Application of the novel Droplet digital PCR technology for identification of meat species

The authenticity and traceability of meat products are issues of primary importance to ensure food safety. Unfortunately, food adulteration (e.g. the addition of inexpensive cuts to minced meat products) and mislabelling (e.g. the inclusion of meat from species other than those declared) happens frequently worldwide. The aim of this study was to apply a droplet digital PCR assay for the detection and quantification (copies μL⁻¹) of the beef, pork, horse, sheep, chicken and turkey in meat products. The analysis conducted on commercial meat showed the presence of traces of DNA from other animal species than those declared. We show that the method is highly sensitive, specific and accurate (accuracy = 100%). This method could be adopted by competent food safety authorities to verify compliance with the labelling of meat products and to ensure quality and safety throughout the meat supply chain, from primary production to consumption.     

Automated Parallelization of Discrete State-Space Generation

We consider the problem of generating a large state-space in a distributed fashion. Unlike previously proposed solutions that partition the set of reachable states according to a hashing function provided by the user, we explore heuristic methods that completely automate the process. The first step is an initial random walk through the state space to initialize a search tree, duplicated in each processor. Then, the reachability graph is built in a distributed way, using the search tree to assign each newly found state to classes assigned to the available processors. Furthermore, we explore two remapping criteria that attempt to balance memory usage or future workload, respectively. We show how the cost of computing the global snapshot required for remapping will scale up for system sizes in the foreseeable future. An extensive set of results is presented to support our conclusions that remapping is extremely beneficial.     

Overview of health-related quality-of-life measures for pediatric patients: application in the assessment of pharmacotherapeutic and pharmacoeconomic outcomes

Health-related quality of life (HRQOL) is an important dimension in assessing health care. Several methodologic considerations are related to the manner in which these data are obtained in children. Few multidimensional generic measures of quality of life (QOL) have been developed for children and adolescents. Most published research concerns the development of tools to be used in a disease-specific manner for clinical trials. Although several authors point out numerous advantages in assessing HRQOL in clinical practice, several barriers must be overcome for this to occur. In the current era of economic restraint, HRQOL measures must be integrated into pharmaco-economic analyses to assess fully the impact of a drug on health care resources and outcomes.     

Hybrid photocatalytic membranes embedding decatungstate for heterogeneous photooxygenation

The incorporation of decatungstate in polymeric membranes provides new heterogeneous photocatalysts for the oxidation of organic substrates under oxygen atmosphere at 25 °C. Photocatalytic membranes have been prepared yielding polymeric films with a high thermal, chemical and mechanical stability (PVDF, PDMS, Hyflon). Surface spectroscopy techniques including transmittance and reflectance UV-Vis and FT-IR have been used to assess the photocatalyst integrity within the polymeric support. Catalyst screening has been performed under both homogeneous and heterogeneous photooxygenation conditions. The photocatalyst activity has been evaluated in terms of the substrate conversion, turnover numbers, and recycling experiments. A membrane induced selectivity behavior has been evidenced by comparison with homogeneous oxidations.     

Electrocortical desynchronization after microinfusion of kainic acid into the locus coeruleus in rats

CD-1, a genetically-engineered CHO cell line, was cultivated with a Biosilon microcarrier culture system. We successfully cultivated CD-1 cells to a very high density (over 1 x 10(7) cells/ml). Prourokinase was stably secreted at about 180 IU/10(6) cells/24 h. Experiments showed that CD-1 cells growing on Biosilon microcarriers were able to spontaneously release from the microcarriers, then reattach and proliferate on fresh microcarriers. This makes it very easy to scale up production. The microcarriers could be reused several times without affecting adhesion, proliferation and prourokinase secretion. With CM-PECC membrane radial flow chromatography and MPG chromatography, the prourokinase in conditioned medium could be purified to a specific activity of 1 x 10(5) IU/mg of protein. The purification factor was about 600 fold, and approximately 90% of the biological activity was recovered.     

