A proteome analysis of the dorsolateral prefrontal cortex in human alcoholic patients

Alcoholic patients commonly experience cognitive decline. It is postulated that cognitive dysfunction is caused by an alcohol-induced region-selective brain damage, particularly to the prefrontal region, and grey and white matter may be affected differently. We used a proteomics-based approach to compare protein expression profiles of the dorsolateral prefrontal cortex (Brodmann area 9 (BA9)) from human alcoholic and healthy control brains. Changes in the relative expression of 110 protein 'spots' were identified in the BA9 grey matter, of which 54 were identified as 44 different proteins. In our recent article, 60 protein spots were differentially expressed in the BA9 white matter and 18 of these were identified (Alexander-Kaufman, K., James, G., Sheedy, D., Harper, C., Matsumoto, I., Mol. Psychiatry 2006, 11, 56-65). Additional BA9 white matter proteins are identified here and discussed in conjunction to our grey matter results. Thiamine-dependent enzymes transketolase and pyruvate dehydrogenase (E1β ubunit) were among the proteins identified. To our knowledge, this is the first time a disruption in thiamine-dependent enzymes has been demonstrated in the brains of 'neurologically uncomplicated' alcoholics. By identifying protein expression changes in prefrontal grey and white matter separately, hypotheses may draw upon more mechanistic explanations as to how alcoholism causes the structural alterations associated with alcohol-related brain damage and cognitive dysfunction.     

Stiffness-Damping Simultaneous Identification under Limited Observation

A more reliable and stable method, compared to the previous one developed by the present writers, of stiffness-damping simultaneous identification of shear-type building structures is proposed using stationary random records under limited observation. It is shown that when stationary random horizontal accelerations are recorded at the floors just above and below a specific story in a shear building model, the story stiffness and the damping ratio can be identified uniquely. The viscous damping coefficient and the material damping ratio can also be identified simultaneously in a numerical model structure. It is also shown that unfavorable effects by extraneous noises, including measurement noises, due to wind excitation, building facilities, and the motion of the occupants can be eliminated partially by using the present method. The accuracy of the present identification method is investigated through the actual records under limited observation in a base-isolated building.     

Semiexplicit Random Response and Sensitivity of Simple SSI System

For a simplified model of a structure-pile-soil system, semiexplicit forms of the responses to random earthquake inputs are derived together with sensitivity expressions of the random responses with respect to a certain model parameter. Originally this model has a non-tridiagonal stiffness and damping matrices with single earthquake input. It is shown for the first time that independent treatment of the free-field ground and multiple imposition of the free-field ground response into the structure-pile system allow for a transformation of the governing equations so that the final form has tridiagonal stiffness and damping matrices. Closed-form expressions of the inverse of the tridiagonal matrix lead to semiexplicit expressions of the random responses and their sensitivities with respect to a certain model parameter.     

Mechanochemically synthesized ZIF-8 nanoparticles blended into 6FDA-TrMPD membranes for C3H6/C3H8 separation

The mixed matrix membranes (MMMs) consisting zeolitic-imidazolate framework-8 (ZIF-8) nanoparticles in a polymer have been of considerable interest in separation applications. The fillers used are mostly synthesized using the solvothermal method. In this study, the ZIF-8 nanoparticles were synthesized using a solvent-less and salt-free mechanochemical method and were added to 6FDA-TrMPD polyimide to prepare MMMs. The single gas permeation of C₃H₆ and C₃H₈ through the MMMs was investigated. The C₃H₆ permeability and C₃H₆/C₃H₈ ideal selectivity of a 20 wt% mechano-synthesized ZIF-8/6FDA-TrMPD MMM were 70% and 32% higher than those of the neat polymer membrane at 0.1 MPa and 308 K, respectively. The C₃H₆/C₃H₈ separation performance of the mechano-synthesized ZIF-8 MMM was similar to that of the conventional solvothermal-synthesized ZIF-8 MMM. This separation performance was in good agreement with the Maxwell model. Temperature and pressure dependence analyses confirmed that the mechano-synthesized ZIF-8 nanoparticles acted as molecular sieves in the MMMs for the C₃H₆ and C₃H₈ permeation.     

Sintering Metal–Organic Framework Gels for Application as Structural Adhesives

Metal–organic frameworks (MOFs)/coordination polymers are promising materials for gas separation, fuel storage, catalysis, and biopharmaceuticals. However, most applied research on MOFs is limited to these functional materials thus far. This study focuses on the potential of MOFs as structural adhesives. A sintering technique is applied to a zeolitic imidazolate framework-67 (ZIF-67) gel that enables the joining of Cu substrates, resulting in a shear strength of over 30 MPa, which is comparable to that of conventional structural adhesives. Additionally, systematic experiments are performed to evaluate the effects of temperature and pressure on adhesion, indicating that the removal of excess 2-methylimidazole and the by-product (acetic acid) from the sintered material by vaporization results in a microstructure composed of large spherical ZIF-67 crystals that are densely aggregated, which is essential for achieving a high shear strength.     

Developments in asymmetric hydrogenation from an industrial perspective

Examples of developments in asymmetric hydrogenation from various perspectives, in an effort to improve efficiency, are reported. Discussed in this Account are (1) the improved synthesis of BINAP ligands, (2) the design of SEGPHOS ligands for higher enantioselectivity, (3) a new protocol with fewer reaction steps to synthesize beta-aminoesters, and (4) a novel asymmetric hydrogenation mediated by a copper catalyst.     

Effects of support stiffnesses on optimal damper placement for a planar building frame

An efficient and systematic procedure is proposed for finding the optimal damper positioning to minimize the dynamic compliance of a planar building frame. The dynamic compliance is expressed in terms of the transfer function amplitudes of the interstory drifts evaluated at the undamped fundamental natural frequency. The dynamic compliance is minimized subject to a constraint on the sum of the damping coefficients of added dampers. Optimality criteria are derived and the optimal damper positioning is determined via an original steepest direction search algorithm. This algorithm enables one to find an optimal damper positioning sequentially for gradually increasing damper levels. The influences of support-member stiffnesses on the response suppression level and on the optimal damper positioning are also disclosed numerically. Copyright © 1998 John Wiley & Sons, Ltd.