ERCM与数据库设计

本文介绍了一种实用的数据库设计方法，它以实体分析法为理论基础，通过对我们日常使用的表进行聚集和归类，完成数据库的逻辑设计，所设计的数据库满足ＢＮＯＦ范式。同时该方法可操作性较强，本文还介绍了自行设计的ＥＲＣＭ数据库辅助设计工具。     

攀钢转炉炼钢用碳化硅提温的试验研究

低品位碳化硅是转炉炼钢的有效提高温剂。工业性试验结果表明，碳化硅加入８．０ｋｇ／ｔ钢水提温４０．３－７０．３℃（平均４８．４℃）；渣中ＴＦｅ降低１．３％；加提温剂有利于转炉内造渣脱硫和碱少炉渣对耐火砖的侵蚀。文中还给出了攀钢转炉半钢冶炼铸用钢不水时加与不加碳化硅的ｔλ，［Ｃ］λ区域。     

Tay-Sachs disease in persons of French-Canadian heritage in northern New England

This study sought to determine whether persons of French-Canadian heritage in northern New England are at high risk for the lethal infantile form of Tay-Sachs disease. In order to accomplish this, death records and laboratory diagnostic records were surveyed to ascertain Tay-Sachs deaths in a cohort of 372,000 live births between 1977-1986. The proportion of the total population with French-Canadian or Jewish heritage was determined from census and birth records, and the ethnic background of Tay-Sachs cases was determined from the corresponding birth records. In 1,860 births, both parents were of Ashkenazi Jewish heritage. One of those children was diagnosed with Tay-Sachs disease. In 41,000 births, both parents were of French-Canadian heritage, and in an additional 93,000 births, one parent was of French-Canadian heritage. No cases of Tay-Sachs disease were identified in the offspring of those individuals. Approximately 14 cases (95% confidence interval 8-20) would be expected, if the gene frequency approximated that reported for individuals of Ashkenazi Jewish heritage. Based on the results of this study, routine testing for Tay-Sachs disease heterozygosity is not indicated for persons of French-Canadian heritage in northern New England. This conclusion may not necessarily be valid for persons of French-Canadian heritage residing in other states. Further studies of Tay-Sachs disease mutations and prevalence among persons of French-Canadian heritage will be important to determine possible regional variations in gene frequencies.     

Membrane topology and glycosylation of the human multidrug resistance-associated protein

The membrane topology of the human multidrug resistance-associated protein (MRP) was examined by flow cytometry phenotyping, immunoblotting, and limited proteolysis in drug-resistant human and baculovirus-infected insect cells, expressing either the glycosylated or the underglycosylated forms of this protein. Inhibition of N-linked glycosylation in human cells by tunicamycin did not inhibit the transport function or the antibody recognition of MRP, although its apparent molecular mass was reduced from 180 kDa to 150 kDa. Extracellular addition of trypsin or chymotrypsin had no effect either on the function or on the molecular mass of MRP, while in isolated membranes limited proteolysis produced three large membrane-bound fragments. These experiments and the alignment of the MRP sequence with the human cystic fibrosis transmembrane conductance regulator (CFTR) suggest that human MRP, similarly to CFTR, contains a tandem repeat of six transmembrane helices, each followed by a nucleotide binding domain, and that the C-terminal membrane-bound region is glycosylated. However, the N-terminal region of MRP contains an additional membrane-bound, glycosylated area with four or five transmembrane helices, which seems to be a characteristic feature of MRP-like ATP-binding cassette transporters.     

Transcytosis of alpha1-acidic glycoprotein in the continuous microvascular endothelium

By using perfusions and bolus administration, coupled with postembedding immunocytochemical procedures, we have identified the structures involved in the transport of derivatized orosomucoid (alpha1-acidic glycoprotein) across the continuous microvascular endothelium of the murine myocardium. Our findings indicate that: (i) monomeric orosomucoid binds to the luminal surface of the endothelium; (ii) it is restricted to caveolae during its transport across the endothelium; (iii) it is detected in the perivascular spaces at early time points (by 1 min) and in larger quantities at later time points (>5 min) from the beginning of its perfusion or its intravascular administration; (iv) no orosomucoid molecules are found in the intercellular junctions or at the abluminal exits of interendothelial spaces; and (v) the vesicular transport of orosomucoid is strongly inhibited by N-ethylmaleimide (>80%). Because, by size and shape, the orosomucoid qualifies as a preferential probe for the postulated small pore system, our results are discussed in relation to the pore theory of capillary permeability.     

Endotoxin-induced platelet aggregation in heparinised equine whole blood in vitro

Endotoxaemia is a leading cause of death among horses. Thrombocytopenia is a common finding in clinical and experimentally-induced cases of endotoxaemia and can lead to coagulopathies, including disseminated intravascular coagulopathy which is usually fatal. In this study it was shown that endotoxin (3 ng ml-1 to 25 micrograms ml-1) can aggregate equine platelets in heparinised whole blood in vitro. The endotoxin-induced aggregation (EIA) was shown to be dependent on the presence of leucocytes in the blood and did not occur when detoxified endotoxin was used, suggesting that lipid A was necessary for the response. Aspirin (1 mmol litre-1) had no effect on EIA whereas apyrase (40 micrograms ml-1) completely abolished it and CV3988 (3 to 30 mumol litre-1) (a competitive antagonist of platelet-activating factor) inhibited the response in a concentration-dependent manner. It is concluded that endotoxin activates equine platelets at low concentrations through an indirect mechanism that involves calcium, leucocytes, adenine nucleotides and platelet-activating factor.     

脂肪酸系列表面活性剂的研究进展

概括和综述了4大类10余个脂肪酸系列表面活性剂的合成、性能和应用现状，重点评述了烷醇酰胺及其衍生物、乙氧基化脂肪酸甲酯、脂肪酸甲酯磺酸盐、N-酰基ED3A、氨基酸、咪唑啉、甜菜碱、双子表面活性剂等脂肪酸系列表面活性剂的研究进展。此系列表面活性剂易生物降解、安全、多功能和高效能。     

Effect of fretting amplitudes on fretting wear behavior of steel wires in coal mines

Given that fretting wear causes failure in steel wires, we carried out tangential fretting wear tests of steel wires on a self-made fretting wear test rig under contact loads of 9 and 29 N and fretting amplitudes ranging from 5 to 180 μm. We observed morphologies of fretted steel wire surfaces on an S-3000N scanning electron microscope in order to analyze fretting wear mecha-nisms. The results show that the fretting regime of steel wires transforms from partial slip regime into mixed fretting regime and gross slip regime with an increase in fretting amplitudes under a given contact load. In partial slip regime, the friction coefficient has a relatively low value. Four stages can be defined in mixed fretting and gross slip regimes. The fretting wear of steel wires in-creases obviously with increases in fretting amplitudes. Fretting scars present a typical morphology of annularity, showing slight damage in partial slip regime. However, wear clearly increases in mixed fretting regime where wear mechanism is a combination of plastic deformation, abrasive wear and oxidative wear. In gross slip regime, more severe degradation is present than in the other regimes. The main fretting wear mechanisms of steel wires are abrasive wear, surface fatigue and friction oxidation.