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排序方式: 共有264条查询结果,搜索用时 31 毫秒
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Dr. Emrah Kara Dr. Nis Valentin Nielsen Bergrun Eggertsdottir Dr. Bernd Thiede Dr. Sandip M. Kanse Dr. Geir Åge Løset 《Chembiochem : a European journal of chemical biology》2020,21(13):1875-1884
We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was tested with Factor-VII activating protease (WT-FSAP) and its single-nucleotide polymorphism variant Marburg-I (MI)-FSAP. The WT-FSAP results confirmed the previously reported Arg/Lys centered FSAP cleavage site consensus as dominant, as well as reinforcing MI-FSAP as a loss-of-function mutant. Surprisingly, rare substrate clones devoid of basic amino acids were also identified. Indeed one of these peptides was cleaved as free peptide, thus suggesting a broader range of WT-FSAP substrates than previously anticipated. 相似文献
3.
An 8-bit, 200 MSPS Folding and Interpolating ADC 总被引:1,自引:0,他引:1
An 8-bit, 200 MSPS folding and interpolating analog-to-digitalconverter, ADC, has been implemented in a 1.2 µmBiCMOS-process. It achieves 7.5 effective bits with a power dissipationof 575mW. The active area is 4mm2. The implementationand measured results are presented. A simple analytical modelfor the interpolation-induced nonlinearity in a folding and interpolatingADC using sinusoidal folding is presented. The bowing of thereference ladder due to interaction with the input stages isanalyzed, and analytical models are derived. 相似文献
4.
Trond E. Jentoftsen Odd-Arne Lorentsen Ernest W. Dewing Geir M. Haarberg Jomar Thonstad 《Metallurgical and Materials Transactions B》2002,33(6):909-913
The solubility of TiO2 in cryolite-alumina melts at 1020 °C was measured; it decreased with increasing alumina concentration up to ∼3.5 wt pct total
oxide and then increased at higher alumina concentrations. The solubility was found to be 3.1 wt pct Ti in cryolite, and 2.7
wt pct Ti in an alumina-saturated melt. Modeling indicated that the most probable titanium species are TiOF2 and Na2TiO3, which coexist in the solution; the former dominates at low alumina concentrations and the latter at high alumina concentrations.
Additional unknown amounts of fluoride may also be associated with these species. Determination of the solubility of TiO2 in alumina-saturated melts as a function of temperature showed that the solubility increased from 1.9 wt pct Ti at 975 °C
to 2.8 wt pct Ti at 1035 °C, the apparent partial molar enthalpy of dissolution of TiO2 being 88±4 kJ mol−1. 相似文献
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We present a model of a purchaser of electricity in Norway, bidding into a wholesale electricity pool market that operates a day ahead of dispatch. The purchaser must arrange purchase for an uncertain demand that occurs the following day. Deviations from the day-ahead purchase are bought in a secondary market at a price that differs from the day-ahead price by virtue of regulating offers submitted by generators. Under an assumption that arbitrageurs are absent in these markets, we study conditions under which the purchaser should bid their expected demand and examine the two-period game played between a single generator and purchaser in the presence of a competitive fringe. In all our models, it is found that purchasers have an incentive to underbid their expected demand, and so the day-ahead prices will be below expected real-time prices. We also derive conditions on the optimal demand curve that purchasers should bid if the behavior of the other participants is unknown but can be modeled by a market distribution function. 相似文献
7.
Ehsan Rezapour Kristin Y. Pettersen Jan T. Gravdahl Andreas Hofmann 《Artificial Life and Robotics》2016,21(3):282-294
This paper considers formation control of snake robots. In particular, based on a simplified locomotion model, and using the method of virtual holonomic constraints, we control the body shape of the robot to a desired gait pattern defined by some pre-specified constraint functions. These functions are dynamic in that they depend on the state variables of two compensators which are used to control the orientation and planar position of the robot, making this a dynamic maneuvering control strategy. Furthermore, using a formation control strategy we make the multi-agent system converge to and keep a desired geometric formation, and enforce the formation follow a desired straight line path with a given speed profile. Specifically, we use the proposed maneuvering controller to solve the formation control problem for a group of snake robots by synchronizing the commanded velocities of the robots. Simulation results are presented which illustrate the successful performance of the theoretical approach. 相似文献
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We have tested three methods for estimating 2003-2008 elevation changes of Svalbard glaciers from multi-temporal ICESat laser altimetry: (a) linear interpolation of crossover points between ascending and descending tracks, (b) projection of near repeat-tracks onto common locations using Digital Elevation Models (DEMs), and (c) least-squares fitting of rigid planes to segments of repeat-track data assuming a constant elevation change rate. The two repeat-track methods yield similar results and compare well to the more accurate, but sparsely sampled, crossover points. Most glacier regions in Svalbard have experienced low-elevation thinning combined with high-elevation balance or thickening during 2003-2008. The geodetic mass balance (excluding calving front retreat or advance) of Svalbard's 34,600 km2 glaciers is estimated to be −4.3 ± 1.4 Gt y−1, corresponding to an area-averaged water equivalent (w.e.) balance of −0.12 ± 0.04 m w.e. y−1. The largest ice losses have occurred in the west and south, while northeastern Spitsbergen and the Austfonna ice cap have gained mass. Winter and summer elevation changes derived from the same methods indicate that the spatial gradient in mass balance is mainly due to a larger summer season thinning in the west and the south than in the northeast. Our findings are consistent with in-situ mass balance measurements from the same period, confirming that repeat-track satellite altimetry can be a valuable tool for monitoring short term elevation changes of Arctic glaciers. 相似文献
9.
Towards the development of a virtual environment-based training system for mechanical assembly operations 总被引:2,自引:1,他引:1
John E. Brough Maxim Schwartz Satyandra K. Gupta Davinder K. Anand Robert Kavetsky Ralph Pettersen 《Virtual Reality》2007,11(4):189-206
In this paper, we discuss the development of Virtual Training Studio (VTS), a virtual environment-based training system that
allows training supervisors to create training instructions and allows trainees to learn assembly operations in a virtual
environment. Our system is mainly focused on the cognitive side of training so that trainees can learn to recognize parts,
remember assembly sequences, and correctly orient the parts during assembly operations. Our system enables users to train
using the following three training modes: (1) Interactive Simulation, (2) 3D Animation, and (3) Video. Implementing these
training modes required us to develop several new system features. This paper presents an overview of the VTS system and describes
a few main features of the system. We also report user test results that show how people train using our system. The user
test results indicate that the system is able to support a wide variety of training preferences and works well to support
training for assembly operations.
相似文献
Satyandra K. GuptaEmail: |
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