Sperm metabolism is altered during storage by female insects: evidence from two-photon autofluorescence lifetime measurements in bedbugs

We explore the possibility of characterizing sperm cells without the need to stain them using spectral and fluorescence lifetime analyses after multi-photon excitation in an insect model. The autofluorescence emission spectrum of sperm of the common bedbug, Cimex lectularius, was consistent with the presence of flavins and NAD(P)H. The mean fluorescence lifetimes showed smaller variation in sperm extracted from the male (tau m, τ_m = 1.54–1.84 ns) than in that extracted from the female sperm storage organ (tau m, τ_m = 1.26–2.00 ns). The fluorescence lifetime histograms revealed four peaks. These peaks (0.18, 0.92, 2.50 and 3.80 ns) suggest the presence of NAD(P)H and flavins and show that sperm metabolism can be characterized using fluorescence lifetime imaging. The difference in fluorescence lifetime variation between the sexes is consistent with the notion that female animals alter the metabolism of sperm cells during storage. It is not consistent, however, with the idea that sperm metabolism represents a sexually selected character that provides females with information about the male genotype.     

Fair‐Weather Friends: Evidence of Lipoxin Dysregulation in Neurodegeneration

Lipoxins (LXs) are autacoids, specialized proresolving lipid mediators (SPMs) acting locally in a paracrine or autocrine fashion. They belong to a complex superfamily of dietary small polyunsaturated fatty acid (PUFA)–metabolites, which direct potent cellular responses to resolve inflammation and restore tissue homeostasis. Together, these SPM activities have been intensely studied in systemic inflammation and acute injury or infection, but less is known about LX signaling and activities in the central nervous system. LXs are derived from arachidonic acid, an omega‐6 PUFA. In addition to well‐established roles in systemic inflammation resolution, they have increasingly become implicated in regulating neuroinflammatory and neurodegenerative processes. In particular, chronic inflammation plays a central role in Alzheimer's disease (AD) etiology, and dysregulated LX production and activities have been reported in a variety of AD rodent models and clinical tissue samples, yet with complex and sometimes conflicting results. In addition, reduced LX production following retinal injury has been reported recently by the authors, and an intriguing direct neuronal activity promoting survival and homeostasis in retinal and cortical neurons is demonstrated. Here, the authors review and clarify this growing literature and suggest new research directions to further elaborate the role of lipoxins in neurodegeneration.     

A two-dimensional time-domain finite element formulation for periodic structures

A formulation is presented for a two-dimensional time-domain finite-element method (FEM-TD) that incorporates periodic boundaries. The specifics of the method are shown for scattering problems, but it should be straightforward to extend it to radiation problems. The method solves for a transformed field variable (instead of solving directly for the electric field) in order to easily enable periodic boundary conditions in the time domain. The accuracy and stability of the method is demonstrated by a series of examples where the new formulation is compared with reference solutions. Very accurate results are obtained when the excitation (frequency range) and the geometry are such that no higher order Floquet modes are present. The accuracy is degraded in the presence of higher order modes due to the rather simple absorbing boundary condition that is used with the present formulation. The method is found to be stable even for angles of incidence close to grazing.     

NUMERICAL ANALYSIS OF THE EFFECTS OF LOCAL HYDRODYNAMICS ON MASS TRANSFER IN HETEROGENEOUS POROUS MEDIA

Many engineering problems require the estimation of mass transfer coefficients in porous materials. In heterogeneous materials or in cases where mass transfer sites are not spatially uniform, empirical equations for mass transfer coefficients vary widely, and the origin of these differences is not well understood. In this article, we use a stochastic algorithm to model mass transfer from single particles in a two-dimensional heterogeneous packed bed. The computed mass transfer coefficients are used to generate a distribution of local Peclet numbers in the bed. Detailed hydrodynamics are then used to interpret variations in the local Peclet number. The results show clear relationships between pore structure, streamline patterns, and mass transfer rates.     

QoS enhancement for adaptive streaming services over WCDMA

This paper presents a new method to adapt the quality-of-service (QoS) in a wideband code-division multiple-access (WCDMA) system, called radio network feedback (RNF). The concept of RNF is in general applicable to all the services requiring a minimum quality (i.e., non best effort) and in this paper, the focus is on streaming. In this context, RNF makes it possible for a streaming server to adapt its source bit rate to a WCDMA radio link, whose bandwidth may vary in time due, for example, to decongestion/congestion situations over the air interface or to handover. This is very beneficial for the end user as it allows to increase the bandwidth (i.e., the quality) when possible and to decrease it (instead of just dropping the service) when needed. RNF was compared with Client-based adaptation solutions. Simulation results show that RNF is fast and accurate and performs better than Client-based adaptation. For example, rebuffering occurs only with Client-based method. Moreover, with RNF the up-switch is performed only when needed, whereas the up-switch performed by means of the Client-based method is often erroneous and may annoy the end user.     

