A data-driven generic simulation model for logistics-embedded assembly manufacturing lines

Simulation has been used to evaluate various aspects of manufacturing systems. However, building a simulation model of a manufacturing system is time-consuming and error-prone because of the complexity of the systems. This paper introduces a generic simulation modeling framework to reduce the simulation model build time. The framework consists of layout modeling software and a data-driven generic simulation model. The generic simulation model was developed considering the processing as well as the logistics aspects of assembly manufacturing systems. The framework can be used to quickly develop an integrated simulation model of the production schedule, operation processes and logistics of a system. The framework was validated by developing simulation models of cellular and conveyor manufacturing systems.     

Two-staged guillotine cut,two-dimensional bin packing optimisation with flexible bin size for steel mother plate design

This paper looks into the steel mother plate design problem. A slab, which is an intermediate work in process, is subsequently rolled into a mother plate with the specific dimensions of thickness, length, and width. The mother plate is then cut into customer order plates. As a slab is rolled into a mother plate through a series of horizontal and vertical rolling processes, different-sized mother plates can be generated from a single-slab type. This flexibility allows for the size of a mother plate to be determined according to the order plates assigned to it. Furthermore, when the order plates are cut from a mother plate, a guillotine cut is required to reduce the production cost. The steel mother plate design problem involves the placing of order plates on the mother plates in a guillotine cut pattern and determining the sizes of the mother plates with the objective of minimising the number of slabs; thus it may be considered as a two-staged guillotine cut, two-dimensional bin packing problem with flexible bin size. This paper introduces the problem, presents several mathematical models, and proposes an iterative two-phase heuristic method consisting of several algorithms to solve the problem. Computational results for the benchmark problems show the effectiveness of the proposed method.     

Activatable Photosensitizers: Agents for Selective Photodynamic Therapy

Recent developments in the design of bifunctional and activatable photosensitizers rejuvenate the aging field of photodynamic sensitization and photodynamic therapy. While systematic studies have uncovered new dyes that can serve as potential photosensitizers, the most promising results have come from studies aimed at gaining precise control over the location and rate of cytotoxic singlet oxygen generation. As a consequence, higher selectivities and efficiencies in photodynamic treatment protocols are now within reach. This feature article highlights the variety of approaches that have been pursued to improve photodynamic therapy and to transform simple photosensitizers into smarter theranostic agents.     

Integrity and cell-monolayer permeability of chitosan nanoparticles in simulated gastrointestinal fluids

The objective of this study was to assess the integrity and intestinal permeability of chitosan nanoparticles (CNPs) in simulated gastrointestinal fluids (SGIF). The cell-associated and transported chitosan was quantified and visualized after incubation of the CNPs with Caco-2 cell monolayer with/without SGIF treatment. In order to establish the role of proteins in the SGIF, CNPs were incubated with 4 proteins having different isoelectric points (pI). CNPs incubated with the fluids did not attach to the cell monolayer in contrast to the intact CNPs. Negatively-charged protein formed the complex with CNPs leading to particle size increase, but protected CNPs from disintegration. In contrary, positively-charged protein interacted with cross-linker causing disintegration of CNPs. CNPs incubated with the fluids did not attach to the cell monolayer in contrast to the intact CNPs. The results suggested that the surface charges of CNPs and proteins play a critical role in structural changes of CNPs in biological environment.     

Chemosensors for pyrophosphate

The selective detection of the anion pyrophosphate (PPi) is a major research focus. PPi is a biologically important target because it is the product of ATP hydrolysis under cellular conditions, and because it is involved in DNA replication catalyzed by DNA polymerase, its detection is being investigated as a real-time DNA sequencing method. In addition, within the past decade, the ability to detect PPi has become important in cancer research. In general, the sensing of anions in aqueous solution requires a strong affinity for anions in water as well as the ability to convert anion recognition into a fluorescent or colorimetric signal. Among the variety of methods for detecting PPi, fluorescent chemosensors and colorimetric sensors for PPi have attracted considerable attention during the past 10 years. Compared with the recognition of metal ions, it is much more challenging to selectively recognize anions in an aqueous system due to the strong hydration effects of anions. Consequently, the design of PPi sensors requires the following: an understanding of the molecular recognition between PPi and the binding sites, the desired solubility in aqueous solutions, the communicating and signaling mechanism, and most importantly, selectivity for PPi over other anions such as AMP and ADP, and particularly phosphate and ATP. This Account classifies chemosensors for PPi according to topological and structural characteristics. Types of chemosensors investigated and reported in this study include those that contain metal ion complexes, metal complexes combined with excimers, those that function with a displacement approach, and those based on hydrogen-bonding interaction. Thus far, the utilization of a metal ion complex as a binding site for PPi has been the most successful strategy. The strong binding affinity between metal ions and PPi allows the detection of PPi in a 100% aqueous solution. We have demonstrated that carefully designed receptors can distinguish between PPi and ATP based on their different total anionic charge densities. We have also demonstrated that a PPi metal ion complex sensor has a bioanalytical application. This sensor can be used in a simple and quick, one-step, homogeneous phase detection method in order to confirm DNA amplification after polymerase chain reaction (PCR).     

