Neuromuscular activation patterns during treadmill walking after space flight

Astronauts adopt a variety of neuromuscular control strategies during space flight that are appropriate for locomoting in that unique environment, but are less than optimal upon return to Earth. We report here the first systematic investigation of potential adaptations in neuromuscular activity patterns associated with postflight locomotion. Astronaut-subjects were tasked with walking on a treadmill at 6.4 km/h while fixating a visual target 30 cm away from their eyes after space flights of 8-15 days. Surface electromyography was collected from selected lower limb muscles and normalized with regard to mean amplitude and temporal relation to heel strike. In general, high correlations (more than 0.80) were found between preflight and postflight activation waveforms for each muscle and each subject: however relative activation amplitude around heel strike and toe off was changed as a result of flight. The level of muscle cocontraction and activation variability, and the relationship between the phasic characteristics of the ankle musculature in preparation for toe off also were altered by space flight. Subjects also reported oscillopsia during treadmill walking after flight. These findings indicate that, after space flight, the sensory-motor system can generate neuromuscular-activation strategies that permit treadmill walking, but subtle changes in lower-limb neuromuscular activation are present that may contribute to increased lower limb kinematic variability and oscillopsia also present during postflight walking.     

Development of clinical indicators for performance measurement and improvement: an HMO/purchaser collaborative effort

This article describes a collaborative effort between a major health care purchaser, three area health maintenance organizations, and a state Medicaid agency to develop clinical indicators as the basis for a comparison database. Some of the difficulties in developing a "common yardstick" of quality and value are candidly discussed.     

Implementation of MB algorithm for nonlinear magnetostatics

The iteration strategy in the MB formulation for nonlinear magnetostatic problems is discussed. The difference between MH and MB iteration is examined, and two methods for implementating the constitutive M(B) relation are presented.     

Summarization of Imaged Documents without OCR

A system is presented for creating a summary indicating the contents of an imaged document. The summary is composed from selected regions extracted from the imaged document. The regions may include sentences, key phrases, headings, and figures. The extracts are identified without the use of optical character recognition. The imaged document is first processed to identify the word-bounding boxes, the reading order of words, and the location of sentence and paragraph boundaries in the text. The word-bounding boxes are grouped into equivalence classes to mimic the terms in a text document. Equivalence classes representing content words are identified, and key phrases are identified from the set of content words. Summary sentences are selected using a statistically based classifier applied to a set of discrete sentence features. Evaluation of sentence selection against a set of abstracts created by a professional abstracting company is given.     

Not just another self-consistent magnetic recording model

A self-consistent magnetic recording modeling method is presented which has proven useful in recording channel design on thin media. Improvements in the magnetic model and in the mathematical treatment stabilize the iterative process and reduce computer storage requirements. Major and minor media loops are fitted to quickly computable bipolynomials. Mathematical improvements include using a strong band diagonal demagnetization matrix, analytic integration with quadratic magnetization fitting, and Newton-Raphson iteration, which gives rapid convergence without underrelaxation. Quantitative predictions of timing errors in 16-bit modified frequency modulation (MFM) data patterns on a 350-bit/mm, 20-track/mm disk memory are presented, as well as playback amplitudes and saturation currents. Predictions can also be compared with experimental read/write data to determine system parameters. Two examples are given: the inference of the head efficiency and of the effective high-frequency medium squareness.     

Spectroscopic studies of the interaction of Ca2+-ATPase-peptides with dodecyl maltoside and its brominated analog

