As the outermost barrier of the body, skin is a major target of oxidative stress. In the brain, estrogen has been reported synthesized locally and protects neurons from oxidative stress. Here, we explored whether estrogen is also locally synthesized in the skin to protect from oxidative stress and whether aberrant local estrogen synthesis is involved in skin disorders. Enzymes and estrogen receptor expression in skin cells were examined first by quantitative real-time PCR and Western blot analyses. Interestingly, the estrogen synthesis enzyme was mainly localized in epidermal keratinocytes and estrogen receptors were mainly expressed in melanocytes among 13 kinds of cultured human skin cells. The most abundant estrogen synthesis enzyme expressed in the epidermis was 17β-hydroxysteroid dehydrogenase 1 (HSD17β1) localized in keratinocytes, and the most dominant estrogen receptor expressed in the epidermis was G protein-coupled estrogen receptor 1 (GPER1) in melanocytes. To investigate whether keratinocyte-derived estradiol could protect melanocytes from oxidative stress, cultured human primary epidermal melanocytes (HEMn-MPs) were treated with H2O2 in the presence or absence of 17β estradiol or co-cultured with HSD17β1 siRNA-transfected keratinocytes. Keratinocyte-derived estradiol exhibited protective effects against H2O2-induced cell death. Further, reduced expression of HSD17β1 in the epidermis of skin from vitiligo patients was observed compared to the skin from healthy donors or in the normal portions of the skin in vitiligo patients. Our results suggest a possible new target for interventions that may be used in combination with current therapies for patients with vitiligo. 相似文献
Summary CP/DD/MASS 13C-NMR spectra have been obtained for regenerated cellulose samples with different crystallinities as well as for cotton, -D-glucose, -D-cellobiose, and cellopentaose. The spectra of the regenerated cellulose samples exhibit broad multiplicities of the C-4 and C-6 resonance lines in a similar manner as those of native cellulose samples such as cotton and ramie, and, in addition, another broad tailing of the C-1 resonance. Since these multiplicities change linearly with crystallinity, it is concluded that they are ascribed to the contributions from the crystalline and noncrystalline components. Effects of hydrogen bonds and conformations of the -1,4-glycosidic linkage on the chemical shifts are also discussed. 相似文献
This study aimed at investigating the effect of drying conditions on spaghetti properties, i.e., its color, surface structure, rupture strength, rehydration characteristics, texture, and sauce retention capacity. The effects of temperature and humidity were independently examined under constant drying conditions, which were compared to those applied industrially, where the temperature and relative humidity are changed stepwise with time. The knowledge obtained in this study is considered useful for reasonably determining the drying conditions for producing spaghetti with desired properties. 相似文献
This paper focuses on temperature rise due to the viscous dissipation in liquids flowing through micro-channels. In the past, equations for the prediction of the temperature rise have been obtained as a function of the friction factor, Reynolds number and Eckert number or a similar form, starting from Navier–Stokes equation and energy equation under the assumption of fully developed laminar flow by researchers. The temperature rises calculated from the equations have been compared with experimental data and the equations have been validated. However, in this paper, a new equation for the prediction of the temperature rise is simply obtained from the first law of thermodynamics without restriction of fully developed laminar flow. 相似文献
Mild and simple catalytic systems consisting of molybdenum(VI) dichloride dioxide [MoO2Cl2] as a catalyst and a phosphine as reductant have been developed for the stereospecific deoxygenation of epoxides to alkenes. The reactions using 1,2‐bis(diphenylphosphino)ethane (dppe) and triphenylphosphine (PPh3) proceed with retention and inversion of stereochemistry, respectively. The mild reaction tolerates the presence of various functional groups and affords stereodefined substituted olefins in good yields.
An investigation was undertaken to establish the concentration in paper products of dehydroabietic (DHA) and abietic (AA) resin acids, present in rosin, which are major toxicants of pulp- and paper-mill effluent. Their migration was studied from paper and paperboard products into various food-simulating solvents and the substitute fatty food simulant Tenax TA (modified polyphenylene oxide). DHA and AA were detected in five of 10 virgin paper products and in all 10 recycled paperboard products for food-contact use at concentrations of 14-500 and 110-1200 µg/g, respectively. In virgin paper products, the highest migration was into 95% ethanol or heptane, with negligible or no migration into other solvents. In recycled paperboard products, migration was highest into 95% ethanol, but was also observed into 20% ethanol, water and heptane. Migration to Tenax TA was also observed and the migration level increased with time. The maximum migration levels of DHA and AA into food simulants were 0.853 and 3.14 µg/g, respectively. The results suggest that, in the worst case, the daily intake of DHA and AA from paper and paperboard products was 50 times lower than the tolerable daily intake of rosin. 相似文献
Infants, whose growth and development are highly dependent upon the endocrine system, are particularly vulnerable to endocrine disruptor exposure. In our study, we examined whether or not alkylphenols, bisphenol A, benzophenone, di(2-ethylhexyl)adipate and 10 phthalates are present in containers of baby food. Furthermore, the migration into food-simulating solvents was also examined. Sixteen types of containers of baby food, obtained on the Japanese market in 2001, were examined: 8 glass bottle types, 1 plastic type, 1 metallic package (laminated with plastic) and 6 multilayered laminated film packages. Alkylphenols, benzophenone, bisphenol A and di(2-ethylhexyl)adipate were not detected in any of the samples. On the other hand, dibutylphthalate (DBP) was detected in 7 samples (1.2-9.1 micrograms/sample), and di(2-ethylhexyl)phthalate (DEHP) was detected in 15 samples (1.3-18 micrograms/sample). Migration tests were carried out for 5 samples, and the levels of DBP and DEHP that migrated into n-heptane were 0.68-1.7 micrograms/sample and 0.33-3.5 micrograms/sample, respectively. From these results, a baby's intake levels of DBP and DEHP were estimated to be about 1/2,500 and 1/90-300 of the tolerable daily intake (TDI), respectively. 相似文献
Numerical solutions are obtained for natural convection heat transfer in an open channel with corrugated, isothermal confining walls. The channel is very long so that the fluid temperature approaches the wall temperature and the flow can be assumed to be periodically fully developed. The solutions are obtained by solving the full elliptic governing equations in a transformed coordinate system which maps the channel with corrugated walls onto a channel with flat walls. The periodic, fully developed Nusselt number for the corrugated channel is expressed by the relation Nu = CGrPr/(L/W) where Gr, Pr and L/W are the Grashof number, the Prandtl number and the aspect ratio, respectively, and C is a parameter which is a function of Gr, L/W and the corrugation angle θ. In the limiting case of θ = 0° (two flat walls), the parameter C approaches a constant value. This value is within 1.6% of the exact analytical result. 相似文献