NITRITE REDUCTASE IN SALMONELLA TYPHIMURIUM

A nitrite reductase was identified and purified 11 -fold from the cytoplasmic fraction of Salmonella typhimurium grown anaerobically with nitrite as the sole nitrogen source. The enzyme required NADH as a cofactor and showed maximum in vitro reductase activity at pH 8.0.
S. typhimurium, grown anaerobically in glucose-limited minimal medium containing peptone and nitrite, showed shorter generation times and increased cell yields in comparison to nitrite-free cultures. The presence of nitrite had no effect on aerobic cultures. The nitrite reductase functions in a dissimilatory manner and appears to be primarily involved in physiological energy generation during anaerobic growth. The nitrite reductase may function to remove excess reducing power in the form of NADH from the cell thus increasing ATP production during the anaerobic fermentation of glucose. The formation and subsequent assimilation of ammonia from nitrite is of secondary importance to the organism.     

Factors Affecting 1-Octen-3-ol in Mushrooms at Harvest and During Postharvest Storage

Harvesting experiments revealed that immature whole mushrooms with closed veils had higher 1-octen-3-0l levels than more mature whole mushrooms with open veils. The l-octen-3-01 content of the gills was higher in immature mushrooms with closed veils, gradually decreased with maturity as the veil opened, and finally increased after the gills were well exposed. Postharvest experiments demonstrated that storage temperature influenced the activity of lipoxygenase-hydroperoxide lyase, but no differences in 1-octen-3-01 content were observed. Enzyme activity as well as 1-octen-3-01 content decreased during storage. Addition of calcium chloride to irrigation water, employed to improve quality and shelf life of fresh mushrooms, increased the amount of l-octen-3-01 immediately after harvest.     

Growth of a rifampicin-resistant strain of a proteolytic psychrotroph,Pseudomonas fluorescens,in raw and ultra heat-treated milk

Pseudomonas fluorescens UQM2490, resistant to 250 μg rifampicin/ml, was derived from P. fluorescens JC1, a proteolytic psychrotroph isolated from raw milk. Growth of UQM2490 was followed in raw and ultra heat-treated milk, by viable counting on rifampicin-containing agar medium. The growth curves obtained demonstrate slower growth in raw milk than in treated milk and the variation in growth with change in inoculum level. Generation times in ultra heat-treated milk ranged from 9.5 to 14.1 h compared with 9.6 to 33 h in raw milk.     

Extending the Fatigue Life of Riveted Coped Stringer Connections

Fatigue cracking occurs at the copes of stringer–floorbeam connections of older, riveted steel bridges. Some cracks are quite long and raise serious questions regarding the remaining fatigue life of the subject bridges. Damage limitation methods (DLMs) have been used to increase the fatigue life of these stringers, but the effectiveness of the DLMs for these riveted connections had never been evaluated by tests. Therefore, tests were conducted to evaluate the fatigue life of coped, riveted stringer–floorbeam connections, and the effectiveness of DLMs. Fatigue cracks in the coped stringer–floorbeam connection were initially developed to establish crack initiation requirements and the rate and extent of crack growth. Once a significant crack was noted, one of several DLMs was applied, and the specimens were retested to determine the effectiveness of the DLM in controlling cracking. These DLMs included the drilled hole, the inserted bolt and the removed rivet methods. The relative effectiveness of the methods is described, and a design procedure is proposed for improving their performance.     

Image processing of electron micrographs of deformed filaments

Electron micrographs of filaments are frequently unsuitable for image processing because they are not perfectly straight. Even minor departures from straightness result in appreciable deterioration of the Fourier transform and analysis of periodicities and subsequent image processing becomes difficult. A procedure is described for recovering the projection of a straight filament from that of an imperfectly straight filament.     

TENSOR-AIDED GEARBOX MODELLING

This paper develops an electrical analogue for a gearbox suitable for use in the time-domain modelling of many electromechanical systems, with tensor methods being used to define the changing conditions in the gearbox brought about by backlash. Unlike most existing models it is compatible with the time-varying and discontinuous models often used to describe the electrical items of a typical system, and it allows for the effects of these items of mechanical non-linearities and discontinuities such as friction and backlash.     

Mouth state: A nuisance variable in preference tests?

An artificial mouth-drying procedure was used to assess the impact of mouth-dryness on the pleasantness of drinks. Experiment 1 showed that mouth dryness increases the pleasantness of 3 °C water more than warmer water (13 °C, 23 °C and 33 °C). Experiment 2 showed that mouth dryness increases the pleasantness of a high acid (3.5g 1000 ml⁻¹) lime drink, but not a medium (1.75g 1000 ml⁻¹) or a low acid (0 g 1000 ml⁻¹) lime drink. In both experiments, elevated saliva flow rates were recorded for those drinks that were regarded as more pleasant in the dry-mouth condition than in the control condition. Shifts in preference may be linked to saliva flow because mouth-wetting drinks may offer greater relief from dry-mouth sensations. Our interpretation implies that an adjustment of palatability assessment procedures, taking into account mouth-state effects, may now be warranted.