The double-reversing Z-plasty in primary palatoplasty: operative experience and early results

The double-reversing Z-plasty of Furlow for closure of the soft palate was used in 34 children with various types of cleft palate. Mean age at repair was 12.8 months. Intraoperative experience was favorable, with acceptable operating time and blood loss. Length of hospitalization averaged 1.9 days. Postoperatively, two children experienced temporary stridor, which resolved within 24 to 48 hours. One child had dehiscence of the hard palate (Von Lagenbeck repair) 4 weeks postoperatively, and three children developed small oronasal fistulae. Early speech evaluation demonstrated adequate soft palate mobility in 33 of 34 patients, with observable velopharyngeal function. Twelve children had mild velar compromise, with eight exhibiting slight nasal air escape.     

Rationally designed inhibitors as tools for comparing the mechanism of squalene-hopene cyclase with oxidosqualene cyclase

The inhibition of squalene-hopene cyclase (SHC) (E.C. 5.4.99.-), an enzyme of bacterial membranes catalyzing the formation of pentacyclic sterol-like triterpenes, was studied by using different classes of compounds originally developed as inhibitors of oxidosqualene cyclase (OCS) (E.C. 5.4.99.7), the enzyme of eukaryotes responsible for the formation of tetracyclic precursors of sterols. The mechanism of cyclization of squalene by SHC, beginning with a protonation of the 2,3 double bond by an acidic residue of the enzyme, followed by a series of electrophilic additions of the carbocationic intermediates to the double bonds, is similar to the mechanism of cyclization of 2,3-oxidosqualene by OSC. The inhibitors studied included: (i) analogs of the carbocationic intermediates formed during cyclization, such as aza-analogs of squalene and 2,3-oxidosqualene; (ii) affinity-labeling inhibitors bearing a methylidene reactive group; and (iii) vinyldioxidosqualenes and vinylsulfide derivatives of the substrates. Comparison of the results obtained with the two enzymes, SHC and OSC, showed that many of the most effective inhibitors of OSC were also able to inhibit SHC, while some derivatives acted as specific inhibitors. Differences could be easily explained on the basis of the different substrate specificity of the two enzymes.     

On the detection of card-sharing traffic through wavelet analysis and Support Vector Machines

In the last years, the interest in methods and techniques for circumventing the security of the available digital video broadcasting systems is continuously increasing. Digital TV providers are struggling to restrict access to their video contents only to authorized users, by deploying more and more sophisticated conditional access systems. At the state-of-the-art, the most significant menace is the card-sharing activity which exploits a known weakness allowing an authorized subscriber to provide access to digital contents to a potentially large group of unauthorized ones connected over a communication network. This is usually realized by using ad hoc customized devices. Detecting the presence of these illegal systems on a network, by recognizing their related traffic is an issue of primary importance. Unfortunately, to avoid the identification of such traffic, payload obfuscation strategies based on encryption are often used, hindering packet inspection techniques.This paper presents a strategy for the detection of card-sharing traffic, empowered by machine-learning-driven traffic classification techniques and based on the natural capability of wavelet analysis to decompose a traffic time series into several component series associated with particular time and frequency scales and hence allowing its observation at different frequency component levels and with different resolutions. These ideas have been used for the proof-of-concept implementation of an SVM-based binary classification scheme that relies only on time regularities of the traffic and not on the packet contents and hence is immune to payload obfuscation techniques.     

MOSFET threshold voltage: Definition,extraction, and some applications

This paper exploits a universal current-based definition of the threshold voltage (V_T) and discusses some direct methods to measure it. The consistency, accuracy, and sensitivity of the extraction procedures to second-order effects are examined through numerical simulations and experimental measurements. In addition to three procedures based on dc current measurements we propose an automatic V_T-extractor circuit which allows the direct determination of the threshold voltage with minimum influence of second-order effects.     

Programmable CMOS switched-capacitor biquad using quasi-passivealgorithmic DAC's

This paper describes an electrically programmable switched-capacitor (SC) biquad using quasi-passive algorithmic digital-to-analog converters (DAC's). Since only two equal-valued capacitors are needed for programming each capacitance value, the proposed technique offers compact, cost-effective programmability when compared to traditional programming techniques employing binary-weighted capacitor-arrays (C-arrays). A demonstration prototype chip realized in a 1.2 μm CMOS double-metal double-poly technology, and which implements an 8 b programmable SC biquad giving a wide range of lowpass, bandpass and highpass filtering functions, occupies an active area of only 0.38 mm²     

Isolation and amino acid composition of sauvagine. An active polypeptide from methanol extracts of the skin of the South American frog Phyllomedusa sauvagei

The skin of the South American frog Phyllomedusa sauvagei contains a new active polypeptide, sauvagine, which does not belong to any of the peptide families hitherto described in the amphibian skin. The purification procedure involved several successive steps: dialysis, precipitation with ethanol and acetone, gel filtration and ion exchange chromatography. Two forms of sauvagine were separated. The major component (sauvagine I) was submitted to acid hydrolysis. The sauvagine molecule appeared to possess clear hydrophobic characteristics and to consist of a straight chain of 40 amino acid residues, among which the glutamyl-, aspartyl- leucyl- and isoleucyl-residues were particularly well represented. The elucidation of the amino acid sequence in the sauvagine molecule is in progress.     

