Characterization of minocycline loaded chitosan/polyethylene glycol/glycerol blend films as antibacterial wound dressings

Wound care has been a challenging subject for medical teams and researchers. Bacterial infections are one of the most serious complications in injured skins that often affect healing process. Antibacterial wound dressings can be used to facilitate wound healing process. The purpose of this study is to fabricate chitosan (Chito)/polyethylene glycol (PEG) antibacterial wound dressing doped with minocycline, and to evaluate the influence of composition ratio on the blending properties of the films. To improve the mechanical properties of these films, we examined various amounts of glycerol as a plasticizer. Moreover, we investigated morphological and mechanical aspects, water uptake, degradation, water vapor transmission and wettability properties of the films prepared with various ratios of Chito/PEG/Gly. Assessment of mechanical properties revealed that film containing 80:20 ratio Chito/PEG with 40 PHR Gly content exhibits the highest ultimate tensile strength and elongation at break (9.74 MPa and 45.73% respectively). Furthermore, results demonstrated that upon increasing PEG and Gly contents, degradability and hydrophilicity of the films increased whereas water uptake decreased. Water vapor transmission rate of the films was close to the range of 530–1200 g/m²d, indicating that the as formed films are possible candidates for dressing low exudate wounds or burns. Minocycline loaded films exhibited a biphasic drug release profile and it was more effective on gram-positive bacteria than on gram-negative bacteria. The polymeric film with the highest amount of loaded drug (2%) exhibited insignificant cytotoxicity (88%) against normal fibroblast cell line.     

Physical and Morphological Properties of Combined Treated Wood Polymer Composites by Maleic Anhydride and Methyl Methacrylate

Wood polymer composites were prepared by consecutive impregnation with maleic anhydride (MAN) and methyl methacrylate (MMA). Samples impregnated with MAN alone, were heated at 120°C and 150°C for 4 and 8 h. Based on the Fourier transform infrared (FT-IR) analysis and soaking-drying test results, treatment with MAN at 150°C for 4 h resulted in formation of stable crosslinks. In the second stage, MMA was used for in situ polymerization within MAN-treated wood. Field emission scanning electron microscopy observation and FT-IR analysis indicated that MMA copolymerized with MAN, and the resultant polymer filled up the lumen and is also grafted on to the cell wall. Improvement of water repellency and dimensional stability were observed in the treated samples, particularly in combined treated samples. The MAN/MMA treatment improved interaction between polymer and wood.     

Reversible Control of Gelatin Hydrogel Stiffness by Using DNA Crosslinkers**

Biomaterials with dynamically tunable properties are critical for a range of applications in regenerative medicine and basic biology. In this work, we show the reversible control of gelatin methacrylate (GelMA) hydrogel stiffness through the use of DNA crosslinkers. We replaced some of the inter-GelMA crosslinks with double-stranded DNA, allowing for their removal through toehold-mediated strand displacement. The crosslinks could be restored by adding fresh dsDNA with complementary handles to those on the hydrogel. The elastic modulus (G’) of the hydrogels could be tuned between 500 and 1000 Pa, reversibly, over two cycles without degradation of performance. By functionalizing the gels with a second DNA strand, it was possible to control the crosslink density and a model ligand in an orthogonal fashion with two different displacement strands. Our results demonstrate the potential for DNA to reversibly control both stiffness and ligand presentation in a protein-based hydrogel, and will be useful for teasing apart the spatiotemporal behavior of encapsulated cells.     

Clean low-temperature in situ synthesis of durable silver nanoparticles along with aminolysis of polyester fabric using dopamine hydrochloride

Clean Technologies and Environmental Policy - In this study, aminolysis of polyester fabric was carried out along with in situ synthesis of silver nanoparticles in a single-step processing at room...     

