Volumetric imaging with homogenised excitation and static field at 9.4 T

Objectives

To overcome the challenges of B₀ and RF excitation inhomogeneity at ultra-high field MRI, a workflow for volumetric B₀ and flip-angle homogenisation was implemented on a human 9.4 T scanner.

Materials and methods

Imaging was performed with a 9.4 T human MR scanner (Siemens Medical Solutions, Erlangen, Germany) using a 16-channel parallel transmission system. B₀- and B₁-mapping were done using a dual-echo GRE and transmit phase-encoded DREAM, respectively. B₀ shims and a small-tip-angle-approximation kT-points pulse were calculated with an off-line routine and applied to acquire T₁- and T ₂ ^* -weighted images with MPRAGE and 3D EPI, respectively.

Results

Over six in vivo acquisitions, the B₀-distribution in a region-of-interest defined by a brain mask was reduced down to a full-width-half-maximum of 0.10 ± 0.01 ppm (39 ± 2 Hz). Utilising the kT-points pulses, the normalised RMSE of the excitation was decreased from CP-mode’s 30.5 ± 0.9 to 9.2 ± 0.7 % with all B ₁ ⁺  voids eliminated. The SNR inhomogeneities and contrast variations in the T₁- and T ₂ ^* -weighted volumetric images were greatly reduced which led to successful tissue segmentation of the T₁-weighted image.

Conclusion

A 15-minute B₀- and flip-angle homogenisation workflow, including the B₀- and B₁-map acquisitions, was successfully implemented and enabled us to reduce intensity and contrast variations as well as echo-planar image distortions in 9.4 T images.

     

Magnetic resonance imaging at 9.4 T: the Maastricht journey

The 9.4 T scanner in Maastricht is a whole-body magnet with head gradients and parallel RF transmit capability. At the time of the design, it was conceptualized to be one of the best fMRI scanners in the world, but it has also been used for anatomical and diffusion imaging. 9.4 T offers increases in sensitivity and contrast, but the technical ultra-high field (UHF) challenges, such as field inhomogeneities and constraints set by RF power deposition, are exacerbated compared to 7 T. This article reviews some of the 9.4 T work done in Maastricht. Functional imaging experiments included blood oxygenation level-dependent (BOLD) and blood-volume weighted (VASO) fMRI using different readouts. BOLD benefits from shorter T₂* at 9.4 T while VASO from longer T₁. We show examples of both ex vivo and in vivo anatomical imaging. For many applications, pTx and optimized coils are essential to harness the full potential of 9.4 T. Our experience shows that, while considerable effort was required compared to our 7 T scanner, we could obtain high-quality anatomical and functional data, which illustrates the potential of MR acquisitions at even higher field strengths. The practical challenges of working with a relatively unique system are also discussed.

     

Challenges facing the European power transmission tariffs: The case of inter-TSO compensation

This article draws attention to problems important for all EU power consumers—the unfairness in individual payments for power transmission and in the cross-border subsidy element in the mechanism of Inter-Transmission System Operators (TSO) Compensation (ITC). A brief review of power transmission tariffs brings out the structure of the problems. A short retrospection explains their growth. The essence of the ITC mechanism is explained and existing shortcomings are illustrated. The deficiencies of existing regulations for transmission pricing are analyzed. In the light of this analysis, the ITC problem is reconsidered and defined more precisely. The basic prerequisites to an ITC reformulation process are presented. The main principles of a new simple, transparent and equitable approach are suggested, in accordance with the contemporary legal positions and functions of the TSOs.     

Analytically determining of the absolute inaccuracy (error) of indirectly measurable variable and dimensionless scale characterising the quality of the experiment

In the following paper we present an easily applicable new method for the analytical representation of the maximum absolute inaccuracy (error) of an indirectly measurable variable f = f(x₁, x₂, ..., x_n) as a function of the maximum absolute inaccuracies (errors) of the directly measurable variables x₁, x₂, ..., x_n. Our new approach is more adequate for the objective reality. The gist of it is that in order to find the analytical form of the maximum absolute inaccuracy of the variable f we take for being fixed variables the statistical mean values of the modules of the moment velocities of alteration of f in respect of the variables x₁, x₂, ..., x_n and the numerical value of the maximum absolute inaccuracy of the variable f is found using the statistical mean values of the absolute values of the absolute inaccuracies . Having this in mind we develop the theory of errors, which we will call with what we feel is a more precise term — theory of inaccuracies. We introduce some new terms — space of the absolute inaccuracy and stochastic plane of the absolute inaccuracy of f. We also define a sample plane of the ideal absolutely accurate experiment and using it we define a universal numerical characteristic — a dimensionless scale for evaluation of the quality (accuracy) of the experiment.     

Effect of Quercetin and Fingolimod,Alone or in Combination,on the Sphingolipid Metabolism in HepG2 Cells

Combinations of anti-cancer drugs can overcome resistance to therapy and provide new more effective treatments. In this work we have analyzed the effect of the polyphenol quercetin and the anti-cancer sphingosine analog fingolimod on the sphingolipid metabolism in HepG2 cells, since sphingolipids are recognized as mediators of cell proliferation and apoptosis in cancer cells. Treatment of hepatocellular carcinoma HepG2 cells with quercetin and fingolimod, alone or in combination, induced different degrees of sphingomyelin (SM) reduction and a corresponding activation of neutral sphingomyelinase (nSMase). Western blot analysis showed that only treatments containing quercetin induced up-regulation of nSMase expression. The same treatment caused elevation of ceramide (CER) levels, whereas the observed alterations in sphingosine (SPH) content were not statistically significant. The two tested drugs induced a reduction of the pro-proliferative sphingolipid, sphingosine 1 phosphate (S1P), in the following order: quercetin, fingolimod, quercetin + fingolimod. The activity of the enzyme responsible for CER hydrolysis, alkaline ceramidase (ALCER) was down-regulated only in the incubations involving quercetin and fingolimod did not affect this activity. The enzyme, maintaining the balance between apoptosis and proliferation, sphingosine kinase 1 (SK1), was down-regulated by incubations in the following order: quercetin, fingolimod, quercetin + fingolimod. Western blot analysis showed down-regulation in SK1 expression upon quercetin but not upon fingolimod treatment. Studies on the effect of quercetin and fingolimod on the two proteins associated with apoptotic events, AKT and Bcl-2, showed that only quercetin, alone or in combination, down-regulated the activity of the two proteins. The reported observations provide information which can be useful in the search of novel anti-tumor approaches, aiming at optimization of the therapeutic effect and maximal preservation of healthy tissues.     

Effects of air susceptibility on proton resonance frequency MR thermometry

Object  

The temperature dependence of the proton resonance frequency (PRF) is often used in MR thermometry. However, this method is prone to even very small changes in local magnetic field strength. Here, we report on the effects of susceptibility changes of surrounding air on the magnetic field inside an object and their inferred effect on the measured MR temperature.     

Towards a Multinational Car License Plate Recognition System

A full-fledged image-based car license plate recognition (CLPR) system is described in the paper. CLPR provides an inexpensive automatic solution for remote vehicle identification. Gray-level input images are assumed. The localization stage of the CLPR yields a plate clip followed by character segmentation and recognition. The recognition scheme combines adaptive iterative thresholding with a template-matching algorithm. The method is invariant to illumination and is robust to character size and thickness, skew and small character breaks. Promising results have been obtained in the experiments with Israeli and Bulgarian license plates including images of poor quality. Also, the possibility of using an “off-the-shelf” OCR has been explored.