A protein which masks galactose receptor mediated phage susceptibility in Lactococcus lactis subsp. lactis MPL56

A 28.5-kb plasmid, isolated from Lactococcus lactis subsp. lactis MPL56, causes complete inhibition of four lactococcal phages. Cell wall characteristics of wild-type strain MPL56 were compared with its 28.5 kb plasmid-cured, phage-sensitive derivative MPL56-22. After proteolytic enzyme treatments, adsorption of phages occurred at high levels, an example is 94.6–98.5% in MPL56 cells. Analysis of cell wall extracts of MPL56-22 by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) indicated that the only difference between strains was the 55.4 kDa band in protein patterns of MPL56. Adsorption of the four phages was completely inhibited when MPL56-22 cells were subjected to SDS, Triton-X-100, HCl and NaOH treatments. Lectins that were specific for glucose/mannose and N-acetylglucosamine did not prevent adsorption of phages in cell wall extracts of MPL 56-22. However a lectin specific for galactose (MCA; Momordica charantia ) completely inhibited adsorption of these phages in cell wall extracts of MPL56-22. HPLC patterns of cell wall carbohydrates of MPL56-22 and its HCl treated preparations showed that the most prevalent difference was the galactose on untreated MPL56-22 cell wall chromatograms.     

Relation between abrasion resistance and flexural strength of high volume fly ash concrete

In this paper, abrasion of high volume fly ash (HVFA) concretes made with 50% and 70% of cement replacement with fly ash was assessed in terms of its relation to flexural tensile strength. Comparisons were made between normal Portland cement (NPC) concrete and fly ash concrete. Comparisons were also made between fly ash concretes. Investigation results have shown that the abrasion resistance increased as flexural tensile strength increased. Analysis of the results showed that, for concrete with tensile strength of greater than 4–5 MPa, the abrasion resistance of HVFA concrete with 70% replacement with cement was found to be higher than that of counterpart control NPC concrete and concrete made with 50% fly ash. The comparison between the relation of abrasion to compressive strength and abrasion to flexural tensile strength made in terms of R² of the linear regression showed that a stronger relation existed between abrasion and flexural tensile strength than that of abrasion to compressive strength of the concrete studied.

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Résumé L'étude a pour but d'estimer la relation entre la résistance à l'usure avec la résistance à la traction en flexion des bétons contenant de 50% et 70% de cendres volantes. On a comparé le béton pur au béton contenant des cendres volantes. Des comparaisons ont été faites également entre les différents bétons contenant des cendres volantes. Les resultats de la recherche ont montré que la résistance à l'usure augmente à mesure que la résistance à la traction en flexion de 4∼5 MPa ont une résistance à l'usure plus grande s'ils contienneint 70% de cendres volantes que s'ils étaient purs ou contenaient 50% de cendres volantes. La comparaison entre les relation de la résistance à l'usure en compression et de la résistance à l'usure en traction a été établie en termes de R² de la régression linéaire. On a prouvé qu'une relation plus forte a été obtenue entre la résistance à l'usure et la résistance à la traction en flexion par rapport à la résistance à l'usure en compression du béton étudié.

     

A method for preparing collagen graft materials

The aim of this research was to develop a method of local production of collagen graft materials which are presently imported. The following methods were used to produce collagen membrane and sponge from human placentas and rat tail tendons. Collagen type I was isolated from human placenta and rat tail tendon by acetic acid extraction and characterised by SDS-PAGE. The collagen sponge was prepared by dissolving the collagen in HCl. The resulting dispersion was poured into a glass container, freeze-dried and then cross-linked by immersion in glutaraldehyde solution. It was then washed with distilled water and freeze-dried again. The collagen membrane was also similarly prepared by dispersing lyophilized collagen in HCl but then mixed with glutaraldehyde, exposed to U.V. light and later air dried.     

Chebyshev wavelet collocation method for magnetohydrodynamic flow equations

This study proposes Chebyshev wavelet collocation method for partial differential equation and applies to solve magnetohydrodynamic (MHD) flow equations in a rectangular duct in the presence of transverse external oblique magnetic field. Approximate solutions of velocity and induced magnetic field are obtained for steady‐state, fully developed, incompressible flow for a conducting fluid inside the duct. Numerical results of the MHD flow problem show that the accuracy of proposed method is quite good even in the case of a small number of grid points. The results for velocity and induced magnetic field are visualized in terms of graphics for values of Hartmann number H_a ≤ 1000.

     

An artificial neural network model for the effects of chicken manure on ground water

In the areas where broiler industry is located, poultry manure from chicken farms could be a major source of ground water pollution, and this may have extensive effects particularly when the farms use nearby ground water as their fresh water supply. Therefore the prediction the extent of this pollution, either from rigorous mathematical diffusion modeling or from the perspective of experimental data evaluation bears importance. In this work, we have investigated modeling of the effects of chicken manure on ground water by artificial neural networks. An ANN model was developed to predict the total coliform in the ground water well in poultry farms. The back-propagation algorithm was employed for training and testing the network, and the Levenberg-Marquardt algorithm was utilized for optimization. The MATLAB 7.0 environment with Neural Network Toolbox was used for coding. Given the associated input parameters such as the number of chickens, type of manure pool management and depth of well, the model estimates the possible amount of total coliform in the wells to a satisfactory degree. Therefore it is expected to be of help in future for estimating the ground water pollution resulting from chicken farms.     