Post-Event Site Investigation,Monitoring, Stability Analysis,and Modeling of a Gas Pipeline Explosion

This paper describes the results of site investigations, monitoring, stability analyses, and soil-pipe interaction modeling of a built-up slope located near Pineto (Abruzzo Province, Central Italy), where a gas pipeline exploded on March 6th, 2015, due to heavy rains inducing slope movements. The slope is formed by OC clay, covered with an upper 10- to 14-m-thick clayey-sandy silt colluvial layer. The explosion in the upper portion of the slope caused extensive damage to existing buildings and threatened human lives. Soon after the event, a site investigation and monitoring program was carried out. A detailed topographic survey and hydrological data were analyzed in order to characterize possible critical rainfall events. The stability of the slope was analyzed both in pre- and in post-explosion conditions. The profiles of the DMT horizontal stress index K _D helped to identify multiple slip surfaces. Then, the results of the site investigation and stability analyses were used to implement a simplified finite element model aimed to describe the soil-pipeline interaction, taking into account the role of the observed wrinkle in the pipeline. The numerical simulations reveal the crucial role played by the slope movements, and by the wrinkle as well, in inducing the collapse of the pipe.     

Anaplastic large cell lymphoma Hodgkin's-like: a randomized trial of ABVD versus MACOP-B with and without radiation therapy

During the last few years, morphological, immunohistochemical, and genetic findings have placed anaplastic large cell lymphoma (ALCL) as a distinct clinicopathologic entity, and several reports have focused on the existence of different subtypes of the tumor. Particular attention has been paid to the ALCL-Hodgkin's-like (HL) subtype, which seems to be on the border between Hodgkin's disease (HD) and high-grade non-Hodgkin's lymphoma (HG-NHL). From September 1994 to July 1997, during the course of an Italian multicentric trial, 40 ALCL-HLs were randomized to receive as front-line chemotherapy MACOP-B (methotrexate with leucovorin, doxorubicin, cyclophosphamide, vincristine, prednisone, and bleomycin-a third-generation HG-NHL regimen) or ABVD (doxorubicin, bleomycin, vinblastine, and dacarbazine-a scheme specific for HD). All patients with bulky disease in the mediastinum at diagnosis underwent local radiotherapy after the chemotherapeutic program. Complete response (CR) was achieved in 17 of the 19 (90%) patients who were treated with MACOP-B, and in 19 of the 21 (91%) patients who were administered ABVD. The probability of relapse-free survival, projected at 32 months, was 94% for the MACOP-B subset and 91% for the ABVD subset. The majority of patients with mediastinal bulky disease obtained CR (evaluated with 67Ga single photon emission computed tomography [SPECT]) after their radiotherapy. The present study suggests that ALCL-HL, in line with its borderline status, responds in an equivalent way to third-generation chemotherapy for HG-NHL and to conventional HD treatment in terms of both CR and relapse-free survival rates. However, as to the latter, a longer follow-up period may be needed before stating the absolute equivalence of the two regimens used.     

Quality and homogeneity monitoring of radiologists in the Turin mammographic screening. Comparison of the pilot phase results

The Turin mammographic screening program was designed to be carried out in several independent screening centers because of the large proportion of population involved (76,000 women aged 50 to 59 years). The first center began working in 1992 and the second center was opened in 1995. The latter center carried out an early pilot study in which 1024 women were examined to assess the homogeneity and the quality of the results. The results were then compared with European quality standards and with the results of both the pilot and the active screening periods in the first center. The results from the second center were very good as far as detection rate is concerned (7.8/1000-2.9/1000 in carcinoma < or = 1 cm). This rate is higher than the so-called "desirable" European standard. Recall rate and benign/malignant biopsy ratio were higher than the so-called "fair" European standard (recall rate: 7.1%; B/M biopsy ratio: 0.62). These results are slightly superior to those of the pilot period in the first center and slightly inferior to the results of active screening in the same center. The improvement relative to the pilot period in the first center (1991) is probably related to technical progress, such as the introduction of the double mammographic projection. The difference relative to the results from the active screening period in the first center (1992-1994) reflects different specific experience. Even though pilot periods have no statistical significance, they can be used for comparison, and in our experience they have proved, with positive results, the quality of the mammographic screening program provided to the Turin population.