu J Mainardi L Jakob SM 《Computer methods and programs in biomedicine》2000,63(3):211-218
Measuring effects of sensory stimuli on haemodynamics could provide information about the interplay between central and autonomous nervous system (ANS). However, ANS response to sensory stimulus has received little attention. In this paper we present a signal processing scheme to extract the responses of heart rate and systemic arterial pressure on auditory stimulus in intensive care patients (N=5). In short, the effect of mechanical ventilation is rejected by optimal linear modelling. Other disturbances are attenuated by filtering and efficient rejection of outlying sweeps of data. The results show identifiable responses on three out of five cases. The response characteristics may be explained by synchronisation of spontaneous variability in systemic arterial pressure to auditory stimulus. 相似文献