Design and Characterization of a New pVII Combinatorial Phage Display Peptide Library for Protease Substrate Mining Using Factor VII Activating Protease (FSAP) as Model

We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was tested with Factor-VII activating protease (WT-FSAP) and its single-nucleotide polymorphism variant Marburg-I (MI)-FSAP. The WT-FSAP results confirmed the previously reported Arg/Lys centered FSAP cleavage site consensus as dominant, as well as reinforcing MI-FSAP as a loss-of-function mutant. Surprisingly, rare substrate clones devoid of basic amino acids were also identified. Indeed one of these peptides was cleaved as free peptide, thus suggesting a broader range of WT-FSAP substrates than previously anticipated.     

Variability in the proliferative responsiveness of cultured human vascular smooth muscle cells to alpha-thrombin

alpha-Thrombin, a key enzyme of the coagulation cascade, is also a potent mitogen for many cell types. In the present study, the responsiveness to alpha-thrombin of cultured human vascular smooth muscle cells (HVSMC) derived from either vein or normal and atherosclerotic arteries was investigated. All HVSMC populations examined responded mitogenically to alpha-thrombin. However, the extent of this response varied between different cell populations. No significant differences were observed between HVSMC derived from vein versus artery or atherosclerotic versus normal tissues. The responsiveness of a specific HVSMC culture to alpha-thrombin was not affected by cell density and remained constant over several passages. Unlike platelet-derived growth factor BB (PDGF-BB), alpha-thrombin did not exhibit any significant chemotactic effects on HVSMC or induce their anchorage independent growth in semi-solid medium. The hypothesis that the observed variability in HVSMC responsiveness to alpha-thrombin is due to the heterogeneity of cultured HVSMC is raised and discussed.     

Seeing and Being Seen: The Effects on Gesture Production

Speakers are argued to adapt their language production to their addressee's needs. For instance, speakers produce fewer and smaller hand gestures when interlocutors cannot see each other. Yet is this because speakers know their addressee cannot see them, or because they themselves do not see their addressee? By means of computer‐mediated communication we manipulated these factors independently. We found that speakers took into account what their addressee saw. They produced more and larger gestures when they knew the addressee could see them. Seeing the addressee increased gesture production only if speakers could readily interpret the addressee's eye gaze, which is not usually the case in mediated interaction. Adding this affordance resulted in gesturing being similar in mediated and unmediated communication.     

A longitudinal study of the social and academic competence of economically disadvantaged bilingual preschool children.

This longitudinal study was conducted to gain understanding of the social–emotional and academic development of economically disadvantaged bilingual preschool children. In Study 1, the authors combined cognitive, psychosocial, and cultural-linguistic factors to determine profiles of social competence as measured by peer play. A person-centered analysis of 207 Hispanic American preschoolers (ages 4 and 5 years) yielded 6 distinct profiles, 2 of which were socially competent and 1 of which was vulnerable. Findings revealed profile differences in social competence and a significant relationship between bilingualism and social–emotional development. In Study 2, the authors determined which profiles were associated with later academic achievement and growth of English proficiency. Findings indicated a significant relationship of early social–emotional development to later academic success and English acquisition, highlighting the role of bilingualism. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Cross-language postevent misinformation effects in Spanish–English bilingual witnesses.

Three experiments examined whether postevent misinformation affects eyewitness memory across languages in bilingual witnesses. Participants watched a videotaped simulation of a robbery, read a postevent narrative that contained misleading information about the robbery, and answered either forced-choice or cued-recall questions about 6 target items from the videotape. Experiment I was conducted entirely in English, but Experiments 2 and 3 tested Spanish–English bilingual participants who were exposed to 1 of 3 language combinations of the postevent narrative and memory test—Spanish–Spanish, English–Spanish, or Spanish–English (as well as English–English in Experiment 3). Across all 3 experiments, the effects of postevent misinformation were as large in the cross-language conditions as in the same-language conditions. This study has important implications for the justice system, and the results suggest new ways to study postevent misinformation effects and bilingual memory. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Specificity of the T-cell responses in covalently linked peptides each comprising of a T helper epitope

