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1.
Xylose reductase (XR) from Neurospora crassa was purified to homogeneity and was found to be specific to NADPH (nicotinamide adenine dinucleotide phosphate). The purified enzyme showed M(r) of 60 and 29 kDa by gel filtration and SDS-PAGE indicating the presence of two subunits. The kinetic mechanism of xylose reductase is 'iso-ordered bi bi'. Inactivation of XR by N-bromosuccinimide (NBS) was found to be biphasic with second-order rate constants of 2.5 x 10(2) and 80 M-1S-1 for the fast (kf) and slow phase (ks), respectively. NADPH protected 90% of XR activity against inhibition by NBS. The fluorescence and circular dichroism (CD) studies revealed that inactivation was not due to gross conformational change in the enzyme. Analysis of the modified Stern-Volmer plot indicated that 49% of the tryptophanyl fluorescence was available for quenching which was completely abolished in the presence of NADPH confirming the involvement of tryptophan at the coenzyme binding site. Experimental evidence presented here serves to implicate the involvement of a tryptophan residue at the low-affinity NADPH binding site and the nature of this site has been assessed by using the hydrophobic probe ANS. 相似文献
2.
Glory H. Anila Vigneswaran C. Jagtap Sujeet S. Shruthi R. Hariharan G. Sriram V. S. Shankar 《Neural computing & applications》2021,33(11):6065-6093
Neural Computing and Applications - “Brain–Computer Interface” (BCI)—a real-life support system provides a way for epileptic patients to improve their quality of life. In... 相似文献
3.
C. V. Jagtap V. S. Kadam T. T. Ghogare Y. A. Inamdar A. A. Shaikh R. S. Mane A. V. Shaikh 《Journal of Materials Science: Materials in Electronics》2016,27(12):12335-12339
The chemical synthesis of pristine and cadmium-doped ZnO powders using a simple, cost-effective at 65 °C is reported and characterized for their structures, optical and morphological studies using X-ray diffraction, UV–visible–Near Infra-Red (UV–Vis–NIR) spectroscopy, scanning electron microscopy measurement techniques where XRD spectra confirm the formation of ZnO and Cd-doped ZnO with hexagonal crystal structure. The particle size of ZnO is reduced on Cd-doping from 16 to 14 nm. Plane-view surface morphology analysis supported for spherical-type crystallites and UV–Vis–NIR spectra reveal shift in the band edge of ZnO after Cd-doping. Photo-degradation study of Methylene Blue dye shows Pristine ZnO degrades dye faster than Cd-doped ZnO. 相似文献
4.
A. T. Yadav P. P. Magar V. S. Kadam C. V. Jagtap C. S. Pawar 《Journal of Materials Science: Materials in Electronics》2016,27(12):12297-12301
Nickel oxide (NiO) thin films have been synthesized by simple and inexpensive chemical bath deposition at low temperature. The synthesized thin films were annealed at 623 K and used for further characterization. Structural and morphological properties of the NiO thin film were characterized using X-ray diffraction and scanning electron microscope (SEM), respectively. The structural study shows the simple cubic formation of NiO thin films with average crystallite size of 9 nm. Honeycomb like surface morphology with porous structure was observed from the SEM study. NiO thin film electrode has been used as a counter electrode in dye sensitized solar cell. Finally, photovoltaic parameters such as short circuit current density (Jsc), open circuit voltage (Voc), Fill Factor (FF) and efficiency (η) have been studied. 相似文献
5.
Fluorinated polyurethane dispersions (FPUDs) were synthesized using HDI isocyanurate, polyester polyol and DMPA with varying concentrations of trifluoro ethanol (TFE) in the range of 0–9 wt%. Further FPUDs were characterized by FT-IR and 1H NMR for structural elucidation. Effects of trifluoro ethanol on the dispersion were evaluated by particle size analyzer. It was observed that with increase in TFE, particle size increases. Topography and contact angle were studied by atomic force microscopy (AFM) and goniometer, respectively. To gain more insight into the formation of roughness, power spectral density (PSD) analysis was carried out. It was found that with increase in TFE content, surface roughness increases due to migration of fluorinating agent on the coating substrate and consequently water repellency increases. Nano indentation of coating revealed that fluorinated coating possesses less stiffness and elastic modulus, due to the presence of fluorine on the coating. Further, coatings were analyzed for thermal properties by thermogravimetry analysis (TGA) and differential scanning calorimetry (DSC) which shows the enhancement in thermal stability and glass transition temperature with increase in TFE content. Anti-graffiti test was carried out which showed improved resistance with increasing fluorine content. 相似文献
6.