Effects of surface roughness and the chemical structure of materials of construction on wall slip behavior of linear low density polyethylene in capillary flow

The presence of wall slip during the flow of polymeric melts has significant ramifications on the melts' processability. In this study, the effects of materials of construction and surface roughness on the wall slip behavior of a linear low density polyethylene were investigated, using capillary flow. Capillaries, constructed from copper, stainless steel, aluminum, and glass, were used. The inner surface roughness of the capillaries were characterized by the employment of a profilometer and scanning electron microscopy. The roughness profiles of copper capillaries were also altered by the employment of chemical etching. Using Mooney's analysis, the wall slip velocity values were determined to be in the range of 0.09 to 1.34 mm/s. The wall slip velocity values were the highest for stainless steel and were negligible for aluminum. The relative work of adhesion values of polyethylene were the smallest for stainless steel and copper and the highest for glass. Overall, the wall slip velocity values increased with decreasing surface roughness of the capillaries and with decreasing work of adhesion. © 1993 John Wiley & Sons, Inc.     

(BaTiO3)1-x + (Co0.5Ni0.5Nb0.06Fe1.94O4)x nanocomposites: Structure,morphology, magnetic and dielectric properties

Two phase-based nanocomposites consisting of dielectric barium titanate (BaTiO₃ or BTO) and magnetic spinel ferrite Co_0.5Ni_0.5Nb_0.06Fe_1.94O₄ (CNNFO) have been synthesized through solid state route. Series of (BaTiO₃)_1-x + (Co_0.5Ni_0.5Nb_0.06Fe_1.94O₄)_x nanocomposites with x content of 0.00, 0.25, 0.50, 0.75, and 1.00 were considered. The structure has been examined via X-rays diffraction (XRD) and indicated the occurrence of both perovskite BTO and spinel CNNFO phases in various nanocomposites. A phase transition from tetragonal BTO structure to cubic structure occurs with inclusion of CNNFO phase. The average crystallites size of BTO phase decreases, whereas that for the CNNFO phase increases with increasing x in various nanocomposites. The morphological observations revealed that the porosity is highly reduced, and the connectivity between grains is enhanced with increasing x content. The optical properties have been investigated by UV−vis diffuse reflectance spectroscopy. The deduced band gap energy (E_g) value is found to reduce with increasing the content of spinel ferrite phase. The magnetic as well as the dielectric properties were also investigated. The analysis showed that CNNFO ferrite phase greatly affects the magnetic properties and dielectric response of BTO material. The obtained findings can be useful to enhance the performances of magneto-dielectric composite-based systems.     

Determination of the optimum conditions for ion nitriding of AISI 5140 steel

AISI 5140 low alloy steel was ion nitrided under different process parameters including time (1, 4, 8 and 12 h), temperature (400, 450, 500 and 550 °C) and gas mixture ratio (0.05, 0.33, 1 and 3 N₂/H₂). By determining the fatigue strength, surface hardness, compound layer thickness and case depth, the optimum working conditions were determined by using a Taguchi design of experiment. After ion nitriding process, it is aimed to maximize fatigue strength, surface hardness and case depth as well as to minimize compound layer thickness. While the optimum conditions were determined, due to the goals (above aims) more than one being, the trade-off among goals was considered. First of all, each goal was optimised, separately. Then, all the goals were optimised together, considering the priority of the goals, and the optimum results were obtained at 0.05 N₂/H₂ gas mixture ratio, at the temperature of 450 °C and for 12 h process time.     

Harmony search based algorithm for the optimum design of grillage systems to LRFD-AISC

Harmony search based optimum design method is presented for the grillage systems. This numerical technique imitates the musical performance process that takes place when a musician searches for a better state of harmony. Jazz improvisation seeks to find musically pleasing harmony similar to the optimum design process which seeks to find the optimum solution. The design algorithm considers the serviceability and ultimate strength constraints which are implemented from Load and Resistance Factor Design—American Institute of Steel Construction (LRFD-AISC). It selects the appropriate W-sections for the transverse and longitudinal beams of the grillage system out of 272 discrete W-section designations given in LRFD-AISC. This selection is carried out such that the design limitations described in LRFD-AISC are satisfied and the weight of the system is the minimum. Many design examples are considered to demonstrate the efficiency of the algorithm presented.     

Fractional wavelet transform for the quantitative spectral resolution of the composite signals of the active compounds in a two-component mixture

Fractional calculus is a powerful tool that has been applied successfully for the analysis of the complex systems. One interesting example of a complex mixture is given by the multicomponent pharmaceutical samples having constant matrix content. The main aim of this study is to develop a new approach based on the combined use of the fractional wavelet transform (FWT) and the continuous wavelet transform (CWT) in order to quantify atorvastatin (ATO) and amlodipine (AML) in their mixtures without requiring a chemical pretreatment. In the first step, the absorption spectra of the compounds and their samples were processed by the FWT method. In the next step, the CWT approach was applied to the fractional wavelet spectra obtained in the above step. The aim of the application of FWT is data reduction corresponding to the spectra of compounds and their commercial samples. In the following step, the CWT was used for the quantitative resolution of the composite signals of the analyzed compounds. After method validation, the proposed signal processing methods based on the combined use of the FWT and the CWT were successfully applied to the resolution of the composite spectra for the quantitation of atorvastatin (ATO) and amlodipine (AML) in tablets.