The transmembrane sector of sarcoplasmic reticulum Ca2+-ATPase comprises ten putative transmembrane spans (M1-M10) in current topology models. We report here the structure and properties of three synthetic peptides with a single Trp representing the M6 and M7 regions implicated in Ca2+ binding: peptide M6 (amino acid residues 785-810), peptide M7-L (amino acid residues 808-847) corresponding to loop 6-7 and the majority of span M7, and peptide M7-S (amino acid residues 818-847) which contains a shorter version of loop 6-7 than M7-L. After uptake of the peptides in the hydrophobic environment of dodecyl maltoside micelles, the peptides gain a significant amount of secondary structure, as indicated by their CD spectra. However, the alpha-helical content of M6 is lower than would be expected for a classical transmembrane segment. For M7-L peptide, the L6-7 loop is subject to specific proteolytic cleavage by proteinase K, as in intact Ca2+-ATPase. The formation of the peptide-detergent complexes was followed from the resulting fluorescence intensity changes, either enhancement using n-dodecyl beta-D-maltoside or quenching using the recently introduced brominated analog of n-dodecyl beta-D-maltoside: 7,8-dibromododecyl beta-maltoside [de Foresta, B., Legros, N., Plusquellec, D., le Maire, M. & Champeil, P. (1996) Eur J. Biochem. 241, 343-354]. Our results indicate that M7-L and M7-S are completely taken up by the detergent micelles. In contrast, the M6 peptide, which is highly water soluble, is more loosely associated with the detergent, as is also demonstrated by size-exclusion chromatography. The location of Trp in micelles was evaluated from the quenching observed in mixed micelles of n-dodecyl beta-D-maltoside/7,8-dibromododecyl beta-maltoside, using tryptophan octyl ester and solubilized Ca2+-ATPase as reference compounds. We conclude that W832 in M7 appears to be located near the surface of the micelle, in agreement with its membrane interfacial localization predicted in most Ca2+-ATPase topology models. In contrast, our data suggest that W794 in M6 has a deeper insertion in the micelle although not to the extent predicted by current models of Ca2+-ATPase and the rather short alpha-helix span of M6 may lead to exposure of a significant part of the C-terminal of this peptide to the micelle surface. The results are discussed in relation to the proposed roles of these membrane segments in active transport of Ca2+ ions, in particular, the demonstration that M6 does not behave as a classical transmembrane helix may be correlated with the evidence, from site-directed mutagenesis, that this transmembrane segment should be essential in Ca2+ binding.     

Studies on the structure of a transmembrane region and a cytoplasmic loop of the human red cell anion exchanger (band 3, AE1)

It was previously revealed [Yamaguchi, H. and Uchida, M. (1996) J. Biochem. 120, 474-477] that both intra- and extramolecular high-mannose type Asn-glycans promote the renaturation of reductively denatured bovine pancreatic RNases A and B under oxidation conditions. To characterize the conformational changes of the polypeptides during the renaturation promoted by the intramolecular Asn-glycans, RNase B was compared with its nonglycosylated form, RNase A, as to the features of the regeneration from their reductively denatured species under Cu2+-catalyzed oxidation conditions. The refolding intermediates of RNase B, as compared with those of RNase A, seemed to contain much less impaired disulfide linkages. In agreement with this finding, the proper refolding of RNase B was much faster than that of RNase A, as revealed by the intrinsic fluorescence and 1-anilino-8-naphthalenesulfonate binding of the refolding intermediates. Such a promoting effect was also observed for extramolecular Asn-glycans of the complex as well as of the high-mannose type. In contrast, common mono-, oligo-, and polysaccharides, but not yeast mannan, exhibited much lower stimulatory effects on the oxidative refolding of RNase A.     

Analytic determination of overwrite capability in magnetic recording systems

Analytic design criteria are provided to determine if a digital magnetic recording system can overwrite under worst-case conditions. The worst-case condition is taken to be a bubble of reversed magnetization in an otherwise saturated medium, written by applying current to a stationary recording head. A leading and a trailing transition are formed, creating a large demagnetizing field opposing the head field. Although the leading transition is commonly thought to be unimportant in saturation writing, its demagnetizing field can significantly hamper the writing of the trailing transition. First, self-consistent numerical calculation shows the characteristics of the bubble and its associated fields. Then the bubble is approximated analytically by a biquadratic form, and the demagnetizing field at the bubble center is compared with the field necessary to saturate the medium. A rapid loss of overwrite ability with decreasing gap-length is demonstrated. The importance of including image fields for thin-film heads is discussed. Graphical representations are given for the minimum gap-length necessary for overwrite. A relation between media parameters, bit density, and magnetic energy/bit in commercial disk drives is discussed.     

Spectral response from perpendicular media with gapped head and underlayer

A general spectral formulation is presented, in the spirit of the Karlquist approximation, for the field from a gapped head with a permeable underlayer. For an infinite permeability underlayer, of particular interest in perpendicular recording, the fields and readback response are derived for sine-wave and square-wave magnetization, as well as for an isolated transition. The sine-wave response is given in a simple form, allowing interpretation in terms of a reduced pole density from the medium at the underlayer. The square-wave response is given as a Fourier series of sine-wave amplitudes. Approximate analytical functions are constructed for the response to an isolated transition.