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Veronica M.T. Lattanzio Biancamaria Ciasca Valeria Terzi Roberta Ghizzoni Susan P. McCormick Michelangelo Pascale 《Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment》2015,32(10):1647-1655
This paper reports a new method for the determination of T-2 and HT-2 toxins and their glucosylated derivatives in cereals, and some survey data aimed at obtaining more comprehensive information on the co-occurrence of T-2 and HT-2 toxins and their glucosylated derivatives in naturally contaminated cereal samples. For these purposes, barley samples originating from a Northern Italian area were analysed by LC-HRMS for the presence of T-2, HT-2 and relevant glucosyl derivatives. Quantitative analysis of T-2 and HT-2 glucosides was performed for the first time using a recently made available standard of T-2 glucoside. The glucosyl derivative of HT-2 was detected at levels up to 163 µg kg–1 in 17 of the 18 analysed unprocessed barley grains, whereas the monoglucosyl derivative of T-2 toxin was detected in only a few samples and at low µg kg–1 levels. The ratio between glucosylated toxins (sum of T-2 and HT-2 glucosides) and native toxins (sum of T-2 and HT-2) ranged from 2% to 283%. Moreover, taking advantage of the possibility of retrospective analysis of full-scan HRMS chromatograms, samples were also screened for the presence of other type-A trichothecenes, namely neosolaniol, diacetoxyscirpenol and their monoglucosyl derivatives, which were detected at trace levels. A subset of nine different samples was subjected to micro-maltation in order to carry out a preliminary investigation on the fate of T-2, HT-2 and relevant glucosides along the malting process. Mycotoxin reduction from cleaned barley to malt was observed at rates ranging from 4% to 87%. 相似文献
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Umberto Basile Cecilia Napodano Francesca Gulli Krizia Pocino Riccardo Di Santo Laura Todi Valerio Basile Carlo Provenzano Gabriele Ciasca Mariapaola Marino 《International journal of molecular sciences》2021,22(17)
Myasthenia gravis with antibodies (Abs) against the muscle-specific tyrosine kinase (MuSK) is a rare autoimmune disorder (AD) of the neuromuscular junction (NMJ) and represents a prototype of AD with proven IgG4-mediated pathogenicity. Thanks to the mechanism of Fab-arm exchange (FAE) occurring in vivo, resulting MuSK IgG4 k/λ Abs increase their interference on NMJ and pathogenicity. The characterization of hybrid MuSK IgG4 as a biomarker for MG management is poorly investigated. Here, we evaluated total IgG4, hybrid IgG4 k/λ, and the hybrid/total ratio in 14 MuSK-MG sera in comparison with 24 from MG with Abs against acetylcholine receptor (AChR) that represents the not IgG4-mediated MG form. In both subtypes of MG, we found that the hybrid/total ratio reflects distribution reported in normal individuals; instead, when we correlated the hybrid/total ratio with specific immune-reactivity we found a positive correlation only with anti-MuSK titer, with a progressive increase of hybrid/total mean values with increasing disease severity, indirectly confirming that most part of hybrid IgG4 molecules are engaged in the anti-MuSK pathogenetic immune-reactivity. Further analysis is necessary to strengthen the significance of this less unknown biomarker, but we retain it is full of a diagnostic-prognostic powerful potential for the management of MuSK-MG. 相似文献
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Ciasca G. Papi M. Chiarpotto M. De Ninno A. Giovine E. Campi G. Gerardino A. De Spirito M. Businaro L. 《纳微快报(英文)》2014,6(3):280-286
Nano-Micro Letters - In this paper we provide evidence that the Cassie-to-Wenzel transition, despite its detrimental effects on the wetting properties of superhydrophobic surfaces, can be exploited... 相似文献
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Alessandro Ricci Nicola Poccia Gabriele Ciasca Michela Fratini Antonio Bianconi 《Journal of Superconductivity and Novel Magnetism》2009,22(6):589-593
The 3D phase diagram of iron pnictides where the critical temperature depends on charge density and microstrain in the active
FeAs layers is proposed. The iron pnictides superconductors are shown to be a practical realization of a heterostructure at
the atomic limit made of a superlattice of FeAs layers intercalated by spacer layers. We have focussed our interest on the
A1−x
B
x
Fe2As2 (122) families and we show that FeAs layers have a tensile microstrain due to the misfit strain between the active layers
and the spacers. We have identified the critical range of doping and microstrain where the critical temperature gets amplified
to its maximum value. 相似文献
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Giordano Perini Enrico Rosa Ginevra Friggeri Lorena Di Pietro Marta Barba Ornella Parolini Gabriele Ciasca Chiara Moriconi Massimiliano Papi Marco De Spirito Valentina Palmieri 《International journal of molecular sciences》2022,23(6)
Cancer spheroids are in vitro 3D models that became crucial in nanomaterials science thanks to the possibility of performing high throughput screening of nanoparticles and combined nanoparticle-drug therapies on in vitro models. However, most of the current spheroid analysis methods involve manual steps. This is a time-consuming process and is extremely liable to the variability of individual operators. For this reason, rapid, user-friendly, ready-to-use, high-throughput image analysis software is necessary. In this work, we report the INSIDIA 2.0 macro, which offers researchers high-throughput and high content quantitative analysis of in vitro 3D cancer cell spheroids and allows advanced parametrization of the expanding and invading cancer cellular mass. INSIDIA has been implemented to provide in-depth morphologic analysis and has been used for the analysis of the effect of graphene quantum dots photothermal therapy on glioblastoma (U87) and pancreatic cancer (PANC-1) spheroids. Thanks to INSIDIA 2.0 analysis, two types of effects have been observed: In U87 spheroids, death is accompanied by a decrease in area of the entire spheroid, with a decrease in entropy due to the generation of a high uniform density spheroid core. On the other hand, PANC-1 spheroids’ death caused by nanoparticle photothermal disruption is accompanied with an overall increase in area and entropy due to the progressive loss of integrity and increase in variability of spheroid texture. We have summarized these effects in a quantitative parameter of spheroid disruption demonstrating that INSIDIA 2.0 multiparametric analysis can be used to quantify cell death in a non-invasive, fast, and high-throughput fashion. 相似文献
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Ciasca Gabriele Papi Massimiliano Palmieri Valentina Chiarpotto Michela Di Claudio Simone De Ninno Adele Giovine Ennio Campi Gaetano Gerardino Annamaria Businaro Luca De Spirito Marco 《纳微快报(英文)》2015,7(2):146-151
Nano-Micro Letters - The use of superhydrophobic surfaces (SHSs) is now emerging as an attractive platform for the realization of one-dimensional (1D) nanostructures with potential applications in... 相似文献
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