Combating Biofilm Associated Infection In Vivo: Integration of Quorum Sensing Inhibition and Photodynamic Treatment based on Multidrug Delivered Hollow Carbon Nitride Sphere

Recently, quorum sensing (QS) inhibitors (QSIs) have been combined with antibiotics to enhance antibiofilm efficacy in vitro and in vivo. However, targeting QS signals alone is not enough to prevent bacterial infections. Drug resistance and recurrence of biofilms makes it difficult to eradicate. Herein, photodynamic therapy (PDT) is selected to unite QSIs and antibiotics. A synergistically antibiofilm system, which combines QSIs, antibiotics, and PDT based on hollow carbon nitride spheres (HCNSs) is envisaged. First, HCNS provides the multidrug delivering ability, enabling QSIs and antibiotics to be released in sequence. Subsequently, multistage releases sensitize bacteria effectively, potentiating the chemotherapeutic effects of the antibiotics. Finally, the integration of QSIs and PDT not only minimizes the possibility of drug resistance, but also overcomes the problem of limited mass and extension of PDT. Even after 48 h of incubation, the bacterial biofilm is obviously inhibited. And its biofilm disperse efficiency exceeds 48% (compared with QSI‐potentiated chemotherapy group) and 40% (compared with PDT group). Besides, the inhibition of the QS system influences phenotypes related to virulence factor production and surface hydrophobicity, which weaken biofilm invasion and formation. Eventually, this system is applied to disperse bacterial biofilm in vivo. Overall, PDT and QS modulation are devoted to eradicate drug resistance and recurrence of the biofilm.     

基于Arduino的目标跟踪系统设计

利用Arduino设计并实现了一种目标跟踪系统。该系统包括图像采集处理、基于Arduino开发的下位机软件和舵机云台3个模块。相机实时接收图像,对捕获到的图像进行预处理,调用OpenCV中的函数对图像进行霍夫圆检测。计算机通过串口与Arduino开发板连接,将图形位置信息数据实时发送。通过Arduino控制舵机的转动角度,精确调整相机的方位、俯仰姿态,完成实时的目标追踪。实验结果表明,在使用Python开发的计算机软件中,通过动态目标视觉检测以及PID控制的角度输出,舵机云台实现了很好的控制效果,使二维转台实现圆形识别跟踪。     

HIGH TEMPERATURE LOW CYCLIC FATIGUE BEHAVIOUR OF DIRECTIONALLY SOLIDIFIED Ni-BASE ALLOY DK3

研究了镍基定向凝固高温合金DK3和普通铸造合金K3在760℃应变控制和应力控制下的高温低周疲劳行为。结果表明:这两种合金的高温低周疲劳行为可以用常规的应力疲劳曲线来表征。DK3合金不存在“过渡疲劳寿命”N_T,K3合金则很低。在各种不同应变或应力水平下,DK3合金的低周疲劳寿命高于K3合金,当应力水平为785至980MPa时,其寿命约差1倍,当应力水平为490至685MPa时,则差2倍多。     

OCDMA系统中变重光正交码的构造及误码率分析

变重光正交码是基于不同用户带有不同的QoS(Quality of Service)需求而提出的,由于它的码重不是固定值,使得其码字的容量较之常重光正交码有所增加.给出了变重光正交码的定义及其码字容量界,介绍了构造变重光正交码的一种方法,给出一些码字的构造结果,并对码重数不同的码字进行多址干扰误码率的仿真比较和理论分析.结果表明:在相同的码重数下,码重越大的误码率越小;而当码重数不同时,在相同的码重下,码重数大的误码率小.     

基于BIM5D技术施工阶段成本控制模型构建

基于先进的BIM5D技术,结合挣值法,深刻挖掘BIM5D技术在成本控制中的潜在优势,系统性地分析成本控制模型的系统实施流程,并通过北京某信息大厦成本控制过程论证了基于BIM5D技术施工阶段成本控制模型构建的可行性。     

