Design of constructions for the expression of proteolytic antibody light chain

A new system for the expression of a catalytic light chain antibody to the vasoactive intestinal peptide is described. The system made possible the isolation the large amounts of a homogeneous protein without any additional peptide domains. The preparation obtained can be used in further experiments on light chain crystallization and in X-ray-structural analysis of its catalytic center.     

Method of calculating forces in buildings subject to bed deformations induced by underground excavations

Basic positions of a procedure for calculating forces in buildings subject to bed deformation induced by underground excavations are presented with, among other things, consideration of the shear stiffness of the building modeled. Examples of calculations are cited for specific structure and bed parameters with the underground excavation residing at different depths. The significance of accounting for the shear stiffness of the structure is established when evaluating the deformations and forces. The potential for use of the method is addressed for the solution of other geotechnical problems. __________ Translated from Osnovaniya, Fundamenty i Mekhanika Gruntov, No. 3, pp. 2–6, May–June, 2007.     

Algorithm and program for engineering analysis of foundation-slab settlement with allowance for nonuniform bed loading and heterogeneity of the mass

An algorithm is proposed for practical implementation of a scheme for analysis of the settlement of building foundations, which has been set forth in modern regulatory documents. Methods to account for a slab with a complex planform, nonuniform loading on the bed, and the actual heterogeneity of the soil bed, which can be assigned directly from survey data, are developed within the framework of this scheme. __________ Translated from Osnovaniya, Fundamenty i Mekhanika Gruntov, No. 5, pp. 2–7, September–October, 2006.     

Identification of Nonstationary Changes in Stress State of Geomaterials by Infrared Radiometry Data

The measurements of intensity of infrared radiation from the surface of rock sample under nonstationary change in stress state are described. The synchronized records of alterations in axial stress of sample under the uniaxial compression and the corresponding time variations in the intensity of infrared radiation from the sample surface are obtained. It is shown that identification of the parameters of elastic oscillations under the action of nonstationary load is reliable.     

Response of anterior parietal cortex to different modes of same-site skin stimulation

Response of anterior parietal cortex to different modes of same-site skin stimulation. J. Neurophysiol. 80: 3272-3283, 1998. Intrinsic optical signal (IOS) imaging was used to study responses of the anterior parietal cortical hindlimb region (1 subject) and forelimb region (3 subjects) to repetitive skin stimulation. Subjects were four squirrel monkeys anesthetized with a halothane/nitrous oxide/oxygen gas mixtures. Cutaneous flutter of 25 Hz evoked a reflectance decrease in the sectors of cytoarchitectonic areas 3b and/or 1 that receive input from the stimulated skin site. The intrinsic signal evoked by 25-Hz flutter attained maximal intensity 相似文献   

Identification of stresses in rocks on the basis of changes in density of infrared radiation flux

The efficiency of infrared radiometry as a source of information on changes in the stress state of rocks is substantiated experimentally. The experiments were conducted under conditions of pulse actions. An analysis of synchronized records of the mechanical state of the specimen and the corresponding variations in the intensity of heat flux from its surface shows the reliability of recording and evaluating “jumps” in stresses on the basis of radiometric data. The Gersevanov Scientific-Research Institute of Foundations and Underground Structures, Moscow. Translated from Fiziko-Tekhnicheskie Problemy Razrabotki Poleznykh Iskopaemykh, No. 6, pp. 48–53, November–December, 1999.     

Measurement of the divergence of pulse-laser radiation by the focal spot method using a mirror wedge

Measurement Techniques -     

SINBAD automation of scientific discovery: From factor analysis to theory synthesis

Modern science is turning to progressively more complex and data-rich subjects, which challenges the existing methods of data analysis and interpretation. Consequently, there is a pressing need for development of ever more powerful methods of extracting order from complex data and for automation of all steps of the scientific process. Virtual Scientist is a set of computational procedures that automate the method of inductive inference to derive a theory from observational data dominated by nonlinear regularities. The procedures utilize SINBAD – a novel computational method of nonlinear factor analysis that is based on the principle of maximization of mutual information among non-overlapping sources, yielding higher-order features of the data that reveal hidden causal factors controlling the observed phenomena. The procedures build a theory of the studied subject by finding inferentially useful hidden factors, learning interdependencies among its variables, reconstructing its functional organization, and describing it by a concise graph of inferential relations among its variables. The graph is a quantitative model of the studied subject, capable of performing elaborate deductive inferences and explaining behaviors of the observed variables by behaviors of other such variables and discovered hidden factors. The set of Virtual Scientist procedures is a powerful analytical and theory-building tool designed to be used in research of complex scientific problems characterized by multivariate and nonlinear relations. This revised version was published online in June 2006 with corrections to the Cover Date.