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排序方式: 共有68条查询结果,搜索用时 15 毫秒
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Mumcuoglu EU Hassanpour R Tasel SF Perkins G Martone ME Gurcan MN 《Journal of microscopy》2012,246(3):248-265
Mitochondrial function plays an important role in the regulation of cellular life and death, including disease states. Disturbance in mitochondrial function and distribution can be accompanied by significant morphological alterations. Electron microscopy tomography (EMT) is a powerful technique to study the 3D structure of mitochondria, but the automatic detection and segmentation of mitochondria in EMT volumes has been challenging due to the presence of subcellular structures and imaging artifacts. Therefore, the interpretation, measurement and analysis of mitochondrial distribution and features have been time consuming, and development of specialized software tools is very important for high-throughput analyses needed to expedite the myriad studies on cellular events. Typically, mitochondrial EMT volumes are segmented manually using special software tools. Automatic contour extraction on large images with multiple mitochondria and many other subcellular structures is still an unaddressed problem. The purpose of this work is to develop computer algorithms to detect and segment both fully and partially seen mitochondria on electron microscopy images. The detection method relies on mitochondria's approximately elliptical shape and double membrane boundary. Initial detection results are first refined using active contours. Then, our seed point selection method automatically selects reliable seed points along the contour, and segmentation is finalized by automatically incorporating a live-wire graph search algorithm between these seed points. In our evaluations on four images containing multiple mitochondria, 52 ellipses are detected among which 42 are true and 10 are false detections. After false ellipses are eliminated manually, 14 out of 15 fully seen mitochondria and 4 out of 7 partially seen mitochondria are successfully detected. When compared with the segmentation of a trained reader, 91% Dice similarity coefficient was achieved with an average 4.9 nm boundary error. 相似文献
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Soner Sismanoglu Aliye Tugce Gurcan Zuhal Yildirim-Bilmez Burak Gumustas 《International Journal of Applied Ceramic Technology》2021,18(2):312-318
Information on the mechanical properties and repairability of computer-aided design/computer-aided manufacturing (CAD/CAM) blocks is scarce. Five CAD/CAM blocks with similar indications were evaluated, including four resin nanoceramics, one polymer-infiltrated ceramic network (PICN), and one feldspathic ceramic. CAD/CAM blocks were sectioned into 4 mm × 1.2 mm × 13 mm bars for flexural strength (FS), and 4 mm thick blocks were prepared for elastic modulus (EM), nanohardness (NH), and microshear bond strength (µSBS) testing. FS of the CAD/CAM blocks was determined using a three-point bending test, whereas EM and NH values were measured using a nanoindenter. The reparability of the tested block materials was determined by the µSBS test. One-way ANOVA was conducted for FS, EM, NH, and µSBS followed by Tukey's pairwise comparison (α < 0.05). FS ranged from 115 to 207 MPa, EM from 8.21 to 44 GPa, NH from 0.76 to 7.24, and µSBS from 24.9 to 30.6 MPa. The findings of the present study revealed that resin nanoceramic blocks exhibited higher FS values than PICN and feldspathic ceramic, and they have acceptable mechanical properties for the fabrication of single-unit restorations according to the ISO 6872:2008. Furthermore, all CAD/CAM blocks tested can be successfully repaired regarding their recommended repair protocol. 相似文献
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Biodegradation of pretreated olive mill effluent in mixture with a domestic sewage or compatible wastewater stream
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Differentiation of Mechanically and Chemically Extracted Hazelnut Oils Based on their Sterol and Wax Profiles
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Cansu Ekin Gumus Asli Yorulmaz Aziz Tekin 《Journal of the American Oil Chemists' Society》2016,93(12):1625-1635
The sterol and wax content of solvent extracted (SEHO) and cold pressed hazelnut oils (CPHO) were compared. A total of 48 samples from 19 hazelnut varieties were collected for two successive crop years from four different geographical districts in Turkey. Hazelnuts were processed to oil with a laboratory scale press, than the remaining oil in cake was extracted with n‐hexane. CPHO and SEHO were evaluated for their wax, sterol and squalene contents. Results showed that sterol, squalene and wax contents of all individual cultivars were higher in SEHO than those of CPHO, indicating the higher solubility of these compounds in solvent. Total sterol contents ranged between 1088.56 (Kargalak)—1609.39 mg/kg (Mincane) for CPHO and 1590.86 (Çak?ldak)—2897.26 mg/kg (Mincane) for SEHO. Hazelnut oils were found to be richer of C36‐38 esters than C40‐46 group. Total wax content was between 24.19 (Kargalak)—94.58 mg/kg (Ku?) for CPHO and 81.46 (Kargalak)—160.92 mg/kg (Akçakoca) for SEHO. The squalene amounts of the samples obtained by hexane extraction were between 499.75 (Allahverdi)—885.36 mg/kg (Cavcava), while it varied between 288.55 (Kargalak)—647.68 mg/kg (Mincane) in cold pressed oils. Significant and obvious variations between SEHO and CPHO were verified by principal component and hierarchical cluster analysis. Geographical discrimination was also achieved by discriminant analysis. 相似文献
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This study was carried out in a Turkish university with 216 undergraduate students of computer technology as respondents. The study aimed to develop a scale (UECUBS) to determine the unethical computer use behavior. A factor analysis of the related items revealed that the factors were can be divided under five headings; intellectual property, social impact, safety and quality, net integrity and information integrity. 相似文献
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This article examines the optimization of throughput in a wireless system. A mathematical model is outlined for a cellular wideband CDMA system. In practical cases, where the maximum transmission power is constrained, power control may not be possible. Self-interference limits the total deliverable rate. It is shown that by optimally allocating bit-rate using a dynamic programming algorithm, a higher bit-rate can be delivered within the same power constraint. Simulation results show on average a 20% increase in total delivered rate can be achieved. 相似文献
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Hadiseh Taheri Mehmet Altay Unal Melike Sevim Cansu Gurcan Okan Ekim Ahmet Ceylan Zois Syrgiannis Konstantinos C. Christoforidis Susanna Bosi Ozge Ozgen Manuel Jos Gmez Mine Turktas Erken igdem Soydal Zafer Erolu Ceylan Verda Bitirim Umut Cagin Fikret Ar Asuman Ozen Ozlem Kuuk Lucia Gemma Delogu Maurizio Prato
nder Metin Aelya Yilmazer 《Small (Weinheim an der Bergstrasse, Germany)》2020,16(10)