Evaluation of Digitally Encoded Layer‐by‐layer Coated Microparticles as Cell Carriers

To obtain more biologically relevant data there is a growing interest in the use of living cells for assaying the biological activity of unknown chemical compounds. Density ‘multiplex’ cell‐based assays, where different cell types are mixed in one well and simultaneously investigated upon exposure to a certain compound are beginning to emerge. To be able to identify the cells they should be attached to microscopic carriers that are encoded. This paper investigates how digitally encoded microparticles can be loaded with cells while keeping the digital code in the microcarriers readable. It turns out that coating the surface of the encoded microcarriers with polyelectrolytes using the layer‐by‐layer (LbL) approach provides the microcarriers with a ‘highly functional’ surface. The polyelectrolyte layer allows the growth of the cells, allows the orientation of the cell loaded microcarriers in a magnetic field, and does not hamper the reading of the code. It has further been shown that the cells growing on the polyelectrolyte layer can become transduced by adenoviral particles hosted by the polyelectrolyte layer. It is concluded that the digitally encoded microparticles are promising materials for use in biomedical and pharmaceutical in‐vitro research where cells are used as tools.     

Structure-activity studies of human tumour necrosis factors

The mechanism by which tumour necrosis factors (TNF and lymphotoxin,also called TNFß and TNFß respectively) exerttheir cytotoxic activity on many malignant cells, remains largelyunknown. Furthermore, the broad array of differentiation (geneinduction) and mitogenic activities towards many primary cellsis still a subject of intensive investigation. TNF is an importantmediator in inflammation, immune responses and infection-relatedphenomena and these activities contribute to the severe toxidtyseen when TNF is used as an anticancer agent. The first stepin the mechanism of action is the specific binding of the ligandto its receptors and dissection of the molecular mechanism involvedin this interaction is the subject of this review. The reasonsfor the interest in this aspect are obvious: first, the developmentof strong antagonistic TNF analogues can be useful in dampeningthe potentially lethal or debilitating effects of an overproductionof the cytokine (as in septic shock or rheumatoid arthritis).Secondly, since two distinct TNF receptors exist, constructionof TNF muteins that distinguish between both types may leadto derivatives of this plekrtropic agent with a more restrictedbiological activity pattern. Ideally, one would like to developa TNF mutant that has retained its cytotoxic action on tumourcells without inducing the deleterious systemic toxteity. Suchan optimized TNF molecule could become a potent anticancer agent     

The forward muon detector of the DELPHI experiment at LEP

The layout and construction of the muon drift chambers equipping the end caps of the DELPHI detector are described. The performance of this forward muon detection system, determined from cosmic ray tests and data collected during the first year of LEP operation, is presented.     

Improving security and performance of an Ad Hoc network through a multipath routing strategy

Privacy and security solutions require today the protection of personal information so that it may not be disclosed to unauthorized participant for illegal purposes. It is a challenge to address these issues in networks with strong constraints such as Ad Hoc network. The security increase is often obtained with a quality of service (QoS) decrease. We propose in this paper a solution that provides at the anonymity, security to Ad Hoc network with a limited impact on QoS. This method could be efficient against some viral attacks. We also give some security proofs of our solution for Ad Hoc networks. The work of Hervé Aiache and Cédric Tavernier was supported by DISCREET, IST project no. 027679, funded in part by the European Commission’s Information Society Technology 6th Framework Programme.     

Airfoil optimisation for vertical‐axis wind turbines with variable pitch

To advance the design of a multimegawatt vertical‐axis wind turbine (VAWT), application‐specific airfoils need to be developed. In this research, airfoils are tailored for a VAWT with variable pitch. A genetic algorithm is used to optimise the airfoil shape considering a balance between the aerodynamic and structural performance of airfoils. At rotor scale, the aerodynamic objective aims to create the required optimal loading while minimising losses. The structural objective focusses on maximising the bending stiffness. Three airfoils from the Pareto front are selected and analysed using the actuator cylinder model and a prescribed‐wake vortex code. The optimal pitch schedule is determined, and the loadings and power performance are studied for different tip‐speed ratios and solidities. The comparison of the optimised airfoils with similar airfoils from the first generation shows a significant improvement in performance, and this proves the necessity to properly select the airfoil shape.     

Low‐Temperature Chemical Synthesis of High‐Purity Diacylglycerols (DAG) from Monoacylglycerols (MAG)

A chemical method was developed for low‐temperature synthesis of DAG from MAG followed by an easy purification procedure in order to obtain high‐purity DAG. Solvent‐assisted and solvent‐free reaction conditions were used, combined with different catalysts (sodium methoxide, p‐toluenesulfonic acid, methanesulfonic acid, and sulfuric acid). All reactions were performed at 35 and 70 °C. By increasing both acidity and polarity of the catalyst the equilibrium shifts towards the formation of DAG. When using sulfuric acid in solvent‐assisted condition at 70 °C, 88 % conversion was obtained after 20 min of reaction (77 % w/w DAG in the reaction mixture after evaporation of the solvent). After purifying by means of column chromatography, 96 % pure DAG were obtained. The overall yield of DAG was 81 %.