Genetic control of differential baseline breathing pattern

The purpose of the present study was to determine the genetic control of baseline breathing pattern by examining the mode of inheritance between two inbred murine strains with differential breathing characteristics. Specifically, the rapid, shallow phenotype of the C57BL/6J (B6) strain is consistently distinct from the slow, deep phenotype of the C3H/HeJ (C3) strain. The response distributions of segregant and nonsegregant progeny were compared with the two progenitor strains to determine the mode of inheritance for each ventilatory characteristic. The BXH recombinant inbred (RI) strains derived from the B6 and C3 progenitors were examined to establish strain distribution patterns for each ventilatory trait. To establish the mode of inheritance, baseline breathing frequency (f), tidal volume, and inspiratory time (TI) were measured five times in each of 178 mature male animals from the two progenitor strains and their progeny by using whole body plethysmography. With respect to f and TI, the two progenitor strains were consistently distinct, and segregation analyses of the inheritance pattern suggest that the most parsimonious genetic model for response distributions of f and TI is a two-loci model. In similar experiments conducted on 82 mature male animals from 12 BXH RI strains, each parental phenotype was represented by one or more of the RI strains. Intermediate phenotypes emerged to confirm the likelihood that parental strain differences in f and TI were determined by more than one locus. Taken together, these studies suggest that the phenotypic difference in baseline respiratory timing between male B6 and C3 mice is best explained by a genetic model that considers at least two loci as major determinants.     

High precision in-cylinder gas thermometry using Laser Induced Gratings: Quantitative measurement of evaporative cooling with gasoline/alcohol blends in a GDI optical engine

The first application of Laser Induced Thermal Gratings Spectroscopy (LITGS) for precision thermometry in a firing GDI optical engine is reported. Crank-angle resolved temperature values were derived from LITGS signals generated in fuel vapour with a pressure dependent precision in the range 0.1–1.0% allowing differences in evaporative or charge cooling effects arising from a variety of ethanol and methanol blends with a model gasoline fuel to be quantified. In addition, fluctuations in temperature arising from cyclic variations in compression were directly detected and measured.     

Amino acid analyses of cereal flour proteins

Amino acid analyses have been made on flours of wheat, rye, barley, oats and maize. The overall pattern is sufficiently similar (particularly between rye and barley) to indicate a common ancestor, but significant individual differences occur. Wheat protein differs from the other four in its higher capacity for polar and H-bonds and lower content of salt links. Other factors such as S.S interchange potential may be more important than the pattern of intermolecular forces since the latter does not change much from rye to barley in spite of their rheological differences. The factors for conversion of nitrogen to protein are, in the above order, 5.7, 5.8, 5.8, 5.7, 5.9.     

Crack propagation in ceramics under cyclic loads

Stable crack growth is observed in notched plates of polycrystalline alumina subject to fully compressive far-field cyclic loads at room temperature in a moist air environment andin vacuo. The fatigue cracks propagate at a progressively decreasing velocity along the plane of the notch and in a direction macroscopically normal to the compression axis. The principal failure events leading to this effect are analysed in terms of notch-tip damage under the far-field compressive stress, microcracking, frictional sliding and opening of microcracks, and crack closure. An important contribution to such Mode I crack growth arises from the residualtensile stresses induced locally at the notch-tip when the deformation within the notch-tip process zone leaves permanent strains upon unloading from the maximum nominal compressive stress. It is shown that the phenomenon of crack growth under cyclic compressive stresses exhibits a macroscopically similar behaviour in a wide range of materials spanning the very ductile metals to extremely brittle solids, although the micromechanics of this effect are very different among the various classes of materials. The mechanisms of fatigue in ceramics are compared and contrasted with the more familiar examples of crack propagation under far-field cyclic compression in metallic systems and the implications for fracture in ceramic-metal composites and transformation toughened ceramic composites are highlighted. Strategies for some important applications of this phenomenon are recommended for the study of fracture mechanisms and for the measurement of fracture toughness in brittle solids.     

Action of glutaraldehyde,nitrous acid or chlorine on wheat proteins

The amino acid analyser has been used to determine which amino acid side chains (except that of tryptophan) in wheat proteins are attacked by three reagents. Glutaraldehyde crosslinks gliadin by reacting with lysine and tyrosine residues so that it gives an electrophoretic pattern resembling that of glutenin. Wheat gliadin after treatment with nitrous acid suffers an overall reduction in electrophoretic mobility due to deamination of lysine side chains. Chlorine oxidises part of the cystine and methionine of wheat flour protein to cysteic acid and the sulphone respectively, destroys a fraction of the tyrosine and histidine, and causes some deamidation probably as a result of hydrolysis by hydrochloric acid. The results suggest that there may be buried lysine and tyrosine residues in, and some degrees of structural similarity among, the gliadin proteins. Additional support for the crosslinked nature of glutenin is provided.     

Cardiovascular reactivity during social and nonsocial stressors: Do children's personal goals and expressive skills matter?

The relationships between social competence and cardiovascular reactivity were evaluated in 180 children (ages 8-17; 52% female; 53% Caucasian). Participants performed a social task (Social Competence Interview [SCI]) and 2 nonsocial tasks while reactivity measures were obtained. Social competence was coded from SCI audiotapes. Among adolescents, higher scores on the Acceptance-Affiliation subscale were associated with increased heart rate (HR) and blood pressures and with decreased HR variability during the SCI. Among boys, greater Acceptance-Affiliation scores were associated with increased vascular reactivity during the SCI. During the nonsocial tasks, higher Self-Defensiveness and Expressiveness scores were associated with increased cardiac output and stroke volume among African American children. Personal strivings and expressive skills do matter for understanding cardiovascular responses in children. (PsycINFO Database Record (c) 2010 APA, all rights reserved)