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1.
Venkataraman H Beer SB Bergen LA Essen Nv Geerke DP Vermeulen NP Commandeur JN 《Chembiochem : a European journal of chemical biology》2012,13(4):520-523
Inversion of stereoselectivity: screening of a minimal mutant library revealed a cytochrome P450?BM3 variant M01?A82W?S72I capable of producing 16?α-OH-testosterone. Remarkably, a single active site mutation S72I in M01?A82W inverted the stereoselectivity of hydroxylation from 16?β to 16?α. Introduction of S72I mutation in another 16?β-OH-selective variant M11?V87I, also resulted in similar inversion of stereoselectivity. 相似文献
2.
Hybrid Paper–Plastic Microchip for Flexible and High‐Performance Point‐of‐Care Diagnostics 下载免费PDF全文
Mohamed Shehata Draz Maryam Moazeni Manasa Venkataramani Harini Lakshminarayanan Ecem Saygili Nivethitha Kota Lakshminaraasimulu Kamyar Mehrabi Kochehbyoki Manoj Kumar Kanakasabapathy Shirin Shabahang Anish Vasan Mohamad Ali Bijarchi Adnan Memic Hadi Shafiee 《Advanced functional materials》2018,28(26)
A low‐cost and easy‐to‐fabricate microchip remains a key challenge for the development of true point‐of‐care (POC) diagnostics. Cellulose paper and plastic are thin, light, flexible, and abundant raw materials, which make them excellent substrates for mass production of POC devices. Herein, a hybrid paper–plastic microchip (PPMC) is developed, which can be used for both single and multiplexed detection of different targets, providing flexibility in the design and fabrication of the microchip. The developed PPMC with printed electronics is evaluated for sensitive and reliable detection of a broad range of targets, such as liver and colon cancer protein biomarkers, intact Zika virus, and human papillomavirus nucleic acid amplicons. The presented approach allows a highly specific detection of the tested targets with detection limits as low as 102 ng mL?1 for protein biomarkers, 103 particle per milliliter for virus particles, and 102 copies per microliter for a target nucleic acid. This approach can potentially be considered for the development of inexpensive and stable POC microchip diagnostics and is suitable for the detection of a wide range of microbial infections and cancer biomarkers. 相似文献
3.
Computer viruses have been around since the mid 1980s. Over 40,000 different viruses have been cataloged so far and the number of viruses is increasing dramatically. The damage they cause is estimated to be several billions of U.S. dollars per year. Most often, the origin of the virus is difficult to trace. Various kinds of anti-virus software have been developed which detect viruses and take corrective actions. The anti-virus software needs to be continually updated to cope with newer types of viruses. The proliferation of the Internet and Web, have enabled viruses to spread quickly on a massive scale, by taking advantage of several security loopholes. The continual challenge is to have quick and effective responses to these virus attacks 相似文献
4.
The fate and intermediary metabolism of stearic acid 总被引:1,自引:0,他引:1
Coming from the Greek for “hard fat”, stearic acid represents one of the most abundant FA in the Western diet. Otherwise known
as n-octadecanoic acid (18∶0), stearate is either obtained in the diet or synthesized by the elongation of palmitate, the principal
product of the FA synthase system in animal cells. Stearic acid has been shown to be a very poor substrate for TG synthesis,
even as compared with other saturated fats such as myristate and palmitate, and in human studies stearic acid has been shown
to generate a lower lipemic response than medium-chain saturated FA. Although it has been proposed that this may be due to
less efficient absorption of stearic acid in the gut, such findings have not been consistent. Along with palmitate, stearate
is the major substrate for the enzyme stearoyl-CoA desaturase, which catalyzes the conversion of stearate to oleate, the preferred
substrate for the synthesis of TG and other complex lipids. In mice, targeted disruption of the stearoyl-CoA desaturase-1
(SCD1) gene results in the generation of a lean mouse that is resistant to diet-induced obesity and insulin resistance. SCD1
also has been shown to be a key target of the anorexigenic hormone leptin, thus underscoring the importance of this enzyme,
and consequently the cellular stearate-to-oleate ratio, in lipid metabolism and potentially in the treatment of obesity and
related disorders. 相似文献
5.
