The effect of polyethylene crystallinity and polarity on thermal stability and controlled release of essential oils in antimicrobial films

Antimicrobial packaging can preserve and increase shelf life of free preservatives food products. Active materials present in the packaging material can migrate, in a controlled manner, to the food surface, avoiding bacterial and fungal proliferation and keeping the food product edible for longer periods of time. Essential oils (EO) are natural antimicrobial agents that can be released to the headspace with no direct contact between the package and the food. To minimize loses of EO during high heat melt processing, a three stages process was implemented and tested. Antimicrobial films were prepared by melt mixing a variety of polyethylene copolymers in the presence of organo‐modified montmorillonite nano clay (NC) and thymol, an EO present in oregano and thyme. A controlled EO desorption from films can be achieved by changing the polymer crystallinity and polarity. As the crystallinity increased, the thermal stability of the EO during the extrusion process improved. The addition of NC affects the structure and homogeneity of the crystals. The combination of high polymer crystallinity and chemical affinity between EO and NC increased the thermal stability of the EO during film processing, enabling to control the desorption rate. The effect of multilayer structure based on varied densities and polarities was also studied. Increasing the polarity of the outer layers in multilayered film reduced the EO desorption rate as a result of chemical interactions between the polymer and the EO. The final antimicrobial activity of the films was also found to be dependent on the EO partitioning. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40309.     

Active contours: Generalization of the snake mode

Abstract— Segmentation is one of the fundamental issues in the field of image processing and computer vision. Various approaches include differentiating an object in the image as a final goal or for further processing (medical diagnosis, surveillance, 3‐D reconstruction and more). Snakes, a model proposed by Kass, Witkin, and Terzopoulos in 1987, provides an efficient method for segmenting an object through the minimization of its energy. The advantage of snakes is in its ability to use high‐level data given by the algorithm operator, as opposed to other methods such as the Laplace technique. The snakes model inherently imposes strong constraints on a given image in order to successfully segment an object. In this paper, the use of adjustment methods is described, which allow us to generalize the snake model to a wider range of applications. Through the use of pre‐processing techniques, the model's constraints were softened. The main theoretical model and its use in facing a real life image is presented.     

Surface Oxidation as a Cause of High Open‐Circuit Voltage in CdSe ETA Solar Cells

TiO₂/CdSe/CuSCN extremely thin absorber (ETA) solar cells are found to give relatively high values of open‐circuit voltage (>0.8 V) but low currents upon annealing the cadmium selenide (CdSe) in air (500 ºC). Annealing in N₂ produces much lower photovoltages and slightly lower photocurrents. Band structure measurements show differences between the two annealing regimes that, however, appear to favor the N₂‐annealed CdSe. On the other hand, chemically resolved electrical measurements (CREM) of the cells reveal marked differences in photo‐induced charge trapping, in particular at absorber grain boundaries of the air versus N₂‐annealed systems, correlated with the formation of Cd–O species at the CdSe surface. Using transient absorption and photovoltage decay, pronounced lifetime differences are also observed, in agreement with the strong suppression of charge recombination. The results point to a multiple role of grain surface‐oxidation, which both impedes electron injection from the CdSe to the TiO_2, but, much more significantly, enhances hole injection to the CuSCN via passivation of hole traps that act as efficient recombination centers.     

Blocking the PCNA/NKp44 Checkpoint to Stimulate NK Cell Responses to Multiple Myeloma

