Reconstruction of cellular biological structures from optical microscopy data

Developments in optical microscopy imaging have generated large high-resolution data sets that have spurred medical researchers to conduct investigations into mechanisms of disease, including cancer at cellular and subcellular levels. The work reported here demonstrates that a suitable methodology can be conceived that isolates modality-dependent effects from the larger segmentation task and that 3D reconstructions can be cognizant of shapes as evident in the available 2D planar images. In the current realization, a method based on active geodesic contours is first deployed to counter the ambiguity that exists in separating overlapping cells on the image plane. Later, another segmentation effort based on a variant of Voronoi tessellations improves the delineation of the cell boundaries using a Bayesian formulation. In the next stage, the cells are interpolated across the third dimension thereby mitigating the poor structural correlation that exists in that dimension. We deploy our methods on three separate data sets obtained from light, confocal, and phase-contrast microscopy and validate the results appropriately.     

Damage Tolerant Magnesium Metal Matrix Composites: Influence of Reinforcement and Processing

A growing impetus to enhance our understanding of the behavior of magnesium-based alloys for use in weight critical applications resulted as a consequence of the low density of magnesium. In an attempt to enhance the applicability of magnesium for a wide spectrum of performance-critical applications, the addition of reinforcement to the alloy was considered as an economically affordable and potentially viable scientific alternative. In this paper are reported the results of a study aimed at understanding the influence of saffil alumina short fiber reinforcement on microstructural development of a squeeze-cast magnesium alloy. Preliminary results confirm promise of the reinforced alloy, which retains hardness, strength, and stiffness better at elevated temperatures compared to the unreinforced counterpart. However, impact strength and toughness of the reinforced alloy are inferior. The importance of the matrix alloy in governing the overall mechanical response of the composite microstructure is discussed based on fractographic observations. The importance of volume fraction of the reinforcing phase on properties of the composite microstructure is highlighted.     

Auto-Discovering Configurations for Service Management

Determining service configurations is essential for effective service management. In this paper we describe a model-driven approach for service configuration auto-discovery. We develop metrics for performance and scalability analysis of such auto-discovery mechanisms. Our approach addresses several problems in auto-discovery: specification of what services to discover, how to efficiently distribute service discovery, and how to match instances of services into related groups. We use object-oriented models for discovery specifications, a flexible bus-based architecture for distribution and communication, and a novel multi-phased, instance matching approach. We have applied our approach to typical e-commerce services, Enterprise Resource Planning applications, like SAP, and Microsoft Exchange services running on a mixture of Windows and Unix platforms. The main contribution of this work is the flexibility of our models, architecture and algorithms to address discovery of a multitude of services.     

New Algorithms for Resource Reclaiming from Precedence Constrained Tasks in Multiprocessor Real-Time Systems

The scheduling of tasks in multiprocessor real-time systems has attracted many researchers in the recent past. Tasks in these systems have deadlines to be met, and most of the real-time scheduling algorithms use worst case computation times to schedule these tasks. Many resources will be left unused if the tasks are dispatched purely based on the schedule produced by these scheduling algorithms, since most of the tasks will take less time to execute than their respective worst case computation times. Resource reclaiming refers to the problem of reclaiming the resources left unused by a real-time task when it takes less time to execute than its worst case computation time. In this paper, we propose two algorithms to reclaim these resources from real-time tasks that are constrained by precedence relations and resource requirements, in shared memory multiprocessor systems. We introduce a notion called a restriction vector for each task which captures its resource and precedence constraints with other tasks. This will help not only in the efficient implementation of the algorithms, but also in obtaining an improvement in performance over the reclaiming algorithms proposed in earlier work [[2]]. We compare our resource reclaiming algorithms with the earlier algorithms and, by experimental studies, show that they reclaim more resources, thereby increasing the guarantee ratio (the ratio of the number of tasks guaranteed to meet their deadlines to the number of tasks that have arrived), which is the basic requirement of any resource reclaiming algorithm. From our simulation studies, we demonstrate that complex reclaiming algorithms with high reclaiming overheads do not lead to an improvement in the guarantee ratio.     

Nano-AlN particle reinforced Mg composites: microstructural and mechanical properties

In this study, nano-AlN particles were introduced into pure Mg matrix through the powder metallurgy technique incorporating microwave assisted two-directional sintering followed by hot extrusion. The effect of varying volume fraction of nano-AlN addition on the microstructural and mechanical properties of pure Mg was investigated. Microstructural characterisation revealed marginal grain refinement due to the fairly uniform distribution of AlN nano-particulates. X-ray diffraction results indicated basal texture weakening in Mg/0·2AlN composite. Tensile property measurements revealed an overall increase in strength properties and ductility. Among the developed composites, Mg/0·8AlN displayed superior strength (～30% improvement) and Mg/0·2AlN showed enhanced ductility (～80% enhancement). Under compressive loading, the developed Mg/AlN nanocomposite formulations exhibited improved strength properties without significant effect on compressibility.     

