Co‐condensation synthesis of well‐defined mesoporous silica nanoparticles: effect of surface chemical modification on plasmid DNA condensation and transfection

Chemically modified mesoporous silica nanoparticles (MSNs) are of interest due to their chemical and thermal stability with adjustable morphology and porosity; therefore, it was aimed to develop and compare the MCM‐41 MSNs functionalised with imidazole groups (MCM‐41‐Im) to unmodified (MCM‐41‐OH) and primary amine functionalised (MCM‐41‐NH₂) MSNs for experimental gene delivery. The results show efficient transfection of the complexes of the plasmid and either MCM‐41‐NH₂ or MCM‐41‐Im. Furthermore, following transfection of HeLa cells using MCM‐41‐Im, an enhanced GFP expression was achieved consistent with the noticeable DNase1 protection and endosomal escape properties of MCM‐41‐Im using carboxyfluorescein tracer.Inspec keywords: condensation, mesoporous materials, silicon compounds, nanoparticles, DNA, surface chemistry, porosity, gene therapy, cellular biophysics, biomedical materials, nanomedicine, nanofabrication, molecular biophysics, biochemistryOther keywords: co‐condensation synthesis, surface chemical modification, plasmid DNA condensation, plasmid DNA transfection, chemical modified mesoporous silica nanoparticles, chemical stability, thermal stability, adjustable morphology, porosity, MCM‐41 MSN functionalisation, imidazole groups, MCM‐41‐OH, primary amine functionalised MSN, gene delivery, HeLa cell transfection, GFP expression, DNase1 protection, endosomal escape properties, carboxyfluorescein tracer, SiO₂      

Optimistic Selection of Cluster Heads Based on Facility Location Problem in Cluster-Based Routing Protocols

Cluster-based routing protocols are one of the most favorable approaches for energy management in wireless sensor networks. The selection of the best cluster heads (CHs), as well as the formation of optimal clusters, is an NP-hard problem. The present study proposes an optimal solution for CHs selection to generate a network topology with optimized network performance. The problem is formulated as facility location problem and a linear programming model is used to solve the optimization problem. Results of analysis o the network simulator (NS2) indicate that applying this method in cluster-based routing protocols prolongs 16 % of the network lifetime, increases 15.5 % of data transmission and improves 5.5 % of throughput, as compared to the results of current heuristic methods such as LEACH, DEEC and EDFCM protocols.     

A review of simulation methods for human movement dynamics with emphasis on gait

Multibody System Dynamics - Human gait analysis is a complex problem in biomechanics because of highly nonlinear human motion equations, muscle dynamics, and foot-ground contact. Despite a large...     

Cell-laden microengineered pullulan methacrylate hydrogels promote cell proliferation and 3D cluster formation

The ability to encapsulate cells in three-dimensional (3D) environments is potentially of benefit for tissue engineering and regenerative medicine. In this paper, we introduce pullulan methacrylate (PulMA) as a promising hydrogel platform for creating cell-laden microscale tissues. The hydration and mechanical properties of PulMA were demonstrated to be tunable through modulation of the degree of methacrylation and gel concentration. Cells encapsulated in PulMA exhibited excellent viability. Interestingly, while cells did not elongate in PulMA hydrogels, cells proliferated and organized into clusters, the size of which could be controlled by the hydrogel composition. By mixing with gelatin methacrylate (GelMA), the biological properties of PulMA could be enhanced as demonstrated by cells readily attaching to, proliferating, and elongating within the PulMA/GelMA composite hydrogels. These data suggest that PulMA hydrogels could be useful for creating complex, cell-responsive microtissues, especially for applications that require controlled cell clustering and proliferation.     

Interface‐Directed Self‐Assembly of Cell‐Laden Microgels

Cell‐laden hydrogels show great promise for creating engineered tissues. However, a major shortcoming with these systems has been the inability to fabricate structures with controlled micrometer‐scale features on a biologically relevant length scale. In this Full Paper, a rapid method is demonstrated for creating centimeter‐scale, cell‐laden hydrogels through the assembly of shape‐controlled microgels or a liquid–air interface. Cell‐laden microgels of specific shapes are randomly placed on the surface of a high‐density, hydrophobic solution, induced to aggregate and then crosslinked into macroscale tissue‐like structures. The resulting assemblies are cell‐laden hydrogel sheets consisting of tightly packed, ordered microgel units. In addition, a hierarchical approach creates complex multigel building blocks, which are then assembled into tissues with precise spatial control over the cell distribution. The results demonstrate that forces at an air–liquid interface can be used to self‐assemble spatially controllable, cocultured tissue‐like structures.     

A Model of Mechanotransduction in Polyvinyl Alcohol-Based Composite Hydrogels for Regulating Musculoskeletal Differentiation

In regenerative medicine, extracellular matrix (ECM)-inspired materials are currently being explored to imitate mechanotransduction pathways and control cell fate. In musculoskeletal tissue regeneration, enhancing mechano-biological signals require biomaterials that are both biocompatible and viscoelastic and can retain water content. Herein, based on these requirements, various polyvinyl alcohol (PVA)-based composite hydrogels, reinforced by polyhydroxy butyrate (PHB) nanofibers, are proposed to differentiate equine adipose-derived stem cells for musculoskeletal regeneration. To study the role of fiber embedding in improving scaffold properties, different nanofiber assemblies, including chopped short ones with random orientation (PVA_S), single-layer (PVA_L1), and double-layer membranes (PVA_L2) are positioned into the PVA matrix. PHB reinforcements negatively affect swelling and positively enhanced phase transition temperatures and crystallinity of PVA hydrogel. According to mechanical analysis results, compositing with PHB nanofibrous layers strengthen the PVA matrix due to some restrictions on PVA chain mobility. Gene expression investigations also reveal that higher matrix stiffness after layering with two PHB membranes (PVA_L2) promotes osteogenesis, while the random addition of short-chapped fibers (PVA_S) facilitate tenogenic differentiation. As a consequence of the findings, fiber placement is crucial to the mechanical properties of composite hydrogels that ultimately control musculoskeletal differentiation signals through mechanosensing pathways.