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排序方式: 共有204条查询结果,搜索用时 15 毫秒
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2.
Winterhalter M. Klotz K.-H. Benz R. Arnold W.M. 《Industry Applications, IEEE Transactions on》1996,32(1):125-130
Electric field methods are powerful tools for cell characterization and manipulation. Important biotechnological applications, e.g., electrofusion of cells or electroinjection of macromolecules into living cells, rely on membrane breakdown. Despite the widespread use of these techniques, the process of membrane breakdown is poorly understood: a better understanding can be expected to lead to higher efficiencies. The present study used planar lipid bilayers in order to limit the number of experimental parameters. Membrane rupture was induced by careful application of short electric field pulses: measurement of the subsequent increase in membrane conductivity with time permitted the underlying mechanism to be characterized. The initial process of pore formation starts a few μs after the onset of the pulse, and the ensuing breakdown of the entire lipid membrane occurs within about a millisecond (pore widening velocity of a few cm/s). The kinetics of the pore-opening process could be influenced qualitatively by adding surfactants and lipid-attached macromolecules, respectively. Changing the effective mass per unit area of membrane, which could be brought about by using known percentages of lipids with covalently bound, hydrophilic polymers, allowed a model for the pore widening process to be quantitatively tested 相似文献
3.
C Benz WR Martin J Arnold R Jakobs JF Riemann 《Canadian Metallurgical Quarterly》1997,122(13):391-395
OBJECTIVE: To evaluate whether push enteroscopy of the small bowel (PES), undertaken after extensive previous investigations in suspected intestinal bleeding from an uncertain site, chronic diarrhoea or lymphoma of the small intestine, contributes to the diagnosis, and to ascertain the results of PES and its clinical significance. PATIENTS AND METHODS: 56 consecutive patients (29 men, 27 women; mean age 63 years) were investigated prospectively. The main indications for PES were the search for the source of intestinal bleeding in 79% of patients (group A), chronic diarrhoea or tropical sprue in 16% (group B) and search for tumour of lymphoma in 5% (group C). PES was always performed in fasting patients under sedation/analgesia using a video PES, which contrary to catheter enteroscopy provides a channel for intervention. RESULTS: In group A 27% of patients were found to have lesions, in particular angiodysplasias, or (in once case) leiomyoma. Half of these patients were successfully treated endoscopically without later surgical intervention being required (mean follow-up of six months). In the other half operation became necessary, either because the disease itself indicated it or the bleeding persisted, the source being in the more distant small intestine and thus not accessible to endoscopic intervention: only 50% of the length of the small intestine proved to be within reach of the instrument. No abnormalities were discovered in patients of groups B and C. CONCLUSION: In cases of gastrointestinal bleeding from an uncertain source PES should be performed first, because in many cases it may obviate surgical intervention. But PES seems to contribute little of diagnostic value in other indications. 相似文献
4.
The effect of a permanent transection on myelin gene expression in a regenerating sciatic nerve and in an adult sciatic nerve was compared to establish the degree of axonal control exerted upon Schwann cells in each population. First, the adult sciatic nerve was crushed, and the distal segment allowed to regenerate. At 12 days post-crush, the sciatic nerve was transected distal to the site of crush to disrupt the Schwann cell-axonal contacts that had reformed. Messenger RNA (mRNA) levels coding for five myelin proteins were assayed in the distal segment of the crush-transected nerve after 9 days and were compared to corresponding levels in the distal segments of sciatic nerves at 21 days post-crush and 21 days post-transection using Northern blot and slot-blot analysis. Levels of mRNAs found in the distal segment of the transected and crush-transected nerve suggested that Schwann cells in the regenerating nerve and in the mature adult nerve are equally responsive to axonal influences. The crush-transected model allowed the genes that were studied to be classified according to their response to Schwann cell-axonal contact. The levels of mRNAs were 1) down-regulated to basal levels (P0 and MBP mRNAs), 2) down-regulated to undetectable levels (myelin-associated glycoprotein mRNAs), 3) upregulated (mRNAs encoding 2'3'-cyclic nucleotide phosphodiesterase and beta-actin), or 4) not stringently controlled by the removal of Schwann cell-axonal contact (proteolipid protein mRNAs). This novel experimental model has thus provided evidence that the expression of some of the important myelin genes during peripheral nerve regeneration is dependent on continuous signals from the ingrowing axons. 相似文献
5.