Classification and Uncertainty Visualization of Dendritic Spines from Optical Microscopy Imaging

Neuronal dendrites and their spines affect the connectivity of neural networks, and play a significant role in many neurological conditions. Neuronal function is observed to be closely correlated with the appearance, disappearance and morphology of the spines. Automatic 3‐D reconstruction of neurons from light microscopy images, followed by the identification, classification and visualization of dendritic spines is therefore essential for studying neuronal physiology and biophysical properties. In this paper, we present a method to reconstruct dendrites using a surface representation of the dendrite. The 1‐D skeleton of the dendritic surface is then extracted by a medial geodesic function that is robust and topologically correct. This is followed by a Bayesian identification and classification of the spines. The dendrite and spines are visualized in a manner that displays the spines' types and the inherent uncertainty in identification and classification. We also describe a user study conducted to validate the accuracy of the classification and the efficacy of the visualization.     

Value of reversed saphenous vein in minimally invasive direct coronary artery bypass graft procedures

BACKGROUND: Minimally invasive direct coronary artery bypass graft procedures are gaining acceptance for revision as well as primary coronary revascularization. When suitable, the left and right internal mammary arteries are preferred as bypass conduits; in other cases, the greater saphenous vein, used for standard coronary artery bypass graft procedures, may be useful to revascularize coronary artery branches during minimally invasive direct coronary artery bypass graft procedures. METHODS: We used the greater saphenous vein on three occasions during minimally invasive direct coronary artery bypass graft procedures (1) to revascularize the left anterior descending coronary artery by anastomosis to the left axillary artery in the infraclavicular region, (2) as an extension to the left internal mammary artery to reach the left anterior descending coronary artery, and (3) as a bridge from the splenic artery to bypass the distal right coronary artery. RESULTS: Postoperatively, all 3 patients had relief from symptoms of coronary artery insufficiency and none has been readmitted to the hospital with symptoms. Angiography or thallium studies were not performed to confirm graft patency because all patients were elderly and the risks of these procedures were considered to outweigh their potential benefit. CONCLUSIONS: The greater saphenous vein is a potential bypass conduit for use in minimally invasive direct coronary artery bypass graft procedures as well as for coronary artery bypass graft procedures.     

Influence of oxygen pressure on structural,optical and magnetic properties of pure ZnO films

In this paper, we study the influence of oxygen pressure on structural, optical and magnetic properties of pure ZnO films. The chemical compositions and thickness of the film were estimated by Rutherford backscattering spectrometry measurements. X-ray diffraction patterns show all the films are in single phase and preferred along (002) orientation. With an increase of oxygen pressure, grain growth and average root mean square roughness is found to be increased. It is found that the intensity of UV emission peak increases whereas visible emission peak decreases in intensity with an increase of oxygen pressure. From the magnetization measurements, it is observed that ZnO film grown without oxygen pressure shows an enhanced ferromagnetic behaviour than that of the films grown with oxygen pressure of 0.05 and 0.1 mbar.     

Properties of squeeze cast Mg-6Zn-3Cu alloy and its saffil alumina short fibre reinforced composites

In the present work, Mg-Zn-Cu alloy (ZC63) and its saffil alumina short fibre reinforced composites produced using the squeeze casting technique were evaluated for their properties. The unreinforced base alloys and their composites were characterized for their microstructure, hardness, yield strength, impact strength, wear resistance and corrosion resistance. The dependence of the properties of composites was studied as a function of fibre volume fraction. Results showed that the composites exhibited improved hardness, yield strength at elevated temperature and wear resistance in comparison to the monolithic alloy. However, ductility, impact strength and corrosion resistance of the composites were inferior to that of the base alloy. The nature of the base alloy matrix in determining the properties of the composites was discussed based on fractographic analysis.     

A comparative and mechanistic study on regioselective photooxidative demethylation of N‐heterocyclics using TiO2 and molecular oxygen

Regioselective photooxidative demethylation of methyl‐substituted N‐containing heterocyclic compounds is investigated in non‐aqueous (ethane nitrile) solutions containing semiconductor oxide (TiO₂) as photocatalyst in the presence of molecular oxygen upon irradiation at room temperature conditions. A plausible electron transfer mechanism, in which an electron‐hole pair is generated on the surface of TiO₂ by illumination, is proposed for the semiconductor‐mediated photocatalysis. Molecular orbital calculation by the AM 1 method has been performed for the electron densities of N‐atoms of heterocyclics in the present study. © 2002 Society of Chemical Industry