Detection of Chlamydia trachomatis infections in women by Amplicor PCR: comparison of diagnostic performance with urine and cervical specimens

We used the Roche Amplicor PCR assay to compare urine and cervical swabs as sample material in the detection of Chlamydia trachomatis causing genital infections. The diagnostic performance of Amplicor PCR was compared with that of cell culture and the Gen-Probe PACE 2 assay with cervical specimens. If discrepant from other results, the specimens negative by PCR were diluted and reanalyzed to reveal PCR inhibitors. Of 666 patients, 39 (5.9%) were confirmed to have chlamydial infection. The respective sensitivity and specificity of Amplicor PCR were as follows: urine specimens, 82.0 and 99.7%; cervical specimens, 82.0 and 99.8%. Those for cell culture with cervical specimens were 84.6 and 100%. For the Gen-Probe PACE 2 assay, the sensitivity and specificity with cervical specimens were 79.5 and 100%, respectively. Without the effect of PCR inhibitors, the sensitivity of PCR with urine would have been 97.4%. Provided that the problems currently caused by inhibitors will be solved, the Amplicor PCR assay with urine specimens offers a tempting alternative for the diagnosis of C. trachomatis infection in women.     

Copper and zinc metabolism in aspartylglycosaminuria and Salla disease

Changes in the metabolism of copper and zinc are described in aspartylglycosaminuria (AGU) patients. AGU patients had significantly reduced serum zinc concentrations. However, hair zinc levels were normal, and hyperzincuria could not be demonstrated. The copper content in the hair of AGU patients was highly elevated. Serum copper and ceruloplasmin concentrations were within normal range. In AGU, small-molecular-weight glycoasparagine storage products accumulate in tissues and are excreted in urine in large amounts. They may interfere with the transport mechanisms of trace elements, and thus alter their distribution and availability for tissues. The changes in copper and zinc levels may contribute to the pathogenesis of some of the clinical signs of AGU and Salla disease.     

Significance of Wood Terpenoids in the Resistance of Scots Pine Provenances Against the Old House Borer, Hylotrupes bajulus, and Brown-Rot Fungus, Coniophora puteana

We tested how terpenoid (i.e., monoterpenes and resin acids) composition and concentration in wood affects resistance against wood-borers and decaying fungi. Scots pine (Pinus sylvestris) wood from nine provenances having variable terpenoid profiles was studied against the old house borer, Hylotrupes bajulus, and the decay fungus, Coniophora puteana. Provenances represented a 1200-km N-S transect from Estonia to northern Finland, but they were all cultivated for 7 years in the same nursery field, in central Finland. Mean relative growth rate (MRGR) of small H. hajulus larvae positively correlated with the total monoterpene concentration of wood, and feeding was associated with high proportion of levopimaric+palustric acid in wood. Provenance did not affect the MRGR of small or big larvae, but big larvae consumed more wood and produced more frass on the northern Ylitornio trees than on the southern Rakvere and Ruokolahti trees. Low beta-pinene and total monoterpene concentration and low beta: alpha-pinene ratio in wood were all associated with a high number of eggs. The most northern Muonio provenance was the most favored as an oviposition site, differing significantly from Saaremaa, Tenhola, and Suomussalmi. Wood from Saaremaa, Tenhola, Ruokolahti, and Suomussalmi provenance was most resistant against decay fungus, differing significantly from that of Kinnula provenance. However, decay resistance was not clearly associated with the concentrations of wood terpenoids. These results suggest that monoterpene composition of wood affects resistance against wood-boring Cerambycid beetles, but resistance against wood-decaying fungi is not as clearly associated with wood terpenoids.     

Comparing the Variation of Needle and Wood Terpenoids in Scots Pine Provenances

We determined variation in both the concentration and composition of terpenoids in needles and wood within nine Scots pine (Pinus sylvestris) provenances. Seedlings of different provenances representing a 1200-km N–S transect from Estonia to northern Finland were cultivated in Suonenjoki nursery field, central Finland, for seven years. Growth of seedlings and the number of vertical resin ducts in wood were also determined. -Pinene and 3-carene were the major monoterpenes both in the needles and wood. The total monoterpene concentration was about five times higher in the needles than in the wood. A strong positive correlation was found between proportional quantities of several terpenes of the needles and wood, particularly for 3-carene, sabinene, and terpinolene. The needles contained both labdane-type and tricyclic resin acids, whereas the wood contained only tricyclic ones. The wood had a four times higher tricyclic resin acid concentration than the needles. The highest total monoterpene concentration in the needles and in the wood occurred in the most northern Muonio provenance and in the most southern Saaremaa provenance plants, respectively. The amount of high 3-carene genotype trees decreased among the northern provenances. The wood of the most northern Muonio provenance showed the lowest total resin acid concentration, but provenance did not affect total tricyclic resin acids in the needles. Korpilahti provenance trees from central Finland had the best growth in height. In addition, Korpilahti and Ruokolahti provenance trees showed largest radial growth of stem and smallest number of vertical resin ducts. The results suggest that especially the proportional quantity of 3-carene in the needles could be used in estimating the amount of this compound in the wood and vice versa.     

