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1.
PURPOSE: We investigated Sertoli cells in testicular biopsies with carcinoma in situ (CIS) in respect to cytokeratin expression to elucidate the status of Sertoli cell differentiation adjacent to CIS in human testes. Cytokeratin 18 intermediate filaments indicate a state of undifferentiation usually observed in Sertoli cells of prepubertal testes. MATERIALS AND METHODS: 29 testicular biopsies presenting CIS were investigated by means of immunohistochemistry, using a polyclonal antibody against placental-alkaline phosphatase to detect CIS cells and a monoclonal antibody against human cytokeratin 18 to show expression of cytokeratin 18 intermediate filaments in Sertoli cells. RESULTS: All tubules bearing CIS showed positive cytokeratin expression of Sertoli cells if tubules were devoid of normal germ cells. However, a total of 13 specimen revealed CIS cells together with normal germ cells. In the presence of CIS cells together with round or elongated spermatids, adjacent Sertoli cells did not express cytokeratin immunoreactivity. In the case of combined presence of CIS and spermatogonia and primary spermatocytes, Sertoli cells could be found either immunopositive or immunonegative, and were positive in tubules with CIS and spermatogonia only. CONCLUSIONS: Sertoli cells associated with CIS cells undergo a process of dedifferentiation, seen by the re-expression of cytokeratin intermediate filaments. We suggest that this dedifferentiation results in a loss of Sertoli cell function and leads to a cessation of spermatogenic activity. 相似文献
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Wouter Bergmann Tiest Marcel Bruijn Henk Hoevers Piet de Korte Jan van der Kuur Wim Mels 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》2004,520(1-3):329-332
We have performed numerical calculations of the noise in voltage-biased superconducting transition edge-based X-ray microcalorimeters, using a finite-element model. Details of the model are discussed, as well as results for different absorber geometries. The results are in agreement with an analytical model and show that the amount of internal thermal fluctuation noise can be reduced by using a segmented absorber. The simulation also agrees well with experimental data, which, for our detectors, contain no major unidentified noise sources. Furthermore, the discrepancy between the (small-signal) theoretical and the measured energy resolutions for 5.9 keV X-rays, for our sensors typically a factor of 2, is explained by a more accurate modelling of the sensor responsivity, taking into account the large signal behaviour. 相似文献
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Concluding remarks With this brief exposition of the areas covered in this special double issue on Eurotra, we shall now conclude our introduction. We hope that the volume will achieve its goals, as outlined above, at least to some extent. Certainly, much more could be said about a wide range of topics which have been covered in the lifetime or the project so far. For instance, these article, mainly written by linguists, deliberately neglect the software implementation and environment aspects of the prototype Eurotra Translation System, as well as a number of peripheral tools and components of the system that are available to the user, such as lexical data bases, text-handling mechanisms and the like. The reader interested in aspects of this kind is referred to Raw et al. 1989 for a very brief introduction, and to the Eurotra software team at the Commission of the European Communities (DG XIII, Luxembourg) for more details. However, one goal that we do hope to come close to achieving is to give a fair overview of the Eurotra linguistic theory of translation and the mainstream and sideline formalisms expressing variant versions of it. If we have come anywhere near achieving that, our gratitude is due to the numerous Eurotra colleagues who have supported us in preparing this volume, and to the editor of this journal. 相似文献
6.
A method for the determination of carbon black fillers in peroxide vulcanizates or sulfur vulcanizates of natural rubber (NR, cis-1,4-polyisoprene) by metathesis degradation with 1-octene was developed using the catalyst WCl6? (C2H5)3Al2Cl3. The carbon black was separated from the reaction mixture by centrifuging and determined by weighing. In this connection, investigations were also performed with the catalyst WCl6? C2H5Al2Cl3 which was less suitable for filler determination. The rate of degradation of crosslinked NR with (E)-4-octene was measured. Metathesis reactions of 2-methyl-2-pentene (low-molecular-weight model compound of NR) with 7-tetradecene showed the importance of double bond shift for the degradation of NR. 相似文献
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On Alternating Bending Machine WEBI's Suitability for Determination of Fatigue Characteristics Already in 1966, experimental investigations revealed that the flat bending torsion testing machine ?FLATO”? (Schenck, Darmstadt) doesn't keep constant its torsional moment amplitude in case of specimen's fatigue softening or hardening, respectively. Thus. conclusions concerning the material's fatigue characteristics drawn from such experimental results might therefore be seriously false. Furthermore, also the alternating bending testing machine ?WEBI”? (Schenck, Darmstadt) shows an equivalent behaviour concerning its bending moment amplitude. For this reason. the main findings of a comprehensive experimental study carried out on WEBI with an unalloyed steel C 45 (0.45 mass perccnl C) in different heat treatment conditions are reported in this paper. 相似文献
9.
C.Y. Kuo E.E. Bergmann 《Photonics Technology Letters, IEEE》1991,3(9):823-831
The authors study the second-order distortion when an erbium-doped fiber amplifier (EDFA) is used to amplify the analog optical AM cable TV (CATV) multiple carrier signal from a directly modulated distributed feedback (DFB) semiconductor laser. Experimentally, it was seen that this second order distortion depends critically on the gain of the EDFA fiber amplifier. The authors attribute this distortion to the interaction between the frequency chirping of the DFB laser and the variable gain with wavelength of the amplifier. The authors describe an electronic predistorter that compensates the nonlinearity produced by the DFB-laser-EDFA combination. As a result, the high power advantage of the EDFA can be fully realized in spite of the potential for second-order distortion in the system.<> 相似文献
10.
Fatigue life estimation of graphite/epoxy laminates under consideration of delamination growth 总被引:1,自引:0,他引:1
H. W. Bergmann R. Prinz 《International journal for numerical methods in engineering》1989,27(2):323-341
A closed form approach to the assessment of the fatigue life of graphite/epoxy laminates under cyclic tension–compression loading has been developed. The model is mechanistic and uses cyclic energy release rates for prediction of delamination growth and of critical delamination sizes which induce buckling and the final failure of the laminates. Tests performed with graphite/epoxy specimens of stacking order [0n, ?m]s with severed central plies [?], and of stacking order [02, +45, 02, ?45, 0, 90]s with a central unloaded hole, indicate good correlation between estimated values and observed delamination growth, critical buckling strength of separated plies and load cycles to failure. 相似文献