Pheromonal secretions from glands on the 5th abdominal sternite of hydropsychid and rhyacophilid caddisflies (Trichoptera)

Extracts of different body parts of adult Trichoptera were tested for electrophysiological activity. Extracts of the IVth and Vth abdominal sternites of femaleHydropsyche angustipennis, Rhyacophila nubila, andR. fasciata, containing a paired exocrine gland, elicited significant electroan-tennographic responses when tested on conspecific male antennae. The paired gland occurs also in males of all the species, and inH. angustipennis, extracts from males were more active than female extracts when tested on male antennae. Female and male extracts from all species were analyzed by gas chromatography with simultaneous flame ionization and electroantennographic detection (EAD). EAD-active peaks in female extracts, stimulating male antennae, were identified inH. angustipennis as nonan-2-one; and inR. nubila andR. fasciata as heptan-2-one, heptan-2-ol, nonan-2-one, and nonan-2-ol. EAD-active components from maleH. angustipennis stimulating male antennae were octan-2-one, nonan-2-one (major peak), (Z)-6-nonen-2-one, decan-2-one, and a methylbranched decan-2-one. Female extracts and synthetic mixtures of compounds identified from femaleH. angustipennis andR. fasciata were tested for attractivity in the field. High catches with control traps obscured the results, but a synthetic mixture of the four identified compounds was significantly attractive and not different from female extracts for attracting maleR. fasciata. InH. angustipennis, a synthetic six-component male blend, in which nonan-2-one was the major component, attracted significant numbers of male and femaleH. angustipennis. Extracts of maleR. nubila andR. fasciata contained acetophenone and hexanoic and octanoic acids but did not have any electrophysiological or behavioral activity on either male or female antennae of conspecifics. The occurrence of a female sex pheromone inRhyacophila and an aggregation pheromone inHydropsyche corresponds to earlier described differences in mating behaviors in the Rhyacophilidae and Hydropsychidae.     

The Polymorphism of Low Erucic Rapeseed Oil with High Content of Palmitic Acid

A new type of low erucic acid rapeseed oil containing high amount (11%) of palmitic acid (C₁₆-LOBRA) was studied, both in labscale and in pilot-plant equipment. The polymorphic behaviour of fat blends and margarine emulsions consisting of C₁₆-LOBRA, LOBRA and soybean oil and their hydrogenated fats were examined. The results clearly show the potential in the hydrogenated C₁₆-LOBRA. The time before the stable ß-form is developed is much longer for the hydrogenated C₁₆-LOBRA compared with the hydrogenated LOBRA and in fact similar to a hydrogenated soybean oil. The hydrogenated C₁₆-LOBRA is therefore well suited to be used in e.g. margarine systems.     

Chemical inhibitors when timing is critical: a pharmacological concept for the maturation of T cell contacts

Cellular signal transduction proceeds through a complex network of molecular interactions and enzymatic activities. The timing of these molecular events is critical for the propagation of a signal and the generation of a specific cellular response. To define the timing of signalling events, we introduce the combination of high-resolution confocal microscopy with the application of small-molecule inhibitors at various stages of signal transduction in T cells. Inhibitors of Src-family tyrosine kinases and actin dynamics were employed to dissect the role of the lymphocyte-specific tyrosine kinase Lck in the formation and maintenance of T cell receptor/CD3-dependent contacts. Anti-CD3epsilon-coated coverslips served as a highly defined stimulus. The kinetics of the recruitment of the yellow fluorescent protein-tagged signalling protein ZAP-70 were detected by high-resolution confocal microscopy. The analysis revealed that at 5 min after receptor engagement, Lck activity was required for maintenance of contacts. In contrast, after 20 min of receptor engagement, the contacts were Lck-independent. The relevance of the timing of inhibitor application provides a pharmacological concept for the maturation of T cell-substrate contacts.     

Zur Analyse von Triglyceriden

Analysis of Triglycerides Only recently the analytic of triglycerides of natural fats and oils has found in HPLC an adaquate method. By means of HPLC it is possible to separate the glycerides of the fat with respect to partition number (PN) and to analyse the collected fractions by GLC with respect to carbon number (CN). This procedure yields a complete basis for calculation of the composition of triglyceride groups which are characterized by their carbon number and number of double bonds (e.g. C54:1). If the analytical data of each fraction are completed with the fatty acid composition, the fatty acid combination of the single triglycerides and their distribution can likely be characterized. This method was applied to triglyceride analysis of LOBRA oil. The obtained results are reported.