Colloidal Photonic Crystal Strain Sensor Integrated with Deformable Graphene Phototransducer

Flexible, architectured, photonic nanostructures such as colloidal photonic crystals (CPCs) can serve as colorimetric strain sensors, where external applied strain leads to a noticeable color change. However, CPCs' response to strain is difficult to quantify without the use of optical spectroscopy. Integration of flexible electrical readout of CPCs' color change is a challenge due to a lack of flexible/stretchable electrical transducers. This work details a colorimetric strain sensor with optoelectrical quantification based on an integrated system of CPCs over a crumpled graphene phototransducer, which optoelectrically quantifies CPCs, response to strain. The hybrid system enables direct visual perception of strain, while strain quantification via electrical measurement of the hybrid system outperforms that of crumpled graphene strain sensors by more than 100 times. The unique combination of a photonic sensing element with a deformable transducer will allow for the development of novel, electrically quantifiable colorimetric sensors with high sensitivity.     

Cancer‐Associated,Stimuli‐Driven,Turn on Theranostics for Multimodality Imaging and Therapy

Advances in bioinformatics, genomics, proteomics, and metabolomics have facilitated the development of novel anticancer agents that have decreased side effects and increased safety. Theranostics, systems that have combined therapeutic effects and diagnostic capabilities, have garnered increasing attention recently because of their potential use in personalized medicine, including cancer‐targeting treatments for patients. One interesting approach to achieving this potential involves the development of cancer‐associated, stimuli‐driven, turn on theranostics. Multicomponent constructs of this type would have the capability of selectively delivering therapeutic reagents into cancer cells or tumor tissues while simultaneously generating unique signals that can be readily monitored under both in vitro and in vivo conditions. Specifically, their combined anticancer activities and selective visual signal respond to cancer‐associated stimuli, would make these theranostic agents more highly efficient and specific for cancer treatment and diagnosis. This article focuses on the progress of stimuli‐responsive turn on theranostics that activate diagnostic signals and release therapeutic reagents in response to the cancer‐associated stimuli. The present article not only provides the fundamental backgrounds of diagnostic and therapeutic tools that have been widely utilized for developing theranostic agents, but also discusses the current approaches for developing stimuli‐responsive turn on theranostics.     

NapWell: An EOG-based Sleep Assistant Exploring the Effects of Virtual Reality on Sleep Onset

Virtual Reality - We present NapWell, a Sleep Assistant using virtual reality (VR) to decrease sleep onset latency by providing a realistic imagery distraction prior to sleep onset. Our proposed...     

Impact of Incision of Gravel‐bed Rivers on Ground Beetle Assemblages

The impact of river incision induced by channelization and gravel mining on the structure of ground beetle assemblages in riparian habitats was investigated on three montane rivers in southern Poland. Ground beetles were collected on three benches of different elevation in 11 incised and 14 vertically stable cross sections of the rivers. In total, 5821 individuals representing 106 species were collected. The effect of river incision on the diversity and abundance of ground beetles depended on bench height. Only on the lowest bench, inundated about once per year on average, species richness of the assemblages was significantly reduced in incised river cross sections. On this bench, the abundance of the specialists of exposed riverine sediments, i.e. small and medium‐sized predators with high dispersal power and spring breeding strategy, was highly negatively affected by river incision. On the highest bench, large, brachypterous species with spring and autumn breeding strategy, typical of undisturbed habitats, were more abundant in incised cross sections. As this bench is practically not subjected to flooding even in vertically stable cross sections, these species probably benefited from the occurrence of riparian forest along most incised river sections, whereas the riparian areas along vertically stable sections are subjected to higher agricultural pressure. This study shows that in the mountain region where high precipitation helps to maintain moisture of the riparian habitats, river incision has a negative impact only on the specialists of exposed riverine sediments. Copyright © 2016 John Wiley & Sons, Ltd.