Induced maturation of frog mast cells by nerve growth factor during ontogenesis

The effect of nerve growth factor (NGF) on ontogenesis of frog mast cells was investigated in vivo by histochemical, morphometric, and ultrastructural analysis. Three groups of tadpoles at various stages of development were used. In the first group, the larvae received i.p. injections of 1 ng NGF/g; the second group received 10 ng NGF/g, while the control group received only the vehicle. The first recognizable mast cells arose symmetrically in the tongue at stage 26 of Witschi's standard table. At stages 26 and 29, the mast cell number in the NGF-injected tadpoles was significantly higher than the control group. From stage 29 onward, the mast cell number rapidly increased in all groups. No significant differences in mast cell number were observed between the control group and the NGF-injected groups at stages 31 and 33. Electron microscopy revealed that at metamorphic climax (stage 33), the mast cells in the NGF-treated groups were more mature than those in the control group. Therefore, nerve growth factor at early stages of tadpole development is likely to induce differentiation of mast cell precursors, while at later stages it is likely to induce maturation of immature mast cells. The close anatomical association between mast cells and perineurium, observed during nerve development, is intriguing. Already in the early stages of nerve development, the mast cells form a network around Schwann cell-axon complexes, together with the perineurial cells. At climax, the mast cells are located between the perineurial layers, suggesting that they may play a role in the tissue-nerve barrier of the perineurium. Nerve growth factor also seems to induce perineurial cell maturation.     

Web Engineering at the Frontier of the Web 2.0: Design Patterns for Online 3D Shared Spaces

Online 3D Shared Spaces (3DSSs) can be regarded as a frontier of the Web 2.0, where users as participants contribute to create a meaningful, engaging experience. Like other complex web applications, the development and evolution of high-quality 3DSS applications requires methodological support—through models, methods, and principles. Yet, the application of structured, engineered approaches to this domain is largely unexplored. The purpose of this paper is to contribute to bridging Web Engineering to the 3DSS world by means of design patterns. We present five patterns that focus on two factors deemed necessary for effective experiences in a 3DSS: Presence (i.e. the feeling of “being there”, typical of “virtual worlds”) and Long-Term Engagement (typical of successful Web 2.0 communities). The patterns presented in the paper distil our large-scale experiences with 3DSSs (that have involved so far over 9,000 youngsters from 3 continents) and are discussed in the light of existing literature.     

P2P multicast for pervasive ad hoc networks

During the last few years, the proliferation of miniaturised devices with networking capabilities has provided the technological grounds for pervasive networking environments. It is not visionary to foresee a world of pervasive devices embedded in the environment interacting between them, and with those carried by users, via wireless communications. In addition, fostered by the diffusion of small-size, computational-rich mobile devices, the way content is generated, and accessed is changing with respect to the legacy-Internet paradigm. An ever-increasing share of the Internet content is generated directly by the users, and shared on the network (following the User-Generated Content model). While today the legacy Internet is still used to share user-generated content, it is reasonable to envision that pervasive networking technologies will represent the natural platform to support this new model. This will result in content being distributed on users’ devices rather than on centralised servers on the Internet, and in users creating ad hoc networks to share content. The p2p paradigm is particularly suitable for this scenario, because communications will occur directly among users, instead of being necessarily mediated by centralised servers. Motivated by these remarks, in this work we focus on p2p multicast services over ad hoc networks aimed at sharing content among groups of users interested in the same topics. Specifically, starting from a reference solution in legacy wired networks (Scribe), we design a cross-layer optimised protocol (XScribe) that addresses most of the Scribe problems on ad hoc networks. XScribe exploits cross-layer interactions with a proactive routing protocol to manage group membership. Furthermore, it uses a lightweight, structureless approach to deliver data to group members. By jointly using experimental results and analytical models, we show that, with respect to Scribe, XScribe significantly reduces the packet loss and the delay experienced by multicast receivers, and increases the maximum throughput that can be delivered to multicast groups.