F. Adrian M. Rü bsam K. -P. Dinse D. Fuchs H. Rietschel R. H. Michel M. Benz M. M. Kappes 《Fullerenes, Nanotubes and Carbon Nanostructures》1996,4(4):655-675
From the analysis of the temperature dependence of the EPR line width, an estimate of the anisotropic parts of the dipolar and quadrupolar hyperfine couplings of lanthanum was derived. Furthermore, the rotational correlation time of the nearly spherical molecule was found to deviate considerably from that of the solvent molecules. 相似文献
6.
Suppression of carbon monoxide formation in oxide-coated TZM molybdenum X-ray rotating anodes 总被引:1,自引:0,他引:1
M. G. Benz M. R. Jackson K. A. Lou R. J. Zabala T. C. Tiearney Jr 《International Journal of Refractory Metals and Hard Materials》1995,13(6):327-336
Higher performance designs for rotating anode X-ray tubes have increased the average rotating anode temperature from below 1100 °C to well above 1300 °C. This temperature increase has accelerated the formation of carbon monoxide by reaction of carbon from the alloy substrate with oxygen from the emissive coating. The dominant carbon source is thought to be Mo2C grain boundary precipitates in the TZM molybdenum alloy substrate. The dominant oxygen source is thought to be TiO in the emissive coating. Placement of a monocarbide-forming reactive layer between the alloy substrate and the emissive coating has been demonstrated to lower the thermodynamic activity of the carbon source and dramatically reduce the rate of formation of carbon monoxide. 相似文献
7.
Chemiresistive polymer composite ribbons that function as chemical detectors were produced from solution-cast films of polymers and carbon composites. An array with multiple polymer sensor threads was exposed to dimethyl methyl phosphonate, a nerve agent simulant, and different interferents in the vapor phase. Principal component analysis was used to differentiate between the analytes. The response of the ribbon sensors as a function of the carbon composite and the host polymer source was investigated. The freestanding threads/sensors were mounted into a cell perpendicular to the gas flow to provide little pressure drop and were imbedded into fabrics to provide an example of a small, low-cost, wearable chemical sensor. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012 相似文献
8.
The surface growth kinetics of CdTe and HgTe have been investigated during molecular and metalorganic molecular beam epitaxy.
The surface growth kinetics was studied through in-situ measurements of the growth rate as a function of flux ratio and substrate
temperature on the (001), (111)B, and (211)B CdTe surface orientations. For the (001) and (111)B CdTe growth kinetics, the
existence of low binding energy surface precursor sites was proposed for both molecular and atomic growth species before lattice
incorporation. Intensity oscillations were observed during HgTe growth on misoriented (111)B surfaces and during CdTe growth
on the (211)B orientation. The (211)B surface reconstructions displayed both vicinal and singular surface characteristics,
depending on the growth flux ratio. 相似文献
9.
A family of sintered Co-rare earth alloys utilizing Sm, Pr, La, or Ce mischmetal has been developed. Each alloy is designed to optimize one of the following characteristics: 1) Magnets which exhibit magnetic energy products in excess of 20 MG . Oe; 2) Magnets which exhibit reversible demagnetization behavior in adverse fields up to 14 000 Oe; 3) Magnets using inexpensive rare earth metals to maximize the magnetic energy per unit cost. These alloys should extend the range of use of Co-rare earth magnets for microwave devices. 相似文献
10.
Unit-cell dimensions of U-C-N alloys were measured at temperatures of 760 to 2250 °C with an X-ray diffractometer. Lattice dimensions of the face-centered cubic phase in both the UC-UC2 and UC-UN solutions are found to be linear functions of the UC mole fraction. In carbon saturated body-centered tetragonal α-UC2 between 1000 and 1400 °C, a small but distinct decrease in rate of volume change (slope) occurs with increasing temperature. The data suggest that the C/U ratio in α-UC2 at 1000 °C and below is 1.97± 0.03, a value in excess of that prevailing in carbon-saturated α-UC2 at higher temperatures. 相似文献