A new automata model Mr,k, with a conceptually significant innovation in the form of multi-state alternatives at each instance, is proposed in this study. Computer simulations of the Mr,k, model in the context of feature selection in an unsupervised environment has demonstrated the superiority of the model over similar models without this multi-state-choice innovation. 相似文献
6.
We consider the suitability of the Java concurrent constructs for writing high-performance SPMD code for parallel machines. More specifically, we investigate implementing a financial application in Java on a distributed-memory parallel machine. Despite the fact that Java was not expressly targeted to such applications and architectures per se, we conclude that efficient implementations are feasible. Finally, we propose a library of Java methods to facilitate SPMD programming. © 1997 John Wiley & Sons, Ltd. 相似文献
7.
8.
NiFe2O4 powders were prepared by combustion synthesis, polyol-mediated and sol–gel methods. Morphological characterization of sintered disks was carried out using scanning electron microscopy (SEM). Dielectric properties of NiFe2O4 synthesized by different routes were investigated over the frequency range of 100 Hz–5 MHz at room temperature. A difference in dielectric constant (εr) and dissipation factor (tan δ) of NiFe2O4 samples obtained by different synthesis methods has been observed. The observed dissimilarity in the behavior originates from the variation in the microstructure of the samples that is evident in the Cole–Cole plot results. 相似文献
9.
A Framework for Functional Specification and Transformation of Hidden Surface Elimination Algorithms
This paper deals with the theory of hidden surface elimination algorithms in Computer Graphics. A set of functions and abstract data types are defined to help concisely specify a class of hidden surface elimination algorithms in a purely functional language. A formal study of these algorithms is presented here along with theorems of equivalence between some of the specifications. It is shown here that such proofs of equivalence will help in the construction of existing as well as new algorithms. We bring in the importance of such a study to exploit alternative parallel architectures for implementation of these algorithms. The other benefits of formal specification and analysis are due to its use as a teaching aid and effective method for rapid prototyping of these algorithms. 相似文献
10.
Lakshminarasimhan Harini Bose Karthikeyan Sweta Srivastava Srinag Bangalore Suresh Cecil Ross Georgepeter Gnanakumar Srinivasan Rajagopal Krishnan Sundar Thandavarayan Kathiresan 《IET nanobiotechnology / IET》2017,11(1):57
Breast cancer accounts for the first highest mortality rate in India and second in world. Though current treatment strategies are effectively killing cancer cells, they also end in causing severe side effects and drug resistance. Curcumin is a nutraceutical with multipotent activity but its insolubility in water limits its therapeutic potential as an anti‐cancer drug. The hydrophilicity of curcumin could be increased by nanoformulation or changing its functional groups. In this study, curcumin is loaded on mesoporous silica nanoparticle and its anti‐cancer activity is elucidated with MCF‐7 cell death. Structural characteristics of Mobil Composition of Matter ‐ 41(MCM‐41) as determined by high‐resolution transmission electron microscopy (HR‐TEM) shows that MCM‐41 size ranges from 100 to 200 nm diameters with pore size 2–10 nm for drug adsorption. The authors found 80–90% of curcumin is loaded on MCM‐41 and curcumin is released efficiently at pH 3.0. The 50 µM curcumin‐loaded MCM‐41 induced 50% mortality of MCF‐7 cells. Altogether, their results suggested that increased curcumin loading and sustained release from MCM‐41 effectively decreased cell survival of MCF‐7 cells in vitro.Inspec keywords: cancer, cellular biophysics, nanoparticles, nanomedicine, biomedical materials, polymers, mesoporous materials, transmission electron microscopy, drugs, adsorptionOther keywords: polyethylenimine‐modified curcumin‐loaded mesoporus silica nanoparticle, MCF‐7 cell line, breast cancer, cancer cells, drug resistance, multipotent activity, therapeutic potential, anticancer drug, mesoporous silica nanoparticle, MCF‐7 cell death, high‐resolution transmission electron microscopy, drug adsorption, curcumin‐loaded MCM‐41, nutraceutical curcumin, size 2 nm to 10 nm, size 100 nm to 200 nm 相似文献