Usability study of ME2.0

Mobile context-aware applications execute in the background of hosts mobile devices. The applications source process and aggregate hosts’ contextual and personal information. This information is disclosed to ubiquitously pervasive services that adapt their offerings to individual preferences. Unfortunately, many developers continue to ignore the user perspective in context-aware application designs as they complicate their overall task and generate exponential requirements. The additional incorporation of privacy mechanisms in context-aware applications to safeguard context and personal information disclosures also complicates users’ tasks resulting to misconfigured or completely abandoned applications. Misconfigured applications give end-users a false assurance of privacy exposing them to comprising services. We present a usability study on Mobile Electronic Personality Version 2 a privacy enhanced context-aware mobile application for personalising ubiquitous services and adapting pervasive smart-spaces. We draw conclusions on key issues related to user needs, based on user interviews, surveys, prototypes and field evaluations. Users’ needs are evaluated against five themes, learn-ability, efficiency, memorability, errors, satisfaction and privacy contention. In addition, design layout preferences, privacy manageability and consensus design comprehension are also evaluated. Clarity of priorities in context-aware mobile applications shaped by usability studies effectively increases the acceptance of levels of potential users.     

A portable device for intensive care brain function monitoring with event-related potentials

Monitoring level of consciousness or depth of sedation is essential in modern intensive care units and emergency rooms. Current methods are based on spontaneous EEG providing only indirect information on the reactivity of the brain. Measurement of auditory event-related potentials (ERPs) has been shown to have additional potential for evaluation of the level of consciousness. Unfortunately, compact and easy-to-use devices are not commercially available. In this study a portable battery-powered device for clinical auditory ERP measurements was designed, constructed and validated. The device consists of a five-channel data logger and a 16-bit stereo audio stimulator. The signals are digitized with a 22-bit sigma-delta analogue-to-digital converter and stored to a PC Card hard disk. Prior to the in vivo application, the device was validated with extensive technical tests. Importantly, the RMS noise amplitude of the EEG channels was found to be less than 1 mivroV and the delivered auditory stimulus intensity corresponded accurately the settings (mean difference 0.2+/-0.5 dB). In addition to technical tests the device was successfully validated in vivo. To summarize, a novel portable instrument for measurement of auditory event-related potentials in intensive care environment is introduced.     

On the optimality of field-line routing in massively dense wireless multi-hop networks

We consider the load balancing problem in large wireless multi-hop networks by applying the continuum approximation. The task is to find routes, geometric curves, such that the maximal traffic load in the network is minimized. In finite fixed networks, multi-path routes generally yield a lower congestion and thus allow higher throughput. In contrast, we show that in dense wireless multi-hop networks, the optimal load balancing can be achieved by a destination based single-path routing referred to as field-line routing. This is because any routing can be transformed to the corresponding field-line routing with the same or better performance, by using as paths, the field lines of the so-called destination flow associated with the original routing. The concepts are illustrated with two examples. In the case of a unit disk with unit traffic, the maximal load of 0.389 of a multi-path routing system is reduced to 0.343 by using the field-line routing. Similar improvements are also demonstrated for the unit square.     

Non-myopic vehicle and route selection in dynamic DARP with travel time and workload objectives

In dial-a-ride problems, a fleet of n vehicles is routed to transport people between pick-up and delivery locations. We consider an elementary version of the problem where trip requests arrive in time and require an immediate vehicle assignment (which triggers an appropriate route update of the selected vehicle). In this context, a relatively general objective can be stated as a weighted sum of the system's effort and the customers' inconvenience. However, optimizing almost any objective in this immensely complex stochastic system is prohibitively difficult. Thus the earlier work has largely resorted to heuristic cost functions that arise, e.g., from the corresponding static systems. By using the framework of Markov decision processes and the classical M/M/1 queue as a highly abstract model for a single vehicle, we explain why certain intuitive cost functions indeed give satisfactory results in the dynamic system, and also give an explicit interpretation of different components appearing in a general cost function. The resulting family of heuristic control policies is demonstrated to offer a desired type of performance thus justifying the assumed analogy between a multi-queue and dial-a-ride systems.