EFFECT OF VOLUME FRACTION AND SIZE OF FINE γ' ON CREEP STRENGTH OF A DS NICKEL-BASE SUPERALLOY

本文研究了定向凝固K3镍基高温合金的蠕变强度与细小γ′粒子的数置和尺寸的关系。实验结果证明,随着固溶温度升高,铸态粗大γ′逐步溶解并在随后冷却过程重新析出均匀细小正方形的γ′粒子。细小γ′体积分数(v_f)和尺寸(α)都随固溶温度的升高而增大,当固溶温度从1100℃升至1230℃,v_f从0.25增至0.63,α从0.10μm增至0.32μm。随着固溶温度的升高,第二阶段蠕变速率降低,持久寿命延长,大幅度提高合金的蠕变性能。适当的高温固溶加时效处理(如1210—1230℃,4h+900℃,32h)可提高定向凝固合金的中温(760℃,73.8kgf/mm~2)持久寿命10倍左右。 合金的中温蠕变性能取决于细小γ′的体积分数(v_f),尺寸(α)及其间距(λ),在固定温度和应力下,第二阶段蠕变速率((?))与它们之间符合以下关系。 (?)∝ λ~2/α或(?)∝ α/v_f~(2/3)(1-v_f~(1/3))~2 用透射电镜观察了合金三个蠕变阶段位错亚结构的变化,据此提出蠕变的位错模型和合金的强化机制,并导出第二阶段蠕变速率与γ′体积分数、尺寸和间距之间的关系式,与实验结果完全符合。     

Targeting orthotopic gliomas with renal-clearable luminescent gold nanoparticles

A major clinical translational challenge in nanomedicine is the potential of toxicity associated with the uptake and long-term retention of non-degradable nanoparticles (NPs) in major organs.The development of inorganic NPs that undergo renal clearance could potentially resolve this significant biosafety concern.However,it remains unclear whether inorganic NPs that can be excreted by the kidneys remain capable of targeting tumors with poor permeability.Glioblastoma multiforme,the most malignant orthotopic brain tumor,presents a unique challenge for NP delivery because of the blood-brain barrier and robust blood-tumor barrier of reactive microglia and macroglia in the tumor microenvironment.Herein,we used an orthotopic murine glioma model to investigate the passive targeting of glutathione-coated gold nanoparticles (AuNPs) of 3 nm in diameter that undergo renal clearance and 18-nm AuNPs that fail to undergo renal clearance.Remarkably, we report that 3-nm AuNPs were able to target intracranial tumor tissues with higher efficiency (2.3x relative to surrounding non-tumor normal brain tissues) and greater specificity (3.0x)than did the larger AuNPs.Pharmacokinetics studies suggested that the higher glioma targeting ability of the 3-nm AuNPs may be attributed to the longer retention time in circulation.The total accumulation of the 3-nm AuNPs in major organs was significantly less (8.4x) than that of the 18-nm AuNPs.Microscopic imaging of blood vessels and renal-clearable AuNPs showed extravasation of NPs from the leaky blood-tumor barrier into the tumor interstitium.Taken together,our results suggest that the 3-nm AuNPs,characterized by enhanced permeability and retention,are able to target brain tumors and undergo renal clearance.     

磷含量对真空蒸镀磷掺杂多晶硅薄膜的影响

采用真空蒸镀的方法制备磷掺杂多晶硅薄膜,研究了磷含量对多晶硅薄膜的表面形貌、组织结构、晶粒尺寸及晶化率的影响。结果表明:随着磷掺杂分数的增加,多晶硅薄膜的晶粒尺寸和晶化率表现出先增加后降低的趋势,当磷掺杂分数为1%时,薄膜晶粒尺寸达到最大值,为0.55μm,晶化率为96.82%,且晶粒的均匀性最佳。     

一种适用于中性点多扁钢入地的变压器直流偏磁电流测量方法

变压器中性点直流偏磁电流的准确测量是开展电网直流偏磁监测、防御和治理的重要理论基础.针对变压器中性点多扁钢入地条件下,传统的扁钢采样电阻直流偏磁电流测量方法存在的问题,在不增加电流源的前提下,采用多扁钢等电阻同时采样的测量回路,提出了一种可准确测量多根扁钢流经直流偏磁电流的方法,分析了该方法中多扁钢采样阻值偏差对测量准确度的影响,并通过真形扁钢试验,验证了该方法的可行性.该方法可用于直流偏磁电流的在线监测和带电检测,具有较好的实用推广价值.     

A new approach to pointer analysis for assignments

Pointer analysis is a technique to identify at copile-time the potential values of the pointer expressions in a program,which promises significant benefits for optimzing and parallelizing complilers.In this paper,a new approach to pointer analysis for assignments is presented.In this approach,assignments are classified into three categories:pointer assignments,structure(union)assignents and normal assignments which don‘t affect the point-to information.Pointer analyses for these three kinds of assignments respectively make up the integrated algorithm.When analyzing a pointer assigemtn.a new method called expression expansion is used to calculate both the left targets and the right targets.The integration of recursive data structure analysis into pointer analysis is a significant originality of this paper,which uniforms the pointer analysis for heap variables and the pointer analysis for stack variables.This algorithm is implemented in Agassiz,an analyzing tool for C programs developed by Institute of Parallel Processing,Fudan University,Its accuracy and effectiveness are illustrated by experimental data.