Multiobjective shape optimization of speed humps

This paper presents a new approach to the shape optimization of road speed humps. The proposed approach is based on multiobjective genetic optimization of the hump profile while taking into account the separation phenomenon, which occurs when the front tires of the vehicle momentarily lose contact with the road surface. The optimization is carried out for speeds up to twice the authorized speed (throughout this article, the term authorized speed refers to speed limits enforced in speed reduction [bump] areas of the roads) rather than for illegally high speeds as adopted by many of the previous works. A 6-degree of freedom non-linear dynamic model is used to identify the speeds at which separation occurs, and hump profiles associated with these speeds are discarded as infeasible solutions. Three independent objective functions are selected for optimization. They include the maximum vertical acceleration experienced by the driver when crossing the hump below the authorized speed limit (to be minimized), the same vertical acceleration at speeds above the authorized speed (to be maximized), and the ascending ratio of the “speed—vertical acceleration” curve (to be maximized). These objective functions are evaluated for more than 10,000 humps of two popular profile types (sinusoidal and flat top with straight ramps) and optimum profiles for three speed limits of 20, 25, and 30km/h are determined using the multiobjective nondominated sorting genetic algorithm II. As a result, a Pareto front of at least ten optimal points is achieved for each of the two hump profile types. Furthermore, to incorporate the economical aspects of the real-world problem, Pareto optimal points for the two profile types were compared based on their lateral section areas (an indication of the manufacturing cost). The comparison shows that sinusoidal humps more often than not outdo their flat top rivals economically.     

Brain-derived neurotrophic factor enhances function rather than survival of intrastriatal dopamine cell-rich grafts

Brain-derived neurotrophic factor (BDNF) has been shown to promote the survival of dopaminergic neurons from the substantia nigra in cell culture. In order to assess whether a similar survival-promoting effect is present also in vivo, we grafted fetal nigral tissue to the dopamine-depleted striatum of 6-hydroxydopamine-lesioned rats receiving two-week intraventricular infusions or daily intrastriatal injections of BDNF, NGF, or vehicle. When infused chronically at a high dose (12 micrograms/day) into the lateral ventricle, BDNF caused a behavioral syndrome of reduced food and water intake, body weight loss, and locomotor hyperactivity in comparison to NGF- and vehicle-infused graft recipients. NGF-infused graft recipients displayed a transient weight loss during the first week of infusion. At 15 days, amphetamine-induced turning was significantly attenuated to 3% of pregraft values in BDNF-infused recipients, whereas functional graft effects were not present in NGF- or vehicle-infused animals. Survival of tyrosine hydroxylase-immunoreactive graft cells, however, was similar in all treatment groups. Notably, NGF- and BDNF-infusions led to a significant size increase of cholinergic host neurons in the medial septal nucleus and the vertical limb of the diagonal band ipsilateral to the infusion, whereas there was no cholinergic neuron hypertrophy in vehicle-infused animals. Daily intrastriatal injections of BDNF (2 micrograms) produced no weight loss or locomotor hyperactivity, but also enhanced functional graft effects in BDNF-injected, as compared to vehicle-injected animals. Survival rates of grafted tyrosine hydroxylase-immunoreactive cells were, however, similar in both groups.(ABSTRACT TRUNCATED AT 250 WORDS)     

Longitudinal vibration analysis of nanorods with multiple discontinuities based on nonlocal elasticity theory using wave approach

Microsystem Technologies - Discontinuities such as cracks and steps on the length, and the mass of attached buckyball on the tip, of nanoresonators have large effects on nanoresonators natural...     

Microfabricated Biomaterials for Engineering 3D Tissues

Mimicking natural tissue structure is crucial for engineered tissues with intended applications ranging from regenerative medicine to biorobotics. Native tissues are highly organized at the microscale, thus making these natural characteristics an integral part of creating effective biomimetic tissue structures. There exists a growing appreciation that the incorporation of similar highly organized microscale structures in tissue engineering may yield a remedy for problems ranging from vascularization to cell function control/determination. In this review, we highlight the recent progress in the field of microscale tissue engineering and discuss the use of various biomaterials for generating engineered tissue structures with microscale features. In particular, we will discuss the use of microscale approaches to engineer the architecture of scaffolds, generate artificial vasculature, and control cellular orientation and differentiation. In addition, the emergence of microfabricated tissue units and the modular assembly to emulate hierarchical tissues will be discussed.