The selection of T-cell specificities in chimeric peptides comprising of two T helper epitopes (288-302 and 240-252) from the fusion protein of measles virus was investigated. The resulting chimeric peptides (288-P-240 and 240-P-288) were shown to be immunogenic by inducing proliferative responses in both B10.s and C57BL/6 strains of mice. In B10.s mice immunization with the chimeric peptides resulted in proliferative T-cell responses only to the constituent 288-302 peptide, whereas in C57BL/6 mice no proliferative responses to the constituent 288-302 or 240-252 peptides were detected. In vivo competition studies between the 288-302 and 240-252 peptides for binding to the I-As molecule have shown that the peptide 288-302 was dominant in B10.s mice and competed with the non-dominant 240-252 peptide for the induction of an in vivo response. The absence of any proliferative T-cell response to the constituent 288-302 and 240-252 peptides after immunization of C57BL/6 mice with the 288-P-240 or 240-P-288 chimeric peptides, suggests that the dominant T-cell responses might have shifted to newly formed T cell epitope(s) as a result of the covalent linkage of the two peptides. In conclusion, these results indicate that the selection of Th epitopes within chimeric peptides is dependent not only on the amino acid composition of the epitope but also on the context of the epitope within the chimera and the haplotype of the mouse strain used.     

Propriétés de la Surface d'un Film de Polyéthylène Modifiée par Greffage d'un Oligomère Acrylique

The grafting of a liquid acrylic derivative (2-methylenepentane-1,5-dicarboxylic acid diethylester) on the surface of a polyethylene film (HD) was carried out in the presence of benzoylperoxide. The wettability of the surface was investigated with respect to the adhesion properties against aluminium and compared with those of a film of polyethylene grafted in mass with acrylic acid. The mobility of the surface grafted chains may cause the differences in the observed comportment.     

Minority stress and mental health among Dutch LGBs: Examination of differences between sex and sexual orientation.

Minority stress is often cited as an explanation for greater mental health problems among lesbian, gay, and bisexual (LGB) individuals than heterosexual individuals. However, studies focusing on sex or sexual orientation differences in level of minority stress and its impact on mental health are scarce, even more so outside the United States. Performing secondary analyses on the data of a Dutch population study on sexual health, the present study examines the robustness of the minority stress model by explaining mental health problems among men and women with mostly or only same-sex sexual attraction, and men and women who are equally attracted to same-sex and opposite-sex partners in the “gay-friendly” Netherlands (N = 389; 118 gay men, 40 bisexual men, 184 lesbian women, and 54 bisexual women). Results showed that minority stress is also related to mental health of Dutch LGBs. Participants with a higher level of internalized homonegativity and those who more often encountered negative reactions from other people on their same-sex sexual attraction reported more mental health problems. Such negative reactions from others, however, had a stronger link with mental health among lesbian/gay than among bisexual participants. Openness about one's sexual orientation was related to better mental health among sexual minority women, but not among their male counterparts. Suggestions for future research, implications for counseling, and other societal interventions are discussed. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

FSAP Protects against Histone-Mediated Increase in Endothelial Permeability In Vitro

Factor-VII-activating protease (FSAP) is involved in the regulation of hemostasis and inflammation. Extracellular histones play a role in inflammation and the conversion of latent pro-FSAP into active FSAP. FSAP has been shown to regulate endothelial permeability, but the mechanisms are not clear. Here, we have investigated the effects of FSAP on endothelial permeability in vitro. A mixture of histones from calf thymus stimulated permeability, and the wild-type (WT) serine protease domain (SPD) of FSAP blocked this effect. WT–SPD–FSAP did not influence permeability on its own, nor that stimulated by thrombin or vascular endothelial growth factor (VEGF)-A₁₆₅. Histones induced a large-scale rearrangement of the junction proteins VE-cadherin and zona occludens-1 from a clear junctional distribution to a diffuse pattern. The presence of WT–SPD–FSAP inhibited these changes. Permeability changes by histones were blocked by both TLR-2 and TLR4 blocking antibodies. Histones upregulated the expression of TLR-2, but not TLR-4, in HUVEC cells, and WT–SPD–FSAP abolished the upregulation of TLR-2 expression. An inactive variant, Marburg I (MI)–SPD–FSAP, did not have any of these effects. The inhibition of histone-mediated permeability may be an important function of FSAP with relevance to sepsis, trauma, and stroke and the need to be investigated further in in vivo experiments.     

Are engineering schools masculine and authoritarian? The mission statements say yes.

After reading the mission statements of 20 engineering and liberal arts schools, business students recorded their impressions of a hypothetical successful student at each institution. Based only on institutional mission statements, engineering students were deemed significantly more likely to be dominant, forceful, and masculine and significantly less likely to defend their beliefs, or to be soft spoken, eager to soothe feelings, feminine, or likable. Additionally, for male subjects, the higher their own level of authoritarianism, the more likely they were to indicate that a successful student at an engineering institution was someone like themselves. Authoritarianism had no such predictive value for female subjects. Our results may illustrate why engineering schools might be having trouble attracting a more diverse group of students: a culture of masculinity and hierarchy may be so deeply entrenched that it is evident even to casual observers. (PsycINFO Database Record (c) 2010 APA, all rights reserved)