Purification and Characterization of β-Glucosidase from Aspergillus niger

Aspergillus niger, an isolate of soil contaminated with effluents from cotton ginning mill was grown in Czapek-Dox medium containing sawdust, Triton-X 100 and urea for production of an extracellular β-glucosidase. β-Glucosidase enzyme was purified (86-fold) from culture filtrate of A. niger by employing ammonium sulphate precipitation and gel filtration on sephadex G-75. The molecular mass of the purified enzyme was estimated to be 95 kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The enzyme had an optimal activity on p-nitrophenyl β-D-glucopyranoside at 50°C and pH 5.0. The K_m and V_max of the enzyme on p-nitrophenyl β-D-glucopyranoside at 50°C and pH 5 were 8.0 mM and 166 µmol/min/mg of protein, respectively. The enzyme could hydrolyze cellobiose and lactose but not sucrose. Heavy metals like Hg²⁺, Al³⁺, and Ag⁺ inhibited the activity, whereas Zn²⁺ and detergents such as Triton-X 100 and Tween-80 increased the activity at 0.01%. The enzyme activity increased in the presence of methanol and ethanol.     

Influence of ZrB2 hard ceramic reinforcement on mechanical and wear properties of aluminum

In this work, the effect of ZrB₂ (0, 5, 10 and 20?vol%) ceramic reinforcement on densification, structure, and properties of mechanically alloyed Al was investigated. The milling of Al-ZrB₂ powder compositions resulted in formation of agglomerates with varied size. In particular, the size of agglomerates was reduced considerably with increased addition of ZrB₂ to Al. Interestingly, the densification of hot pressed Al increased from 96.06% to 99.22% with ZrB₂ addition. The reduction of agglomerates size was attributed to the enhanced densification of Al-ZrB₂ composites. Pure Al showed relatively low hardness (0.94?GPa) and it was improved to 1.78?GPa with the addition of 20?vol% ZrB₂. The mechanical properties have significantly been improved for Al-ZrB₂ composites. Especially Al - 20?vol% ZrB₂ possessed a very high yield strength (529?MPa), compressive strength (630?MPa) and compressive strain of 19.25%. Realization of such a good combination of mechanical properties is the highest ever reported for Al composites so far in the literature. The coefficient of friction (COF) of Al-ZrB₂ varied narrowly between 0.33 and 0.40 after dry sliding wear against steel disc. The wear rate of Al-ZrB₂ composites was within mild wear regime and varied between 98.88?×?10^?6 and 34.66?×?10^?6 mm³/Nm. Among all the compositions, Al - 20?vol% ZrB₂ composite exhibited the lowest wear rate and high wear rate was noted for pure Al. Mild abrasion, tribo-oxidation, third body wear (wear debris) and delamination were the major material removal mechanisms for Al-ZrB₂ composites. Overall the hardness, strength and wear resistance of Al - 20?vol% ZrB₂ composite was improved by 84.3%, 84.3% and 64.2%, respectively when compared to pure Al.     

Flash memory architecture

Flash memories entered the nonvolatile memory scenario only a few years ago, and now these kind of memories are battling to substitute either EEPROM or EPROM. In fact, their peculiarities are becoming quite interesting in present day applications.In system updating, low power consumption, embedded algorithm for program and erase, high density, low cost packages are some of the items which are making the Flash grow in the nonvolatile memory market share.Some words must be spent in explaining what the market is asking of Flash, which are the main applications for these memories, and how their architecture is arranged.The Flash memory cell behaviour will be described, then the fundamental operations (read, program and erase) are explained and some words are used to introduce the redundancy and device testability concept.     

Influence of cerium oxide additive and aloe vera biodiesel on a CI engine

Diesel is the main source of world transportation due to higher combustion efficiency, compliance, consistency and cost-economy. It is also a major contributor to the world prosperity since it is used extensively. Diesel engine’s emissions are the serious hazard to the world environment and it is measured to be the major causes of air pollution. The demand in biofuels for years created a scope for aloe vera into biodiesels. Aloe vera, having higher calorific value vnthan other plant sources used as biodiesels, enhanced us in making another alternative biodiesel, which has lesser emissions and better performance. In this research work, four biodiesel blends from aloe vera oil with cerium oxide additive are explored for their performance and emission characteristics. The results proved B30 (30% biodiesel, 68% diesel and 1% cerium oxide) gives good performance when compared to other blends.     

Real‐time Image‐based Lighting of Microfacet BRDFs with Varying Iridescence

Iridescence is a natural phenomenon that is perceived as gradual color changes, depending on the view and illumination direction. Prominent examples are the colors seen in oil films and soap bubbles. Unfortunately, iridescent effects are particularly difficult to recreate in real‐time computer graphics. We present a high‐quality real‐time method for rendering iridescent effects under image‐based lighting. Previous methods model dielectric thin‐films of varying thickness on top of an arbitrary micro‐facet model with a conducting or dielectric base material, and evaluate the resulting reflectance term, responsible for the iridescent effects, only for a single direction when using real‐time image‐based lighting. This leads to bright halos at grazing angles and over‐saturated colors on rough surfaces, which causes an unnatural appearance that is not observed in ground truth data. We address this problem by taking the distribution of light directions, given by the environment map and surface roughness, into account when evaluating the reflectance term. In particular, our approach prefilters the first and second moments of the light direction, which are used to evaluate a filtered version of the reflectance term. We show that the visual quality of our approach is superior to the ones previously achieved, while having only a small negative impact on performance.     