7.
Pramod N. Jagadale Shivaji R. Kulal Meghanath G. Joshi Pramod P. Jagtap Sanjay M. Khetre Sambhaji R. Bamane 《Materials Science-Poland》2013,31(2):269-275
Here we report a successful preparation of nanostructured calcium silicate by wet chemical approach. The synthesized sample was characterized by various physico-chemical methods. Thermal stability was investigated using thermo-gravimetric and differential thermal analysis (TG-DTA). Structural characterization of the sample was carried out by the X-ray diffraction technique (XRD) which confirmed its single phase hexagonal structure. Transmission electron microscopy (TEM) was used to study the nanostructure of the ceramics while homogeneous grain distribution was revealed by scanning electron microscopy studies (SEM). The elemental analysis data obtained from energy dispersive X-ray spectroscopy (EDAX) were in close agreement with the starting composition used for the synthesis. Superhydrophilic nature of CaSiO3 was investigated at room temperature by sessile drop technique. Effect of porous nanosized CaSiO3 on early adhesion and proliferation of human bone marrow mesenchymal stem cells (BMMSCs) and cord blood mesenchymal stem (CBMSCs) cells was measured in vitro. MTT cytotoxicity test and cell adhesion test showed that the material had good biocompatibility and promoted cell viability and cell proliferation. It has been stated that the cell viability and proliferation are significantly affected by time and concentration of CaSiO3. These findings indicate that the CaSiO3 ceramics has good biocompatibility and that it is promising as a biomaterial. 相似文献
8.
Girendra Pal Singh Pallavi Vishwas Madiwale Ramanand N Jagtap Ravindra V Adivarekar 《应用聚合物科学杂志》2014,131(19)
With growing environmental awareness, ecological concerns and new legislations, natural fiber‐reinforced plastic composites have received increasing attention during the recent decades. The natural fiber composites have many advantages over traditional glass fiber composites, including lower cost, lighter weight, environmental friendliness, and recyclability. This article reports the findings of the studies done on a new fiber, hitherto unexplored, extracted from Saccharum munja grass. The extracted fibers were further treated using sodium hydroxide to improve its performance in composites. Both treated and untreated fiber‐reinforced composites were prepared by hand lay‐up process using unsaturated polyester resin. Mechanical properties and thermal behavior of the composites were evaluated. The improvement in properties was found for alkali‐treated fiber composites. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40829. 相似文献
9.
10.
Sagar Shankar Jagtap Rupali B. Awhad B. Santosh Pandit B. Vidyasagar 《Microgravity science and technology》2011,23(1):41-48
Microgravity, as a different environment, has been shown to affect plant growth and development (Sievers et al. 1996; Sack 1997). In the present study, effects of slow clinorotation (2 rpm) on growth and chlorophyll content in rice (variety: PRH-10)
seedlings were investigated. Rice seeds were clinorotated continuously for 3, 5 and 7 days under ambient conditions. Root
and shoot lengths and weights of rice seedlings were measured on the third, fifth and seventh day. Chlorophyll was extracted
using N, N-Dimethylformamide (DMF). Absorption and fluorescence spectra of chlorophyll were recorded. Chlorophyll a, chlorophyll
b and total chlorophyll contents were calculated from absorption spectra using Arnon’s method. Results showed an increase
in root and shoot lengths in clinorotated samples. Similar results were obtained for root and shoot weights. Absorption spectra
of chlorophyll showed no shift in the absorption peaks. Chlorophyll content was increased in clinorotated samples as compared
to the controls. Interestingly, the difference between chlorophyll content in control and clinorotated samples decreased as
the number of days of clinorotation increased. Chlorophyll a/b ratio was lowered in clinorotated samples as compared to the
controls. These results suggest that slow clinorotation (2 rpm) affects plant growth and chlorophyll content in rice seedlings. 相似文献