Simple SummaryMembrane-associated PCNA is expressed on the surface of human MM cell lines and primary MM cells. Mab 14-25-9 interacts with membrane-associated PCNA and blocks its binding to NK-expressed NKp44, thus activating NK function. We showed that mAb 14-25-9 can serve as an immune checkpoint blocker, enhancing the function of NK cells on target human MM cell lines and primary cells.AbstractMultiple Myeloma (MM) is a devastating malignancy that evades immune destruction using multiple mechanisms. The NKp44 receptor interacts with PCNA (Proliferating Cell Nuclear Antigen) and may inhibit NK cells’ functions. Here we studied in vitro the expression and function of PCNA on MM cells. First, we show that PCNA is present on the cell membrane of five out of six MM cell lines, using novel anti-PCNA mAb developed to recognize membrane-associated PCNA. Next, we stained primary bone marrow (BM) mononuclear cells from MM patients and showed significant staining of membrane-associated PCNA in the fraction of CD38⁺CD138⁺ BM cells that contain the MM cells. Importantly, blocking of the membrane PCNA on MM cells enhanced the activity of NK cells, including IFN-γ-secretion and degranulation. Our results highlight the possible blocking of the NKp44-PCNA immune checkpoint by the mAb 14-25-9 antibody to enhance NK cell responses against MM, providing a novel treatment option.     

Adaptation of the program for positioning direction of handling robot to working space for installation during systematic plane errors

The paper presents a method to compensate for the systematic plane error of a handling robot while executing installation operations of the flat installation type of plane objects or box covers with small overlap height. The method may also be used in the welding and cutting of plane contours. The systematic error is found on the basis of position measurements at three points, and the necessary correction of the program is calculated. Thus, by the start of the production process, the robot positions the objects exactly or executes the contour operations with high precision.     

The nuclear magnetic susceptibility of dilute solutions of3He in4He at low temperatures

A theory of the nuclear magnetic susceptibility of dilute solutions of ³ He in ⁴ He at low temperatures is presented. It is shown that ^–1 is a linear function of the corresponding inverse free fermion magnetic susceptibility. It is also shown that the experimental Curie constant differs from the theoretical one by an amount which is of the order of the ³ He concentration. It follows that the existing experimental results are not adequate for the calculation of the Fermi liquid magnetic susceptibility enhancement factor.     

Laser-Based Printing: From Liquids to Microstructures

Assembly of materials into microstructures under laser guidance is attracting wide attention. The ability to pattern various materials and form 2D and 3D structures with micron/sub-micron resolution and less energy and material waste compared with standard top-down methods make laser-based printing promising for many applications, for example medical devices, sensors, and microelectronics. Assembly from liquids provides a smaller feature size than powders and has advantages over other states of matter in terms of relatively simple setup, easy handling, and recycling. However, the simplicity of the setup conceals a variety of underlying mechanisms, which cannot be identified simply according to the starting or resulting materials. This progress report surveys the various mechanisms according to the source of the material—preformed or locally synthesized. Within each category, methods are defined according to the driving force of material deposition. The advantages and limitations of each method are critically discussed, and the methods are compared, shedding light on future directions and developments required to advance this field.     

Three-dimensional shape recognition using computer-generated holograms and temporal light-in-flight technique

Visualization of light propagation and light in flight are general names for viewing a pulse of light traveling through an optical system. Abramson suggested [Appl. Opt. 30, 1242 (1991)] the use of light-in-flight techniques for holographic comparison of different objects. His system is based on sending picosecond pulses in such a sequence that, if the object has the desired shape, all the scattered light arrives simultaneously at an ultrafast detector. The result is that the shortness of the detected pulses is a measure of the similarity between a holographically recorded master object and the test object. The reference hologram is recorded from a master surface, which is not always available. This method suffers from low-contrast results. A computer-generated-hologram technique is suggested and mathematically analyzed. This technique overcomes the low-contrast problem, and a master object is not needed.     

Designing and testing a calibrating procedure for combining the coordination systems of a handling robot and a stationed video camera

The paper discusses some of the results of designing and testing a calibrating procedure with important implications in contemporary robotics. This procedure is also mentioned in the literature as the “procedure for calibrating by matching the coordination systems of a robot and a stationed video camera”. The procedure is tested by a training robotechnic system, which consists of an anthropomorphic robot with five stages of freedom and a stationed video camera that can identify solid stationary objects. The results of this experimental testing are used to create and adopt some new methods for laboratory exams in robotics for students of FA in a Technical University and for developing further research on calibrating procedures in robotics needed for the industry.