An optimization technique for simultaneous reduction of PAPR and out-of-band power in NC-OFDM-based cognitive radio systems

Noncontiguous orthogonal frequency division multiplexing (NC-OFDM)-based cognitive radio (CR) systems achieve highly efficient spectrum utilization by transmitting unlicensed users' data on subcarriers of licensed users’ data when they are free. However, there are two disadvantages to the NC-OFDM system: out-of-band power (OBP) and a high peak-to-average power ratio (PAPR). OBP arises due to side lobes of an NC-OFDM signal in the frequency domain, and it interferes with the spectrum for unlicensed users. A high PAPR occurs due to the inverse fast Fourier transform (IFFT) block used in an NC-OFDM system, and it induces nonlinear effects in power amplifiers. In this study, we propose an algorithm called “Alternative Projections onto Convex and Non-Convex Sets” that reduces the OBP and PAPR simultaneously. The alternate projections are performed onto these sets to form an iteration, and it converges to the specified limits of in-band-power, peak amplitude, and OBP. Furthermore, simulations show that the bit error rate performance is not degraded while reducing OBP and PAPR.     

Epilepsy analysis using open source EDF tools for information science and data analytics

Electroencephalogram (EEG) is the signals that measure the electrical variances of brain using metal electrodes. We observe the EEG signals by using European Data Format (EDF) BROWSER and EEG STUDIO. By using EDF BROWSER, we can get the mean and frequency from the filtered output signal using band‐pass filter. Using EDF BROWSER, we can also perform Root Mean Square (RMS) and signal samples. Using EEG STUDIO, we can analyze the average frequency and standard deviation. Epileptic seizure prediction and detection are done by spike detection, frequency domain analysis, and nonlinear methods. EEG signal contains different artifacts like electrooculography (EOG), EKG, and electrocardiogram (ECG). ECG signals are produced by heart. EOG signals are produced by eyes. EMG signals are produced by muscle coordination.     

Wear Mechanisms of TiB2 and TiB2–TiSi2 at Fretting Contacts with Steel and WC–6 wt% Co

Unlubricated fretting wear tests on TiB₂ and TiB₂–5 wt% TiSi₂ ceramics against two different mating materials (bearing grade steel and WC–6 wt% Co balls) were performed with a view to understand the counterbody-dependent difference in friction and wear properties. The fretting experiments were conducted systematically by varying load (2–10 N) at an oscillating frequency of 4 Hz and 100 μm linear stroke, for a duration of 100,000 cycles. Adhesion, abrasion, and three-body wear have been observed as mechanisms of material damage for both the TiB₂/steel and TiB₂/WC–Co tribosystems. The third body is predominantly characterized as tribochemical layer for TiB₂/steel and loose wear debris particles for TiB₂/WC–Co tribocouple. An explanation on differences in tribological properties has been provided in reference to the counterbody material as well as microstructure and mechanical properties of flat materials.     

Determination of the occurrence of Arcobacter butzleri in beef and dairy cattle from Texas by various isolation methods

Arcobacter butzleri is a pathogenic bacterium that has been found in dairy cattle, pigs, poultry, and humans. As of this writing, there are no data on the incidence of A. butzleri in beef cattle. Given the differences in rearing practices used for feedlot cattle and those used for dairy cattle, differences in the incidences of this organism in various types of cattle may also exist. Numerous culture methods have been used to isolate A. butzleri, but there are few data on the comparative efficacies of these methods. The objectives of this study were to determine the incidence of A. butzleri in cattle from Texas and to compare the effectiveness levels of the Johnson-Murano (JM) method (consisting of enrichment in JM broth followed by plating on JM agar) and the Collins method (consisting of enrichment in EMJH-P80 broth followed by plating on Cephalothin, Vancomycin, and Amphotericin B [CVA] agar) in the isolation of this organism. Fifty cattle each from two feedlots, a dairy, and a stocker yard were sampled. Fecal swabs were obtained from cattle, and each sample was cultured by the JM method, the Collins method, and combinations of the two methods with the broth of one method being used with the agar of the other. Polymerase chain reaction was used to identify the isolates for confirmation of A. butzleri. Samples from 18 of 200 cattle tested positive for A. butzleri. This organism was detected by the JM method in 4.5% of the samples and by the Collins method in 2.5% of the samples. An incidence of 4.0% was found when JM broth was used with CVA agar, while no samples tested positive for A. butzleri when EMJH-P80 broth was used with JM agar.     

A static verification approach for architectural integration of mixed-signal integrated circuits

In this paper we present a static method for verifying the proper integration of analog and mixed-signal macroblocks into an integrated circuit. We consider the problem in a setting where there is no golden reference for verifying the validity of the interconnections between the blocks. The proposed verification methodology relies on an abstract modeling of the functional behavior of the blocks and a set of consistency criteria defined over the composition of these abstract models. A new formalism called mode sequence chart (MSeqC) has been presented for capturing the behavior of the blocks at a level of abstraction that is suitable for interconnection verification. We present rules to compose the MSeqCs of each block in an integrated design and present three criteria that indicate possible interconnection faults. We present a tool called AMS-IV (AMS-interconnection verification) that takes the design netlist as input, the MSeqC model of each design block as reference, and tests the three criteria.